Biochemical analysis unit and biochemical analyzing method using the same

ABSTRACT

A biochemical analysis unit includes a substrate made of a material capable of attenuating radiation energy and/or light energy and formed with a plurality of holes, and a plurality of absorptive regions formed by forming an absorptive region in every hole. According to the thus constituted biochemical analysis unit, even in the case where the absorptive regions are formed at a high density, when a stimulable phosphor layer formed on a stimulable phosphor sheet is exposed to a radioactive labeling substance contained in the plurality of absorptive regions, electron beams (β rays) released from the radioactive labeling substance contained in the individual absorptive regions are reliably prevented from being scattered in the substrate and advancing to regions of the stimulable phosphor layer that should be exposed to electron beams released from absorptive regions formed in neighboring holes. Therefore, it is possible to efficiently prevent noise caused by the scattering of electron beams released from the radioactive labeling substance from being generated in biochemical analysis data produced by irradiating the stimulable phosphor layer exposed to the radioactive labeling substance with a stimulating ray and photoelectrically detecting stimulated emission released from the stimulable phosphor layer and to produce biochemical analysis data having a high quantitative accuracy.

BACKGROUND OF THE INVENTION

[0001] The present invention relates to a biochemical analysis unit anda biochemical analyzing method using the same and, particularly, to abiochemical analysis unit and a biochemical analyzing method which canprevent noise caused by the scattering of electron beams released from aradioactive labeling substance from being generated in biochemicalanalysis data even in the case of forming spots of specific bindingsubstances on the surface of a carrier at a high density, which canspecifically bind with a substance derived from a living organism andwhose sequence, base length, composition and the like are known,specifically binding the spot-like specific binding substances with asubstance derived from a living organism labeled with a radioactivesubstance to selectively label the spot-like specific binding substanceswith the radioactive substance, thereby obtaining a biochemical analysisunit, superposing the thus obtained biochemical analysis unit and astimulable phosphor layer, exposing the stimulable phosphor layer to theradioactive labeling substance, irradiating the stimulable phosphorlayer with a stimulating ray to excite the stimulable phosphor,photoelectrically detecting the stimulated emission released from thestimulable phosphor layer to produce biochemical analysis data, andanalyzing the substance derived from a living organism; and can preventnoise caused by the scattering of chemiluminescent emission and/orfluorescence released from a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand/or a fluorescent substance from being generated in biochemicalanalysis data even in the case of forming spots of specific bindingsubstances on the surface of a carrier at high density, which canspecifically bind with a substance derived from a living organism andwhose sequence, base length, composition and the like are known,specifically binding the spot-like specific binding substance with asubstance derived from a living organism labeled with, in addition to aradioactive labeling substance or instead of a radioactive labelingsubstance, a labeling substance which generates chemiluminescentemission when it contacts a chemiluminescent substrate and/or afluorescent substance to selectively label the spot-like specificbinding substances therewith, thereby obtaining a biochemical analysisunit, photoelectrically detecting chemiluminescent emission and/orfluorescence released from the biochemical analysis unit to producebiochemical analysis data, and analyzing the substance derived from aliving organism.

DESCRIPTION OF THE PRIOR ART

[0002] An autoradiographic analyzing system using as a detectingmaterial for detecting radiation a stimulable phosphor which can absorb,store and record the energy of radiation when it is irradiated withradiation and which, when it is then stimulated by an electromagneticwave having a specified wavelength, can release stimulated emissionwhose light amount corresponds to the amount of radiation with which itwas irradiated is known, which comprises the steps of introducing aradioactively labeled substance into an organism, using the organism ora part of the tissue of the organism as a specimen, superposing thespecimen and a stimulable phosphor sheet formed with a stimulablephosphor layer for a certain period of time, storing and recordingradiation energy in a stimulable phosphor contained in the stimulablephosphor layer, scanning the stimulable phosphor layer with anelectromagnetic wave to excite the stimulable phosphor,photoelectrically detecting the stimulated emission released from thestimulable phosphor to produce digital image signals, effecting imageprocessing on the obtained digital image signals, and reproducing animage on displaying means such as a CRT or the like or a photographicfilm (see, for example, Japanese Patent Publication No. 1-60784,Japanese Patent Publication No. 1-60782, Japanese Patent Publication No.4-3952 and the like).

[0003] Unlike the system using a photographic film, according to theautoradiographic analyzing system using the stimulable phosphor as adetecting material, development, which is chemical processing, becomesunnecessary. Further, it is possible reproduce a desired image byeffecting image processing on the obtained image data and effectquantitative analysis using a computer. Use of a stimulable phosphor inthese processes is therefore advantageous.

[0004] On the other hand, a fluorescence analyzing system using afluorescent substance as a labeling substance instead of a radioactivelabeling substance in the autoradiographic analyzing system is known.According to this system, it is possible to study a genetic sequence,study the expression level of a gene, and to effect separation oridentification of protein or estimation of the molecular weight orproperties of protein or the like. For example, this system can performa process including the steps of distributing a plurality of DNAfragments on a gel support by means of electrophoresis after afluorescent dye was added to a solution containing a plurality of DNAfragments to be distributed, or distributing a plurality of DNAfragments on a gel support containing a fluorescent dye, or dipping agel support on which a plurality of DNA fragments have been distributedby means of electrophoresis in a solution containing a fluorescent dye,thereby labeling the electrophoresed DNA fragments, exciting thefluorescent dye by a stimulating ray to cause it to release fluorescentlight, detecting the released fluorescent light to produce an image anddetecting the distribution of the DNA fragments on the gel support. Thissystem can also perform a process including the steps of distributing aplurality of DNA fragments on a gel support by means of electrophoresis,denaturing the DNA fragments, transferring at least a part of thedenatured DNA fragments onto a transfer support such as a nitrocellulosesupport by the Southern-blotting method, hybridizing a probe prepared bylabeling target DNA and DNA or RNA complementary thereto with thedenatured DNA fragments, thereby selectively labeling only the DNAfragments complementary to the probe DNA or probe RNA, exciting thefluorescent dye by a stimulating ray to cause it to release fluorescentlight, detecting the released fluorescent light to produce an image anddetecting the distribution of the target DNA on the transfer support.This system can further perform a process including the steps ofpreparing a DNA probe complementary to DNA containing a target genelabeled by a labeling substance, hybridizing it with DNA on a transfersupport, combining an enzyme with the complementary DNA labeled by alabeling substance, causing the enzyme to contact a fluorescentsubstance, transforming the fluorescent substance to a fluorescentsubstance having fluorescent light releasing property, exciting the thusproduced fluorescent substance by a stimulating ray to releasefluorescent light, detecting the fluorescent light to produce an imageand detecting the distribution of the target DNA on the transfersupport. This fluorescence detecting system is advantageous in that agenetic sequence or the like can be easily detected without using aradioactive substance.

[0005] Similarly, there is known a chemiluminescence detecting systemcomprising the steps of fixing a substance derived from a livingorganism such as a protein or a nucleic acid sequence on a support,selectively labeling the substance derived from a living organism with alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate, contacting the substance derivedfrom a living organism and selectively labeled with the labelingsubstance and the chemiluminescent substrate, photoelectricallydetecting the chemiluminescent emission in the wavelength of visiblelight generated by the contact of the chemiluminescent substrate and thelabeling substance to produce digital image signals, effecting imageprocessing thereon, and reproducing a chemiluminescent image on adisplay means such as a CRT or a recording material such as aphotographic film, thereby obtaining information relating to the highmolecular substance such as genetic information

[0006] Further, a micro-array analyzing system has been recentlydeveloped, which comprises the steps of using a spotting device to dropat different positions on the surface of a carrier such as a slide glassplate, a membrane filter or the like specific binding substances, whichcan specifically bind with a substance derived from a living organismsuch as a hormone, tumor marker, enzyme, antibody, antigen, abzyme,other protein, a nuclear acid, cDNA, DNA, RNA or the like and whosesequence, base length, composition and the like are known, therebyforming a number of independent spots, specifically binding the specificbinding substances using a hybridization method or the like with asubstance derived from a living organism such as a hormone, tumormarker, enzyme, antibody, antigen, abzyme, other protein, a nuclearacid, cDNA, DNA or mRNA, which is gathered from a living organism byextraction, isolation or the like or is further subjected to chemicalprocessing, chemical modification or the like and which is labeled witha labeling substance such as a fluorescent substance, dye or the like,thereby forming a micro-array, irradiating the micro-array with astimulating ray, photoelectrically detecting light such as afluorescence emitted from a labeling substance such as a fluorescentsubstance, dye or the like, and analyzing the substance derived from aliving organism. This micro-array analyzing system is advantageous inthat a substance derived from a living organism can be analyzed in ashort time period by forming a number of spots of specific bindingsubstances at different positions of the surface of a carrier such as aslide glass plate at high density and hybridizing them with a substancederived from a living organism and labeled with a labeling substance.

[0007] In addition, a macro-array analyzing system using a radioactivelabeling substance as a labeling substance has been further developed,which comprises the steps of using a spotting device to drop atdifferent positions on the surface of a carrier such as a membranefilter or the like specific binding substances, which can specificallybind with a substance derived from a living organism such as a hormone,tumor marker, enzyme, antibody, antigen, abzyme, other protein, anuclear acid, cDNA, DNA, RNA or the like and whose sequence, baselength, composition and the like are known, thereby forming a number ofindependent spots, specifically binding the specific binding substanceusing a hybridization method or the like with a substance derived from aliving organism such as a hormone, tumor marker, enzyme, antibody,antigen, abzyme, other protein, a nuclear acid, cDNA, DNA or mRNA, whichis gathered from a living organism by extraction, isolation or the likeor is further subjected to chemical processing, chemical modification orthe like and which is labeled with a radioactive labeling substance,thereby forming a macro-array, superposing the macro-array and astimulable phosphor sheet formed with a stimulable phosphor layer,exposing the stimulable phosphor layer to a radioactive labelingsubstance, irradiating the stimulable phosphor layer with a stimulatingray to excite the stimulable phosphor, photoelectrically detecting thestimulated emission released from the stimulable phosphor to producebiochemical analysis data, and analyzing the substance derived from aliving organism.

[0008] However, in the macro-array analyzing system using a radioactivelabeling substance as a labeling substance, when the stimulable phosphorlayer is exposed to a radioactive labeling substance, since radiationenergy of the radioactive labeling substance contained in spots formedon the surface of a carrier such as a membrane filter is very large,electron beams released from the radioactive labeling substancecontained in the individual spots are scattered in the carrier such as amembrane filter, thereby impinging on regions of the stimulable phosphorlayer that should be exposed to the radioactive labeling substancecontained in neighboring spots, or electron beams released from theradioactive labeling substance contained in the individual spots arescattered and mixed with the electron beams released from theradioactive labeling substance contained in neighboring spots and thenimpinge on regions of the stimulable phosphor layer to generate noise inbiochemical analysis data produced by photoelectrically detectingstimulated emission and to lower the accuracy of biochemical analysiswhen a substance derived from a living organism is analyzed byquantifying the radiation amount of each spot. The accuracy ofbiochemical analysis is markedly degraded when spots are formed closelyto each other at high density.

[0009] In order to solve these problems by preventing noise caused bythe scattering of electron beams released from radioactive labelingsubstance contained in neighboring spots, it is inevitably required toincrease the distance between neighboring spots and this makes thedensity of the spots lower and the test efficiency lower.

[0010] Further, in the field of biochemical analysis, it is oftenrequired to analyze a substance derived from a living organism byspecifically binding, using a hybridization method or the like, specificbinding substances spot-like formed at different positions on thesurface of a carrier such as a membrane filter or the like, which canspecifically bind with a substance derived from a living organism suchas a hormone, tumor marker, enzyme, antibody, antigen, abzyme, otherprotein, a nuclear acid, cDNA, DNA, RNA or the like and whose sequence,base length, composition and the like are known, with a substancederived from a living organism labeled with, in addition to aradioactive labeling substance, a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand/or a fluorescent substance, and after exposing a stimulable phosphorlayer to the radioactive labeling substance or prior to exposing astimulable phosphor layer to the radioactive labeling substance, causingit to contact a chemiluminescent substrate, thereby photoelectricallydetecting the chemiluminescent emission in the wavelength of visiblelight, and/or irradiating it with a stimulating ray, therebyphotoelectrically detecting fluorescence released from a fluorescentsubstance. In these cases, chemiluminescent emission or fluorescencereleased from spots is scattered in the carrier such as a membranefilter or chemiluminescent emission or fluorescence released from anyparticular spot is scattered and mixed with chemiluminescent emission orfluorescence released from neighboring spots, thereby generating noisein biochemical analysis data produced by photoelectrically detectingchemiluminescent emission and/or fluorescence.

SUMMARY OF THE INVENTION

[0011] It is therefore an object of the present invention to provide abiochemical analysis unit which can prevent noise caused by thescattering of electron beams released from a radioactive labelingsubstance from being generated in biochemical analysis data even in thecase of forming spots of specific binding substances on the surface of acarrier at high density, which can specifically bind with a substancederived from a living organism and whose sequence, base length,composition and the like are known, specifically binding the spot-likespecific binding substance with a substance derived from a livingorganism and labeled with a radioactive substance to selectively labelthe spot-like specific binding substances with a radioactive substance,thereby obtaining a biochemical analysis unit, superposing the thusobtained biochemical analysis unit and a stimulable phosphor layer,exposing the stimulable phosphor layer to the radioactive labelingsubstance, irradiating the stimulable phosphor layer with a stimulatingray to excite the stimulable phosphor, photoelectrically detecting thestimulated emission released from the stimulable phosphor layer toproduce biochemical analysis data, and analyzing the substance derivedfrom a living organism.

[0012] It is another object of the present invention to provide abiochemical analysis unit which can prevent noise caused by thescattering of chemiluminescent emission and/or fluorescence releasedfrom a labeling substance which generates chemiluminescent emission whenit contacts a chemiluminescent substrate and/or a fluorescent substancefrom being generated in biochemical analysis data even in the case offorming spots of specific binding substances on the surface of a carrierat high density, which can specifically bind with a substance derivedfrom a living organism and whose sequence, base length, composition andthe like are known, specifically binding the spot-like specific bindingsubstance with a substance derived from a living organism and labeledwith, in addition to a radioactive labeling substance or instead of aradioactive labeling substance, a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand/or a fluorescent substance to selectively label the spot-likespecific binding substances therewith, thereby obtaining a biochemicalanalysis unit, photoelectrically detecting chemiluminescent emissionand/or fluorescence released from the biochemical analysis unit toproduce biochemical analysis data, and analyzing the substance derivedfrom a living organism.

[0013] It is a further object of the present invention to provide abiochemical analyzing method which can effect quantitative biochemicalanalysis with high accuracy by producing biochemical analysis data basedon a biochemical analysis unit obtained by forming spots of specificbinding substances on the surface of a carrier at high density, whichcan specifically bind with a substance derived from a living organismand whose sequence, base length, composition and the like are known,specifically binding the spot-like specific binding substances with asubstance derived from a living organism and labeled with a radioactivelabeling substance, a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand/or a fluorescent substance, thereby selectively labeling thespot-like specific binding substances therewith.

[0014] The above other objects of the present invention can beaccomplished by a biochemical analysis unit comprising a substrate madeof a material capable of attenuating radiation energy and/or lightenergy and formed with a plurality of holes, and a plurality ofabsorptive regions formed by forming an absorptive region in every hole.

[0015] In one mode of use of the biochemical analysis unit according tothis aspect of the present invention, specific binding substances, whichcan specifically bind with a substance derived from a living organismand whose sequence, base length, composition and the like are known, arespotted in the absorption regions in a number of the holes formed in thebiochemical analysis unit at a high density and a substance derived froma living organism and labeled with a radioactive substance isspecifically bound with the specific binding substances, therebyselectively labeling the plurality of absorptive regions therewith. Thebiochemical analysis unit is then disposed so as to face a stimulablephosphor layer, thereby exposing the stimulable phosphor layer to theradioactive labeling substance contained in the plurality of absorptiveregions. Since the substrate of the biochemical analysis unit is made ofa material capable of attenuating radiation energy, electron beams (βrays) released from the radioactive labeling substance contained in theindividual absorptive regions are reliably prevented from beingscattered in the substrate and advancing to regions of the stimulablephosphor layer that should be exposed to electron beams released fromabsorptive regions formed in neighboring holes. Therefore, it ispossible to efficiently prevent noise caused by the scattering ofelectron beams released from the radioactive labeling substance frombeing generated in biochemical analysis data produced by irradiating thestimulable phosphor layer exposed to the radioactive labeling substancewith a stimulating ray and photoelectrically detecting stimulatedemission released from the stimulable phosphor layer and to producebiochemical analysis data having a high quantitative accuracy.

[0016] In another mode of use of the biochemical analysis unit accordingto this aspect of the present invention, specific binding substances,which can specifically bind with a substance derived from a livingorganism and whose sequence, base length, composition and the like areknown, are spotted in absorption regions in a number of holes formed ina biochemical analysis unit at a high density and a substance derivedfrom a living organism and labeled with a labeling substance whichgenerates chemiluminescent emission when it contacts a chemiluminescentsubstrate and/or a fluorescent substance, instead of with a radioactivelabeling substance, thereby selectively labeling the plurality ofabsorptive regions therewith. Biochemical data are then produced byphotoelectrically detecting chemiluminescent emission generated by thecontact of a chemiluminescent substrate and the labeling substanceand/or fluorescence released from the fluorescent substance in responseto irradiation by a stimulating ray. Since the substrate of thebiochemical analysis unit is made of a material capable of attenuatingradiation energy, it is possible to reliably prevent chemiluminescentemission and/or fluorescence from being scattered in the substrate and,therefore, it is possible to efficiently prevent noise caused by thescattering of chemiluminescent emission and/or fluorescence from beinggenerated in biochemical analysis data produced by photoelectricallydetecting chemiluminescent emission and/or fluorescence.

[0017] In another mode of use of the biochemical analysis unit accordingto this aspect the present invention, specific binding substances, whichcan specifically bind with a substance derived from a living organismand whose sequence, base length, composition and the like are known, arespotted in absorption regions in a number of holes formed in abiochemical analysis unit at a high density and a substance derived froma living organism and labeled with, in addition to a radioactivelabeling substance, a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand/or a fluorescent substance, thereby selectively labeling theplurality of absorptive regions therewith. The biochemical analysis unitis then disposed so as to face a stimulable phosphor layer, therebyexposing the stimulable phosphor layer to a radioactive labelingsubstance contained in the plurality of absorptive regions. Since thesubstrate of the biochemical analysis unit is made of a material capableof attenuating radiation energy, electron beams (β rays) released fromthe radioactive labeling substance contained in the individualabsorptive regions are reliably prevented from being scattered in thesubstrate and advancing to regions of the stimulable phosphor layer thatshould be exposed to electron beams released from absorptive regionsformed in neighboring holes. Therefore, it is possible to efficientlyprevent noise caused by the scattering of electron beams released fromthe radioactive labeling substance from being generated in biochemicalanalysis data produced by irradiating the stimulable phosphor layerexposed to the radioactive labeling substance with a stimulating ray andphotoelectrically detecting stimulated emission released from thestimulable phosphor layer and to produce biochemical analysis datahaving a high quantitative accuracy. On the other hand, when biochemicaldata are produced by photoelectrically detecting chemiluminescentemission generated by the contact of a chemiluminescent substrate andthe labeling substance and/or fluorescence released from the fluorescentsubstance in response to irradiation by a stimulating ray, the fact thatthe substrate is made of a material capable of attenuating radiationenergy and light energy makes it possible to reliably preventchemiluminescent emission and/or fluorescence from being scattered inthe substrate and, therefore, it is possible to efficiently preventnoise caused by the scattering of chemiluminescent emission and/orfluorescence from being generated in biochemical analysis data producedby photoelectrically detecting chemiluminescent emission and/orfluorescence.

[0018] The above and other objects of the present invention can also beaccomplished by a biochemical analysis unit comprising a substrate madeof a material capable of attenuating radiation energy and/or lightenergy and formed with a plurality of holes, and a plurality ofabsorptive regions formed by forming an absorptive region in every hole,the plurality of absorptive regions being selectively labeled with atleast one kind of labeling substance selected from a group consisting ofa radioactive labeling substance, a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand a fluorescent substance by spotting specific binding substanceswhose sequence, base length, composition and the like are known thereinand specifically binding a substance derived from a living organism andlabeled with at least one kind of said labeling substance with thespecific binding substances.

[0019] In the present invention, the case where a substance derived froma living organism is labeled with a fluorescent substance as termedherein includes the case where a substance derived from a livingorganism is labeled with a fluorescent dye and the case where asubstance derived from a living organism is labeled with a fluorescentsubstance obtained by combining an enzyme with a labeled specimen,contacting the enzyme and a fluorescent substrate, thereby changing thefluorescent substrate to a fluorescent substance capable of emittingfluorescent light.

[0020] According to this aspect of the present invention, the pluralityof absorptive regions are selectively labeled with at least one kind oflabeling substance selected from a group consisting of a radioactivelabeling substance, a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand a fluorescent substance by spotting therein specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known and specifically binding a substance derived from aliving organism and labeled with at least one kind of said labelingsubstance with the specific binding substances. In this case, since thesubstrate is made of a material capable of attenuating radiation energy,when the biochemical analysis unit is disposed so as to face astimulable phosphor layer, thereby exposing the stimulable phosphorlayer to a radioactive labeling substance contained in the plurality ofabsorptive regions, electron beams (β rays) released from theradioactive labeling substance contained in the individual absorptiveregions are reliably prevented from being scattered in the substrate andadvancing to regions of the stimulable phosphor layer that should beexposed to electron beams released from absorptive regions formed inneighboring holes. Therefore, it is possible to efficiently preventnoise caused by the scattering of electron beams released from theradioactive labeling substance from being generated in biochemicalanalysis data produced by irradiating the stimulable phosphor layerexposed to the radioactive labeling substance with a stimulating ray andphotoelectrically detecting stimulated emission released from thestimulable phosphor layer and to produce biochemical analysis datahaving a high quantitative accuracy.

[0021] Further, according to this aspect of the present invention, sincethe substrate is made of a material capable of attenuating light energy,when biochemical data are produced by photoelectrically detectingchemiluminescent emission generated by the contact of a chemiluminescentsubstrate and the labeling substance and/or fluorescence released fromthe fluorescent substance in response to the irradiation of astimulating ray, it is possible to reliably prevent chemiluminescentemission and/or fluorescence from being scattered in the substrate and,therefore, it is possible to efficiently prevent noise caused by thescattering of chemiluminescent emission and/or fluorescence from beinggenerated in biochemical analysis data produced by photoelectricallydetecting chemiluminescent emission and/or fluorescence.

[0022] Furthermore, according to this aspect of the present invention,the substrate is made of a material capable of attenuating radiationenergy and light energy and the plurality of absorptive regions areselectively labeled with at least one kind of labeling substancesselected from a group consisting of a radioactive labeling substance, alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate and a fluorescent substance byspotting therein specific binding substances which can specifically bindwith a substance derived from a living organism and whose sequence, baselength, composition and the like are known, and specifically binding asubstance derived from a living organism and labeled with at least onekind of said labeling substances with the specific binding substances.Therefore, when the biochemical analysis unit is disposed so as to facea stimulable phosphor layer, thereby exposing the stimulable phosphorlayer to the radioactive labeling substance contained in the pluralityof absorptive regions, electron beams (β rays ) released from theradioactive labeling substance contained in the individual absorptiveregions are reliably prevented from being scattered in the substrate andadvancing to regions of the stimulable phosphor layer that should beexposed to electron beams released from absorptive regions formed inneighboring holes. Therefore, it is possible to efficiently preventnoise caused by the scattering of electron beams released from theradioactive labeling substance from being generated in biochemicalanalysis data produced by irradiating the stimulable phosphor layerexposed to the radioactive labeling substance with a stimulating ray andphotoelectrically detecting stimulated emission released from thestimulable phosphor layer and to produce biochemical analysis datahaving a high quantitative accuracy. On the other hand, when biochemicaldata are produced by photoelectrically detecting chemiluminescentemission generated by the contact of a chemiluminescent substrate andthe labeling substance and/or fluorescence released from the fluorescentsubstance in response to the irradiation of a stimulating ray, the factthat the substrate is made of a material capable of attenuatingradiation energy and light energy makes it possible to reliably preventchemiluminescent emission and/or fluorescence from being scattered inthe substrate and, therefore, it is possible to efficiently preventnoise caused by the scattering of chemiluminescent emission and/orfluorescence from being generated in biochemical analysis data producedby photoelectrically detecting chemiluminescent emission and/orfluorescence. Furthermore, when biochemical data are produced byphotoelectrically detecting chemiluminescent emission generated by thecontact of a chemiluminescent substrate and the labeling substanceand/or fluorescence released from the fluorescent substance in responseto the irradiation of a stimulating ray, the fact that the substrate ismade of a material capable of attenuating radiation energy and lightenergy makes it possible to reliably prevent chemiluminescent emissionand/or fluorescence from being scattered in the substrate and,therefore, it is possible to efficiently prevent noise caused by thescattering of chemiluminescent emission and/or fluorescence from beinggenerated in biochemical analysis data produced by photoelectricallydetecting chemiluminescent emission and/or fluorescence.

[0023] In a preferred aspect of the present invention, the plurality ofabsorptive regions are formed by charging an absorptive material in theplurality of holes formed in the substrate.

[0024] In a preferred aspect of the present invention, each of theplurality of holes is formed as a through-hole.

[0025] In another preferred aspect of the present invention, each of theplurality of holes is formed as a recess.

[0026] In a preferred aspect of the present invention, the substrate isformed with a gripping portion by which the substrate can be gripped.

[0027] According to this preferred aspect of the present invention,since the substrate is formed with a gripping portion by which thesubstrate can be gripped, the biochemical analysis unit can be veryeasily handled when specific binding substances are spotted, duringhybridization or during exposure operation.

[0028] The above and other objects of the present invention can also beaccomplished by a biochemical analysis unit comprising an absorptivesubstrate formed of an absorptive material and a perforated plate formedwith a plurality of through-holes and made of a material capable ofattenuating radiation energy and light energy, the perforated platebeing closely contacted with at least one surface of the absorptivesubstrate to form a plurality of absorptive regions of the absorptivesubstrate in the plurality of through-holes formed in the perforatedplate.

[0029] In one mode of use of the biochemical analysis unit according tothis aspect of the present invention, specific binding substances, whichcan specifically bind with a substance derived from a living organismand whose sequence, base length, composition and the like are known, arespotted in the plurality of absorption regions formed in the absorptivesubstrate in the plurality of through-holes of the perforated plate at ahigh density and a substance derived from a living organism and labeledwith a radioactive substance is specifically bound with the specificbinding substances, thereby selectively labeling the specific bindingsubstances therewith. The absorptive substrate is the disposed so as toface a stimulable phosphor layer via the perforated plate, therebyexposing the stimulable phosphor layer to the radioactive labelingsubstance contained in the plurality of absorptive regions. Since theperforated plate is made of a material capable of attenuating radiationenergy, electron beams (β rays) released from the radioactive labelingsubstance contained in the individual absorptive regions and electronbeams released from neighboring absorptive regions can be reliablyseparated by the perforated plate, thereby reliably preventing electronbeams released from the radioactive labeling substance contained in theindividual absorptive regions from advancing to regions of thestimulable phosphor layer that should be exposed to electron beamsreleased from neighboring absorptive regions. Therefore, it is possibleto efficiently prevent noise caused by the scattering of electron beamsreleased from the radioactive labeling substance from being generated inbiochemical analysis data produced by irradiating the stimulablephosphor layer exposed to the radioactive labeling substance with astimulating ray and photoelectrically detecting stimulated emissionreleased from the stimulable phosphor layer and to produce biochemicalanalysis data having a high quantitative accuracy.

[0030] In another mode of use of the biochemical analysis unit accordingto this aspect of the present invention, specific binding substances,which can specifically bind with a substance derived from a livingorganism and whose sequence, base length, composition and the like areknown, are spotted in the plurality of absorption regions formed in theabsorptive substrate in the plurality of through-holes of the perforatedplate at a high density and a substance derived from a living organismand labeled with a labeling substance capable of generatingchemiluminescent emission when it contacts a chemiluminescent substrateand/or a fluorescent substance, thereby selectively labeling thespecific binding substances therewith. Biochemical analysis data arethen produced by photoelectrically detecting chemiluminescent emissiongenerated by bringing a chemiluminescent substrate into contact with theabsorptive substrate via the perforated plate and/or fluorescencereleased from the fluorescent substance in response to irradiation by astimulating ray via the perforated plate. Since the perforated plate ismade of a material capable of attenuating light energy, chemiluminescentemission and/or fluorescence released from the individual absorptiveregions and chemiluminescent emission and/or fluorescence released fromneighboring absorptive regions can be reliably separated by theperforated plate, thereby reliably preventing chemiluminescent emissionand/or fluorescence released from the individual absorptive regions frombeing scattered. Therefore, it is possible to efficiently prevent noisecaused by the scattering of chemiluminescent emission and/orfluorescence from being generated in biochemical analysis data producedby photoelectrically detecting chemiluminescence emission and/orfluorescence.

[0031] In another mode of use of the biochemical analysis unit accordingto this aspect of the invention, specific binding substances, which canspecifically bind with a substance derived from a living organism andwhose sequence, base length, composition and the like are known, arespotted in the absorption regions formed in the plurality of absorptivesubstrate in the plurality of through-holes of the perforated plate at ahigh density and a substance derived from a living organism and labeledwith at least one kind of labeling substance selected from a groupconsisting of a radioactive labeling substance, a labeling substancecapable of generating chemiluminescent emission when it contacts achemiluminescent substrate and/or a fluorescent substance, therebyselectively labeling the specific binding substances therewith. Theabsorptive substrate is then disposed so as to face a stimulablephosphor layer via the perforated plate, thereby exposing the stimulablephosphor layer to a radioactive labeling substance contained in theplurality of absorptive regions. Since the perforated plate is made of amaterial capable of attenuating radiation energy and light energy,electron beams (β rays ) released from the radioactive labelingsubstance contained in the individual absorptive regions and electronbeams released from neighboring absorptive regions can be reliablyseparated by the perforated plate, thereby reliably preventing electronbeams released from the radioactive labeling substance contained in theindividual absorptive regions from advancing to regions of thestimulable phosphor layer that should be exposed to electron beamsreleased from neighboring absorptive regions. Therefore, it is possibleto efficiently prevent noise caused by the scattering of electron beamsreleased from the radioactive labeling substance from being generated inbiochemical analysis data produced by irradiating the stimulablephosphor layer exposed to the radioactive labeling substance with astimulating ray and photoelectrically detecting stimulated emissionreleased from the stimulable phosphor layer and to produce biochemicalanalysis data having a high quantitative accuracy. On the other hand,when biochemical analysis data are produced by photoelectricallydetecting chemiluminescent emission generated by bringing achemiluminescent substrate into contact with the absorptive substratevia the perforated plate and/or fluorescence released from thefluorescent substance in response to irradiation by a stimulating rayvia the perforated plate, since the perforated plate is made of amaterial capable of attenuating radiation energy and light energy,chemiluminescent emission and/or fluorescence released from theindividual absorptive regions and chemiluminescent emission and/orfluorescence released from neighboring absorptive regions can bereliably separated by the perforated plate, thereby reliably preventingchemiluminescent emission and/or fluorescence released from theindividual absorptive regions from being scattered. Therefore, it ispossible to efficiently prevent noise caused by the scattering ofchemiluminescent emission and/or fluorescence from being generated inbiochemical analysis data produced by photoelectrically detectingchemiluminescence emission and/or fluorescence.

[0032] In a preferred aspect of the present invention, perforated platesare in close contact with the both surfaces of the absorptive substrate.

[0033] According to this preferred aspect of the present invention,since perforated plates are in close contact with the both surfaces ofthe absorptive substrate, the strength of the biochemical analysis unitcan be improved.

[0034] In a preferred aspect of the present invention, the perforatedplate is formed with a gripping portion by which the perforated platecan be gripped.

[0035] According to this preferred aspect of the present invention,since the perforated plate is formed with a gripping portion by whichthe perforated plate can be gripped, the biochemical analysis unit canbe very easily handled when specific binding substances are spotted,during hybridization or during exposure operation.

[0036] In a preferred aspect of the present invention, the specificbinding substances are spotted through the plurality of through-holes inthe plurality of absorptive regions formed on the absorptive substrate.

[0037] In a preferred aspect of the present invention, the plurality ofabsorptive regions are selectively labeled with at least one kind oflabeling substances selected from a group consisting of a radioactivelabeling substance, a labeling substance capable of generatingchemiluminescent emission when it contacts a chemiluminescent substrateand/or a fluorescent substance by spotting specific binding substanceswhose sequence, base length, composition and the like are known thereinand hybridizing a substance derived from a living organism and labeledwith at least one kind of labeling substance with the specific bindingsubstances.

[0038] The above and other objects of the present invention can also beaccomplished by a biochemical analyzing method comprising the steps ofpreparing a biochemical analysis unit by spotting specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, in a plurality of absorptive regions, each of which isformed in a plurality of holes formed in a substrate made of a materialcapable of attenuating radiation energy and specifically binding asubstance derived from a living organism and labeled with a radioactivelabeling substance with the specific binding substances, superposing thebiochemical analysis unit on a stimulable phosphor sheet in which astimulable phosphor layer is formed so that the stimulable phosphorlayer faces the plurality of absorptive regions, thereby exposing thestimulable phosphor layer to the radioactive labeling substancecontained in the plurality of absorptive regions, irradiating thestimulable phosphor layer exposed to the radioactive labeling substancewith a stimulating ray, thereby exciting stimulable phosphor containedin the stimulable phosphor layer, photoelectrically detecting stimulatedemission released from the stimulable phosphor contained in thestimulable phosphor layer, thereby producing biochemical analysis data,and effecting biochemical analysis based on the biochemical analysisdata.

[0039] According to this aspect of the present invention, thebiochemical analysis unit is prepared by spotting specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, in a plurality of absorptive regions formed in aplurality of holes formed in a substrate made of a material capable ofattenuating radiation energy and specifically binding a substancederived from a living organism and labeled with a radioactive labelingsubstance with the specific binding substances, thereby selectivelylabeling the plurality of absorptive regions. The biochemical analysisunit is then superposed on a stimulable phosphor sheet in which astimulable phosphor layer is formed so that the stimulable phosphorlayer faces the plurality of absorptive regions, thereby exposing thestimulable phosphor layer to the radioactive labeling substancecontained in the plurality of absorptive regions. Since the substrate ofthe biochemical analysis unit is made of a material capable ofattenuating radiation energy, electron beams (β rays) released from theradioactive labeling substance contained in the individual absorptiveregions are reliably prevented from being scattered in the substrate andscattered electron beams are prevented from advancing to regions of thestimulable phosphor layer that should be exposed to electron beamsreleased from the radioactive labeling substance contained absorptiveregions formed in neighboring holes. Therefore, it is possible toefficiently prevent noise caused by the scattering of electron beamsreleased from the radioactive labeling substance from being generated inbiochemical analysis data produced by irradiating the stimulablephosphor layer exposed to the radioactive labeling substance with astimulating ray and photoelectrically detecting stimulated emissionreleased from the stimulable phosphor layer and to effect biochemicalanalysis with high quantitative accuracy.

[0040] In a preferred aspect of the present invention, the plurality ofabsorptive regions are formed by charging an absorptive material in theplurality of holes formed in the substrate of the biochemical analysisunit.

[0041] In a preferred aspect of the present invention, the plurality ofholes formed in the substrate of the biochemical analysis unit areconstituted as through-holes.

[0042] In another preferred aspect of the present invention, theplurality of holes formed in the substrate of the biochemical analysisunit are constituted as recesses.

[0043] In a preferred aspect of the present invention, a plurality ofdot-like stimulable phosphor layer regions are formed spaced-apart fromeach other in the stimulable phosphor sheet in the same pattern as thatof the plurality of holes formed in the substrate of the biochemicalanalysis unit and the biochemical analysis unit and the stimulablephosphor sheet are superposed on each other so that each of theplurality of dot-like stimulable phosphor layer regions faces one of theplurality of absorptive regions in the plurality of holes formed in thesubstrate of the biochemical analysis unit, thereby exposing theplurality of dot-like stimulable phosphor layer regions of thestimulable phosphor sheet to the radioactive labeling substancecontained in the plurality of absorptive regions.

[0044] According to this preferred aspect of the present invention, theplurality of dot-like stimulable phosphor layer regions are formedspaced-apart in the stimulable phosphor sheet in the same pattern asthat of the plurality of holes formed in the substrate of thebiochemical analysis unit and the biochemical analysis unit and thestimulable phosphor sheet are superposed on each other so that each ofthe plurality of dot-like stimulable phosphor layer regions faces one ofthe absorptive regions in the plurality of holes formed in the substrateof the biochemical analysis unit, thereby exposing the plurality ofdot-like stimulable phosphor layer regions of the stimulable phosphorsheet to the radioactive labeling substance contained in the pluralityof absorptive regions. It is therefore possible to reliably preventelectron beams released from the radioactive labeling substancecontained in the individual absorptive regions from being scattered andadvancing to the dot-like stimulable phosphor layer regions facingneighboring absorptive regions. Therefore, the plurality of dot-likestimulable phosphor layer regions formed in the stimulable phosphorsheet can be reliably exposed to the radioactive labeling substancecontained in corresponding absorptive regions, thereby improving thequantitative accuracy of biochemical analysis.

[0045] In a preferred aspect of the present invention, the substrate ofthe biochemical analysis unit is made of a material capable ofattenuating radiation energy and light energy, and the biochemicalanalysis is effected based on biochemical analysis data produced by thesteps of preparing the biochemical analysis unit by specifically bindinga substance derived from a living organism and labeled with afluorescent substance, in addition to a radioactive labeling substance,with the specific binding substances, thereby selectively labeling theplurality of absorptive regions, irradiating the biochemical analysisunit with a stimulating ray, thereby stimulating the fluorescentsubstance, and photoelectrically detecting fluorescence released fromthe fluorescent substance.

[0046] According to this preferred aspect of the present invention, thesubstrate of the biochemical analysis unit is made of a material capableof attenuating radiation energy and light energy, and the biochemicalanalysis is effected based on biochemical analysis data produced by thesteps of preparing the biochemical analysis unit by specifically bindinga substance derived from a living organism and labeled with afluorescent substance, in addition to a radioactive labeling substance,with the specific binding substances, thereby selectively labeling theplurality of absorptive regions, irradiating the biochemical analysisunit with a stimulating ray, thereby stimulating the fluorescentsubstance, and photoelectrically detecting fluorescence released fromthe fluorescent substance. A specimen can therefore be labeled with afluorescent substance in addition to a radioactive labeling substanceand, therefore, the utility of biochemical analysis can be improved.

[0047] In a preferred aspect of the present invention, the substrate ofthe biochemical analysis unit is made of a material capable ofattenuating radiation energy and light energy, and the biochemicalanalysis is effected based on biochemical analysis data produced by thesteps of preparing the biochemical analysis unit by specifically bindinga substance derived from a living organism and labeled with a labelingsubstance which generates chemiluminescent emission when it contacts achemiluminescent substrate, in addition to a radioactive labelingsubstance, with the specific binding substances, thereby selectivelylabeling the plurality of absorptive regions, bringing the biochemicalanalysis unit into contact with a chemiluminescent substrate, andphotoelectrically detecting chemiluminescent emission released from thelabeling substance.

[0048] According to this preferred aspect of the present invention, thesubstrate of the biochemical analysis unit is made of a material capableof attenuating radiation energy and light energy, and the biochemicalanalysis is effected based on biochemical analysis data produced by thesteps of preparing the biochemical analysis unit by specifically bindinga substance derived from a living organism and labeled with a labelingsubstance which generates chemiluminescent emission when it contacts achemiluminescent substrate, in addition to a radioactive labelingsubstance, with the specific binding substances, thereby selectivelylabeling the plurality of absorptive regions, bringing the biochemicalanalysis unit into contact with a chemiluminescent substrate, andphotoelectrically detecting chemiluminescent emission released from thelabeling substance. A specimen can therefore be labeled with a labelingsubstance capable of generating chemiluminescent emission when itcontacts a chemiluminescent substrate in addition to a radioactivelabeling substance and, therefore, the utility of biochemical analysiscan be improved.

[0049] In a preferred aspect of the present invention, the substrate ofthe biochemical analysis unit is made of a material capable ofattenuating radiation energy and light energy, and the biochemicalanalysis is effected based on biochemical analysis data produced by thesteps of preparing the biochemical analysis unit by specifically bindinga substance derived from a living organism and labeled with, in additionto a radioactive labeling substance, a fluorescent substance and alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate with the specific bindingsubstances, thereby selectively labeling the plurality of absorptiveregions, irradiating the biochemical analysis unit with a stimulatingray to stimulate the fluorescent substance, and photoelectricallydetecting fluorescence released from the fluorescent substance, whilebringing the biochemical analysis unit into contact with achemiluminescent substrate, and photoelectrically detectingchemiluminescent emission released from the labeling substance.

[0050] According to this preferred aspect of the present invention, thesubstrate of the biochemical analysis unit is made of a material capableof attenuating radiation energy and light energy, and the biochemicalanalysis is effected based on biochemical analysis data produced by thesteps of preparing the biochemical analysis unit by specifically bindinga substance derived from a living organism and labeled with, in additionto a radioactive labeling substance, a fluorescent substance and alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate with the specific bindingsubstances, thereby selectively labeling the plurality of absorptiveregions, irradiating the biochemical analysis unit with a stimulatingray to stimulate the fluorescent substance, and photoelectricallydetecting fluorescence released from the fluorescent substance, whilebringing the biochemical analysis unit into contact with achemiluminescent substrate, and photoelectrically detectingchemiluminescent emission released from the labeling substance. Aspecimen can therefore be labeled with a fluorescent substance and alabeling substance capable of generating chemiluminescent emission whenit contacts a chemiluminescent substrate, in addition to a radioactivelabeling substance, and, therefore, the utility of biochemical analysiscan be improved.

[0051] The above and other objects of the present invention can be alsoaccomplished by a biochemical analyzing method comprising the steps ofpreparing a biochemical analysis unit comprising an absorptive substrateformed of an absorptive material and a perforated plate made of amaterial capable of attenuating radiation energy and light energy andformed with a plurality of through-holes, the perforated plate beingclosely contacted with at least one surface of the absorptive substrateto form a plurality of absorptive regions of the absorptive substrate inthe plurality of through-holes formed in the perforated plate, theplurality of absorptive regions being selectively labeled with aradioactive labeling substance by spotting specific binding substances,which can specifically bind with a substance derived from a livingorganism and whose sequence, base length, composition and the like areknown, in the plurality of absorptive regions and specifically binding asubstance derived from a living organism and labeled with a radioactivelabeling substance, superposing the biochemical analysis unit and astimulable phosphor sheet in which a stimulable phosphor layer is formedvia the perforated plate so that the stimulable phosphor layer faces theplurality of absorptive regions, thereby exposing the stimulablephosphor layer to the radioactive labeling substance contained in theplurality of absorptive regions, irradiating the stimulable phosphorlayer exposed to the radioactive labeling substance with a stimulatingray to excite stimulable phosphor contained in the stimulable phosphorlayer, photoelectrically detecting stimulated emission released from thestimulable phosphor contained in the stimulable phosphor layer toproduce biochemical analysis data, and effecting biochemical analysisbased on the biochemical analysis data.

[0052] According to this aspect of the present invention, a biochemicalanalyzing method comprises the steps of preparing a biochemical analysisunit comprising an absorptive substrate formed of an absorptive materialand a perforated plate made of a material capable of attenuatingradiation energy and light energy and formed with a plurality ofthrough-holes, the perforated plate being closely contacted with atleast one surface of the absorptive substrate so that a plurality ofabsorptive regions are formed of the absorptive substrate in theplurality of the through-holes formed in the perforated plate, theplurality of absorptive regions being selectively labeled with aradioactive labeling substance by spotting specific binding substances,which can specifically bind with a substance derived from a livingorganism and whose sequence, base length, composition and the like areknown, in the plurality of absorptive regions and specifically binding asubstance derived from a living organism and labeled with a radioactivelabeling substance, superposing the biochemical analysis unit and astimulable phosphor sheet in which a stimulable phosphor layer is formedvia the perforated plate so that the stimulable phosphor layer faces theplurality of absorptive regions, thereby exposing the stimulablephosphor layer to the radioactive labeling substance contained in theplurality of absorptive regions, irradiating the stimulable phosphorlayer exposed to the radioactive labeling substance with a stimulatingray to excite stimulable phosphor contained in the stimulable phosphorlayer, photoelectrically detecting stimulated emission released from thestimulable phosphor contained in the stimulable phosphor layer toproduce biochemical analysis data, and effecting biochemical analysisbased on the biochemical analysis data. Therefore, since electron beams(β rays) released from the radioactive labeling substance contained inthe individual absorptive regions and electron beams released fromneighboring absorptive regions can be reliably separated by theperforated plate, thereby reliably preventing electron beams releasedfrom the radioactive labeling substance contained in the individualabsorptive regions from advancing to regions of the stimulable phosphorlayer that should be exposed to electron beams released from neighboringabsorptive regions, it is possible to efficiently prevent noise causedby the scattering of electron beams released from the radioactivelabeling substance from being generated in biochemical analysis dataproduced by irradiating the stimulable phosphor layer exposed to theradioactive labeling substance with a stimulating ray andphotoelectrically detecting stimulated emission released from thestimulable phosphor layer and produce biochemical analysis data havinghigh quantitative accuracy.

[0053] In a preferred aspect of the present invention, perforated platesare closely contacted with both surfaces of the absorptive substrate,thereby forming the biochemical analysis unit, and biochemical analysisdata are produced by superposing the biochemical analysis unit and thestimulable phosphor sheet via one of the perforated plates so that thestimulable phosphor layer faces the plurality of absorptive regions andexposing the stimulable phosphor layer to a radioactive labelingsubstance contained in the plurality of absorptive regions.

[0054] In a preferred aspect of the present invention, the specificbinding substances are spotted through the plurality of through-holes inthe plurality of absorptive regions formed on the absorptive substrate.

[0055] In a preferred aspect of the present invention, a plurality ofdot-like stimulable phosphor layer regions are formed spaced-apart inthe stimulable phosphor sheet in the same pattern as that of theplurality of through-holes formed in the perforated plate, and thebiochemical analysis unit and the stimulable phosphor sheet aresuperposed on each other so that each of the plurality of dot-likestimulable phosphor layer regions faces one of the plurality ofabsorptive regions via one of the through-holes formed in the perforatedplate, thereby exposing the plurality of dot-like stimulable phosphorlayer regions to a radioactive labeling substance contained in theplurality of absorptive regions.

[0056] According to this preferred aspect of the present invention, aplurality of dot-like stimulable phosphor layer regions are formedspaced-apart in the stimulable phosphor sheet in the same pattern asthat of the plurality of through-holes formed in the perforated plate,and the biochemical analysis unit and the stimulable phosphor sheet aresuperposed on each other so that each of the plurality of dot-likestimulable phosphor layer regions faces one of the plurality ofabsorptive regions via one of the through-holes formed in the perforatedplate, thereby exposing the plurality of dot-like stimulable phosphorlayer regions to a radioactive labeling substance contained in theplurality of absorptive regions. Electron beams (β rays) released from aradioactive labeling substance contained in the individual absorptiveregions are therefore prevented from being scattered in the substrateand scattered electron beams are prevented from advancing to thedot-like stimulable phosphor layer regions facing neighboring absorptiveregions. Therefore, the plurality of dot-like stimulable phosphor layerregions formed in the stimulable phosphor sheet can be reliably exposedto the radioactive labeling substance contained in correspondingabsorptive regions, thereby improving the quantitative accuracy ofbiochemical analysis.

[0057] In a preferred aspect of the present invention, the perforatedplate is made of a material capable of attenuating radiation energy andlight energy, and the biochemical analysis is effected based onbiochemical analysis data produced by the steps of preparing thebiochemical analysis unit by specifically binding a substance derivedfrom a living organism and labeled with a fluorescent substance, inaddition to a radioactive labeling substance, with the specific bindingsubstances, thereby selectively labeling the plurality of absorptiveregions; irradiating the biochemical analysis unit with a stimulatingray through the plurality of the through-holes formed in the perforatedplate, thereby stimulating the fluorescent substance, andphotoelectrically detecting fluorescence released from the fluorescentsubstance.

[0058] According to this preferred aspect of the present invention, theperforated plate is made of a material capable of attenuating radiationenergy and light energy, and the biochemical analysis is effected basedon biochemical analysis data produced by the steps of preparing thebiochemical analysis unit by specifically binding a substance derivedfrom a living organism and labeled with a fluorescent substance, inaddition to a radioactive labeling substance, with the specific bindingsubstances, thereby selectively labeling the plurality of absorptiveregions, irradiating the biochemical analysis unit with a stimulatingray through the plurality of the through-holes formed in the perforatedplate, thereby stimulating the fluorescent substance, andphotoelectrically detecting fluorescence released from the fluorescentsubstance. A specimen can therefore be labeled with a fluorescentsubstance in addition to a radioactive labeling substance and,therefore, the utility of biochemical analysis can be improved.

[0059] In a preferred aspect of the present invention, the perforatedplate is made of a material capable of attenuating radiation energy andlight energy, and the biochemical analysis is effected based onbiochemical analysis data produced by the steps of preparing thebiochemical analysis unit by specifically binding a substance derivedfrom a living organism and labeled with, in addition to a radioactivelabeling substance, a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substratewith the specific binding substances, thereby selectively labeling theplurality of absorptive regions, bringing the biochemical analysis unitinto close contact with a chemiluminescent substrate through theplurality of the through-holes formed in the perforated plate, andphotoelectrically detecting chemiluminescent emission released from thelabeling substance.

[0060] According to this preferred aspect of the present invention, thesubstrate of the biochemical analysis unit is made of a material capableof attenuating radiation energy and light energy, and the biochemicalanalysis is effected based on biochemical analysis data produced by thesteps of preparing the biochemical analysis unit by specifically bindinga substance derived from a living organism and labeled with, in additionto a radioactive labeling substance, a labeling substance whichgenerates chemiluminescent emission when it contacts a chemiluminescentsubstrate with the specific binding substances, thereby selectivelylabeling the plurality of absorptive regions, bringing the biochemicalanalysis unit into close contact with a chemiluminescent substratethrough the plurality of the through-holes formed in the perforatedplate, and photoelectrically detecting chemiluminescent emissionreleased from the labeling substance. A specimen can therefore belabeled with a labeling substance capable of generating chemiluminescentemission when it contacts a chemiluminescent substrate, in addition to aradioactive labeling substance, and, therefore, the utility ofbiochemical analysis can be improved.

[0061] In a preferred aspect of the present invention, the perforatedplate is made of a material capable of attenuating radiation energy andlight energy, and the biochemical analysis is effected based onbiochemical analysis data produced by the steps of preparing thebiochemical analysis unit by specifically binding a substance derivedfrom a living organism and labeled with, in addition to a radioactivelabeling substance, a fluorescent substance and a labeling substancewhich generates chemiluminescent emission when it contacts achemiluminescent substrate with the specific binding substances, therebyselectively labeling the plurality of absorptive regions, irradiatingthe biochemical analysis unit with a stimulating ray through theplurality of the through-holes formed in the perforated plate tostimulate the fluorescent substance, and photoelectrically detectingfluorescence released from the fluorescent substance, while bringing thebiochemical analysis unit into close contact with a chemiluminescentsubstrate through the plurality of the through-holes formed in theperforated plate, and photoelectrically detecting chemiluminescentemission released from the labeling substance.

[0062] According to this preferred aspect of the present invention, theperforated plate is made of a material capable of attenuating radiationenergy and light energy, and the biochemical analysis is effected basedon biochemical analysis data produced by the steps of preparing thebiochemical analysis unit by specifically binding a substance derivedfrom a living organism and labeled with, in addition to a radioactivelabeling substance, a fluorescent substance and a labeling substancewhich generates chemiluminescent emission when it contacts achemiluminescent substrate with the specific binding substances, therebyselectively labeling the plurality of absorptive regions, irradiatingthe biochemical analysis unit with a stimulating ray through theplurality of the through-holes formed in the perforated plate tostimulate the fluorescent substance, and photoelectrically detectingfluorescence released from the fluorescent substance, while bringing thebiochemical analysis unit into close contact with a chemiluminescentsubstrate through the plurality of the through-holes formed in theperforated plate, and photoelectrically detecting chemiluminescentemission released from the labeling substance. A specimen can thereforebe labeled with a fluorescent substance and a labeling substance capableof generating chemiluminescent emission when it contacts achemiluminescent substrate, in addition to a radioactive labelingsubstance, and, therefore, the utility of biochemical analysis can beimproved.

[0063] The above and other objects of the present invention can also beaccomplished by a biochemical analyzing method comprising the steps ofpreparing a biochemical analysis unit by spotting specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, in a plurality of absorptive regions formed in aplurality of holes formed in a substrate made of a material capable ofattenuating light energy and specifically binding a substance derivedfrom a living organism and labeled with a fluorescent substance with thespecific binding substances, thereby selectively labeling a plurality ofabsorptive regions, irradiating the biochemical analysis unit with astimulating ray, thereby exciting the fluorescent substance,photoelectrically detecting fluorescence released from the fluorescentsubstance, thereby producing biochemical analysis data, and effectingbiochemical analysis based on the biochemical analysis data.

[0064] According to this aspect of the present invention, thebiochemical analysis unit is prepared by spotting specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, in a plurality of absorptive regions formed in aplurality of holes formed in a substrate made of a material capable ofattenuating radiation energy and specifically binding a substancederived from a living organism and labeled with a fluorescent substancewith the specific binding substances, thereby selectively labeling aplurality of absorptive regions. Biochemical analysis data are thenproduced by irradiating the biochemical analysis unit with a stimulatingray to stimulate the fluorescent substance and photoelectricallydetecting fluorescence released from the fluorescent substance andbiochemical analysis is effected based on the biochemical analysis data.Therefore, when the biochemical data are produced by irradiating thebiochemical analysis unit with a stimulating ray and photoelectricallydetecting fluorescence released from the fluorescent substance, sincefluorescence is reliably prevented from being scattered in the substrateof the biochemical analysis unit, it is possible to efficiently preventnoise caused by the scattering of fluorescence from being generated inbiochemical analysis data produced by photoelectrically detectingfluorescence and to effect biochemical analysis with high quantitativeaccuracy.

[0065] In a preferred aspect of the present invention, the plurality ofabsorptive regions are formed by charging an absorptive material in theplurality of holes formed in the substrate of the biochemical analysisunit.

[0066] In a preferred aspect of the present invention, the plurality ofholes formed in the substrate of the biochemical analysis unit areconstituted as through-holes.

[0067] In another preferred aspect of the present invention, theplurality of holes formed in the substrate of the biochemical analysisunit are constituted as recesses.

[0068] The above and other objects of the present invention can also beaccomplished by a biochemical analyzing method comprising the steps ofpreparing a biochemical analysis unit by spotting specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, in a plurality of absorptive regions formed in aplurality of holes formed in a substrate made of a material capable ofattenuating light energy and specifically binding a substance derivedfrom a living organism and labeled with a labeling substance capable ofgenerating chemiluminescent emission when it contacts a chemiluminescentsubstrate with the specific binding substances, thereby selectivelylabeling the plurality of absorptive regions, bringing the biochemicalanalysis unit into close contact with a chemiluminescent substrate,photoelectrically detecting chemiluminescent emission released from thelabeling substance, thereby producing biochemical analysis data, andeffecting biochemical analysis based on the biochemical analysis data.

[0069] According to this aspect of the present invention, thebiochemical analysis unit is prepared by spotting specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, in a plurality of absorptive regions formed in aplurality of holes formed in a substrate made of a material capable ofattenuating radiation energy and specifically binding a substancederived from a living organism and labeled with a labeling substancecapable of generating chemiluminescent emission when it contacts achemiluminescent substrate with the specific binding substances, therebyselectively labeling the plurality of absorptive regions. Biochemicalanalysis data are the produced by bringing the biochemical analysis unitinto close contact with a chemiluminescent substrate andphotoelectrically detecting chemiluminescent emission released from thelabeling substance and biochemical analysis is effected based on thebiochemical analysis data. Therefore, when the biochemical data areproduced by bringing the biochemical analysis unit into close contactwith a chemiluminescent substrate and photoelectrically detectingchemiluminescent emission released from the labeling substance, sincechemiluminescent emission is reliably prevented from being scattered inthe substrate of the biochemical analysis unit, it is possible toefficiently prevent noise caused by the scattering of chemiluminescentemission from being generated in biochemical analysis data produced byphotoelectrically detecting chemiluminescent emission and effectbiochemical analysis with high quantitative accuracy.

[0070] In a preferred aspect of the present invention, the plurality ofabsorptive regions are formed by charging an absorptive material in theplurality of holes formed in the substrate of the biochemical analysisunit.

[0071] In a preferred aspect of the present invention, the plurality ofholes formed in the substrate of the biochemical analysis unit areconstituted as through-holes.

[0072] In another preferred aspect of the present invention, theplurality of holes formed in the substrate of the biochemical analysisunit are constituted as recesses.

[0073] The above and other objects of the present invention can also beaccomplished by a biochemical analyzing method comprising the steps ofpreparing a biochemical analysis unit by spotting specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, in a plurality of absorptive regions formed in aplurality of holes formed in a substrate made of a material capable ofattenuating light energy and specifically binding a substance derivedfrom a living organism and labeled with a fluorescent substance and alabeling substance capable of generating chemiluminescent emission whenit contacts a chemiluminescent substrate with the specific bindingsubstances, thereby selectively labeling the plurality of absorptiveregions, irradiating the biochemical analysis unit with a stimulatingray to excite the fluorescent substance, and photoelectrically detectingfluorescence released from the fluorescent substance, thereby producingbiochemical analysis data, while bringing the biochemical analysis unitinto close contact with a chemiluminescent substrate, photoelectricallydetecting chemiluminescent emission released from the labelingsubstance, thereby producing biochemical analysis data, and effectingbiochemical analysis based on the biochemical analysis data.

[0074] According to this aspect of the present invention, thebiochemical analysis unit is prepared by spotting specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, in a plurality of absorptive regions formed in aplurality of holes formed in a substrate made of a material capable ofattenuating radiation energy and specifically binding a substancederived from a living organism and labeled with a fluorescent substanceand a labeling substance capable of generating chemiluminescent emissionwhen it contacts a chemiluminescent substrate with the specific bindingsubstances, thereby selectively labeling the plurality of absorptiveregions. Biochemical analysis data are then produced by irradiating thebiochemical analysis unit with a stimulating ray to stimulate thefluorescent substance and photoelectrically detecting fluorescencereleased from the fluorescent substance and are also produced bybringing the biochemical analysis unit into close contact with achemiluminescent substrate and photoelectrically detectingchemiluminescent emission released from the labeling substance, andbiochemical analysis is effected based on the biochemical analysis data.Therefore, when the biochemical data are produced by reading fluorescentdata, since fluorescence is reliably prevented from being scattered inthe substrate of the biochemical analysis unit, it is possible toefficiently prevent noise caused by the scattering of fluorescence frombeing generated in biochemical analysis data produced byphotoelectrically detecting fluorescence. On the other hand, when thebiochemical data are produced by reading chemiluminescent data, sincechemiluminescent emission is reliably prevented from being scattered inthe substrate of the biochemical analysis unit, it is possible toefficiently prevent noise caused by the scattering of chemiluminescentemission from being generated in biochemical analysis data produced byphotoelectrically detecting chemiluminescent emission. Therefore,biochemical analysis can be effected with high quantitative accuracy.

[0075] In a preferred aspect of the present invention, the plurality ofabsorptive regions are formed by charging an absorptive material in theplurality of holes formed in the substrate of the biochemical analysisunit.

[0076] In a preferred aspect of the present invention, the plurality ofholes formed in the substrate of the biochemical analysis unit areconstituted as through-holes.

[0077] In another preferred aspect of the present invention, theplurality of holes formed in the substrate of the biochemical analysisunit are constituted as recesses.

[0078] The above and other objects of the present invention can also beaccomplished by a biochemical analyzing method comprising the steps ofbringing an absorptive substrate made of an absorptive material andformed with a plurality of absorptive regions by spotting thereonspecific binding substances, which can specifically bind with asubstance derived from a living organism and whose sequence, baselength, composition and the like are known, the plurality of theabsorptive regions being selectively labeled by specifically binding asubstance derived from a living organism and labeled with a fluorescentsubstance with the specific binding substances contained in theplurality of absorptive regions, into close contact with a perforatedplate made of a material capable of attenuating light energy and formedwith a plurality of through-holes at positions corresponding to theplurality of absorptive regions formed in the absorptive substrate,irradiating the plurality of absorptive regions formed in the absorptivesubstrate through the plurality of through-holes formed in theperforated plate to stimulate the fluorescent substance,photoelectrically detecting fluorescence released from the fluorescentsubstance, thereby producing biochemical analysis data, and effectingbiochemical analysis based on the biochemical analysis data.

[0079] According to this aspect of the present invention, a biochemicalanalyzing method comprises the steps of bringing an absorptive substratemade of an absorptive material and formed with a plurality of absorptiveregions by spotting thereon specific binding substances, which canspecifically bind with a substance derived from a living organism andwhose sequence, base length, composition and the like are known, ontothe absorptive substrate, the plurality of the absorptive regions beingselectively labeled by specifically binding a substance derived from aliving organism and labeled with a fluorescent substance with thespecific binding substances contained in the plurality of absorptiveregions, into close contact with a perforated plate made of a materialcapable of attenuating light energy and formed with a plurality ofthrough-holes at positions corresponding to the plurality of absorptiveregions formed in the absorptive substrate, irradiating the plurality ofabsorptive regions formed in the absorptive substrate through theplurality of through-holes formed in the perforated plate to stimulatethe fluorescent substance, photoelectrically detecting fluorescencereleased from the fluorescent substance, thereby producing biochemicalanalysis data, and effecting biochemical analysis based on thebiochemical analysis data. Therefore, when biochemical analysis data areproduced by irradiating the plurality of absorptive regions formed inthe absorptive substrate with a stimulating ray through the plurality ofthrough-holes formed in the perforated plate and photoelectricallydetecting fluorescence released from the fluorescent substance, sincefluorescence released from each of the plurality of absorptive regionscan be reliably separated by the perforated plate from fluorescencereleased from neighboring absorptive regions, it is possible toefficiently prevent noise caused by the scattering of fluorescence frombeing generated in biochemical analysis data produced byphotoelectrically detecting fluorescence and effect biochemical analysiswith high quantitative accuracy.

[0080] In a preferred aspect of the present invention, the biochemicalanalysis unit is prepared by bringing perforated plates into closecontact with both surfaces of the absorptive substrate and biochemicaldata are produced by irradiating the plurality of absorptive regionsformed in the absorptive substrate with a stimulating ray through theplurality of through-holes formed in one of the perforated plates tostimulate a fluorescent substance and photoelectrically detectingfluorescence released from the fluorescent substance.

[0081] In a preferred aspect of the present invention, the specificbinding substances are spotted through the plurality of through-holesformed in the perforated plate in the plurality of absorptive regionsformed in the absorptive substrate.

[0082] The above and other objects of the present invention can also beaccomplished by a biochemical analyzing method comprising the steps ofbringing an absorptive substrate made of an absorptive material andformed with a plurality of absorptive regions by spotting thereonspecific binding substances, which can specifically bind with asubstance derived from a living organism and whose sequence, baselength, composition and the like are known, the plurality of theabsorptive regions being selectively labeled by specifically binding asubstance derived from a living organism and labeled with a labelingsubstance capable of generating chemiluminescent emission when itcontacts a chemiluminescent substrate with the specific bindingsubstances contained in the plurality of absorptive regions, into closecontact with a perforated plate made of a material capable ofattenuating light energy and formed with a plurality of through-holes atpositions corresponding to the plurality of absorptive regions formed inthe absorptive substrate, bringing a chemiluminescent substrate intoclose contact with the plurality of absorptive regions formed in theabsorptive substrate through the plurality of through-holes formed inthe perforated plate, photoelectrically detecting chemiluminescentemission released from the labeling substance, thereby producingbiochemical analysis data, and effecting biochemical analysis based onthe biochemical analysis data.

[0083] According to this aspect of the present invention, a biochemicalanalyzing method comprises the steps of bringing an absorptive substratemade of an absorptive material and formed with a plurality of absorptiveregions by spotting thereon specific binding substances, which canspecifically bind with a substance derived from a living organism andwhose sequence, base length, composition and the like are known, ontothe absorptive substrate, the plurality of the absorptive regions beingselectively labeled by specifically binding a substance derived from aliving organism and labeled with a labeling substance capable ofgenerating chemiluminescent emission when it contacts a chemiluminescentsubstrate with the specific binding substances contained in theplurality of absorptive regions, into close contact with a perforatedplate made of a material capable of attenuating light energy and formedwith a plurality of through-holes at positions corresponding to theplurality of absorptive regions formed in the absorptive substrate,bringing a chemiluminescent substrate into close contact with theplurality of absorptive regions formed in the absorptive substratethrough the plurality of through-holes formed in the perforated plate,photoelectrically detecting chemiluminescent emission released from thelabeling substance, thereby producing biochemical analysis data, andeffecting biochemical analysis based on the biochemical analysis data.Therefore, when biochemical analysis data are produced by bringing achemiluminescent substrate into close contact with the plurality ofabsorptive regions formed in the absorptive substrate through theplurality of through-holes formed in the perforated plate andphotoelectrically detecting chemiluminescent emission released from thelabeling substance, since chemiluminescent emission released from eachof the plurality of absorptive regions can be reliably separated by theperforated plate from chemiluminescent emission released fromneighboring absorptive regions, it is possible to efficiently preventnoise caused by the scattering of chemiluminescent emission from beinggenerated in biochemical analysis data produced by photoelectricallydetecting chemiluminescent emission and effect biochemical analysis withhigh quantitative accuracy.

[0084] In a preferred aspect of the present invention, the biochemicalanalysis unit is prepared by bringing perforated plates into closecontact with the both surfaces of the absorptive substrate andbiochemical data are produced by bringing a chemiluminescent substrateinto close contact with the plurality of absorptive regions formed inthe absorptive substrate through the plurality of through-holes formedin one of the perforated plates and photoelectrically detectingchemiluminescent emission released from the labeling substance.

[0085] In a preferred aspect of the present invention, the specificbinding substances are spotted through the plurality of through-holesformed in the perforated plate in the plurality of absorptive regionsformed in the absorptive substrate.

[0086] The above and other objects of the present invention can also beaccomplished by a biochemical analyzing method comprising the steps ofbringing an absorptive substrate made of an absorptive material andformed with a plurality of absorptive regions by spotting thereonspecific binding substances, which can specifically bind with asubstance derived from a living organism and whose sequence, baselength, composition and the like are known, the plurality of theabsorptive regions being selectively labeled by specifically binding asubstance derived from a living organism and labeled with a fluorescentsubstance and a labeling substance capable of generatingchemiluminescent emission when it contacts a chemiluminescent substratewith the specific binding substances contained in the plurality ofabsorptive regions, into close contact with a perforated plate made of amaterial capable of attenuating light energy and formed with a pluralityof through-holes at positions corresponding to the plurality ofabsorptive regions formed in the absorptive substrate, irradiating theplurality of absorptive regions formed in the absorptive substratethrough the plurality of through-holes formed in the perforated plate tostimulate the fluorescent substance, and photoelectrically detectingfluorescence released from the fluorescent substance, thereby producingbiochemical analysis data, while bringing a chemiluminescent substrateinto close contact with the plurality of absorptive regions formed inthe absorptive substrate through the plurality of through-holes formedin the perforated plate, and photoelectrically detectingchemiluminescent emission released from the labeling substance, therebyproducing biochemical analysis data, and effecting biochemical analysisbased on the biochemical analysis data.

[0087] According to this aspect the present invention, a biochemicalanalyzing method comprises the steps of bringing an absorptive substratemade of an absorptive material and formed with a plurality of absorptiveregions by spotting thereon specific binding substances, which canspecifically bind with a substance derived from a living organism andwhose sequence, base length, composition and the like are known, theplurality of the absorptive regions being selectively labeled byspecifically binding a substance derived from a living organism andlabeled with a fluorescent substance and a labeling substance capable ofgenerating chemiluminescent emission when it contacts a chemiluminescentsubstrate with the specific binding substances contained in theplurality of absorptive regions, into close contact with a perforatedplate made of a material capable of attenuating light energy and formedwith a plurality of through-holes at positions corresponding to theplurality of absorptive regions formed in the absorptive substrate,irradiating the plurality of absorptive regions formed in the absorptivesubstrate through the plurality of through-holes formed in theperforated plate to stimulate the fluorescent substance, andphotoelectrically detecting fluorescence released from the fluorescentsubstance, thereby producing biochemical analysis data, while bringing achemiluminescent substrate into close contact with the plurality ofabsorptive regions formed in the absorptive substrate through theplurality of through-holes formed in the perforated plate, andphotoelectrically detecting chemiluminescent emission released from thelabeling substance, thereby producing biochemical analysis data, andeffecting biochemical analysis based on the biochemical analysis data.Therefore, when biochemical analysis data are produced by irradiatingthe plurality of absorptive regions formed in the absorptive substratewith a stimulating ray through the plurality of through-holes formed inthe perforated plate and photoelectrically detecting fluorescencereleased from the fluorescent substance, since fluorescence releasedfrom each of the plurality of absorptive regions can be reliablyseparated by the perforated plate from fluorescence released fromneighboring absorptive regions, it is possible to efficiently preventnoise caused by the scattering of fluorescence from being generated inbiochemical analysis data produced by photoelectrically detectingfluorescence. On the other hand, when biochemical analysis data areproduced by bringing a chemiluminescent substrate into close contactwith the plurality of absorptive regions formed in the absorptivesubstrate through the plurality of through-holes formed in theperforated plate and photoelectrically detecting chemiluminescentemission released from the labeling substance, since chemiluminescentemission released from each of the plurality of absorptive regions canbe reliably separated by the perforated plate from chemiluminescentemission released from neighboring absorptive regions, it is possible toefficiently prevent noise caused by the scattering of chemiluminescentemission from being generated in biochemical analysis data produced byphotoelectrically detecting chemiluminescent emission. Therefore,biochemical analysis can be effected with high quantitative accuracy.

[0088] In a preferred aspect of the present invention, the biochemicalanalysis unit is prepared by bringing perforated plates into closecontact with the both surfaces of the absorptive substrate andbiochemical data are produced by irradiating the plurality of absorptiveregions formed in the absorptive substrate with a stimulating raythrough the plurality of through-holes formed in one of the perforatedplates to stimulate a fluorescent substance and photoelectricallydetecting fluorescence released from the fluorescent substance and arealso produced by bringing a chemiluminescent substrate into closecontact with the plurality of absorptive regions formed in theabsorptive substrate through the plurality of through-holes formed inone of the perforated plates and photoelectrically detectingchemiluminescent emission released from the labeling substance.

[0089] In a preferred aspect of the present invention, the specificbinding substances are spotted through the plurality of through-holesformed in the perforated plate in the plurality of absorptive regionsformed in the absorptive substrate.

[0090] In a preferred aspect of the present invention, the substancederived from a living organism is specifically bound with specificbinding substances by a reaction selected from a group consisting ofhybridization, antigen-antibody reaction and receptor-ligand reaction.

[0091] In a preferred aspect of the present invention, the materialcapable of attenuating radiation energy has a property of reducing theenergy of radiation to ⅕ or less when the radiation travels in thematerial by a distance equal to that between neighboring absorptiveregions.

[0092] In a further preferred aspect of the present invention, amaterial capable of attenuating radiation energy has a property ofreducing the energy of radiation to {fraction (1/10)} or less when theradiation travels in the material by a distance equal to that betweenneighboring absorptive regions.

[0093] In a further preferred aspect of the present invention, amaterial capable of attenuating radiation energy has a property ofreducing the energy of radiation to {fraction (1/50)} or less when theradiation travels in the material by a distance equal to that betweenneighboring absorptive regions.

[0094] In a further preferred aspect of the present invention, amaterial capable of attenuating radiation energy has a property ofreducing the energy of radiation to {fraction (1/100)} or less when theradiation travels in the material by a distance equal to that betweenneighboring absorptive regions.

[0095] In a further preferred aspect of the present invention, amaterial capable of attenuating radiation energy has property ofreducing the energy of radiation to {fraction (1/500)} or less when theradiation travels in the material by a distance equal to that betweenneighboring absorptive regions.

[0096] In a further preferred aspect of the present invention, amaterial capable of attenuating radiation energy has a property ofreducing the energy of radiation to {fraction (1/1000)} or less when theradiation travels in the material by a distance equal to that betweenneighboring absorptive regions.

[0097] In a preferred aspect of the present invention, a materialcapable of attenuating light energy has a property of reducing theenergy of light to ⅕ or less when the light travels in the material by adistance equal to that between neighboring absorptive regions.

[0098] In a further preferred aspect of the present invention, amaterial capable of attenuating light energy has a property of reducingthe energy of light to {fraction (1/10)} or less when the light travelsin the material by a distance equal to that between neighboringabsorptive regions.

[0099] In a further preferred aspect of the present invention, amaterial capable of attenuating light energy has a property of reducingthe energy of light to {fraction (1/50)} or less when the light travelsin the material by a distance equal to that between neighboringabsorptive regions.

[0100] In a further preferred aspect of the present invention, amaterial capable of attenuating light energy has a property of reducingthe energy of light to {fraction (1/100)} or less when the light travelsin the material by a distance equal to that between neighboringabsorptive regions.

[0101] In a further preferred aspect of the present invention, amaterial capable of attenuating light energy has a property of reducingthe energy of light to {fraction (1/500)} or less when the light travelsin the material by a distance equal to that between neighboringabsorptive regions.

[0102] In a further preferred aspect of the present invention, amaterial capable of attenuating light energy has a property of reducingthe energy of light to {fraction (1/1000)} or less when the lighttravels in the material by a distance equal to that between neighboringabsorptive regions.

[0103] In the present invention, the material for forming the substrateor the perforated plate of the biochemical analysis unit is notparticularly limited but may be of any type of inorganic compoundmaterial or organic compound material insofar as it can attenuateradiation energy and/or light energy. It is preferably formed of metalmaterial, ceramic material or plastic material.

[0104] In the present invention, illustrative examples of inorganiccompound materials capable of attenuating radiation energy andpreferably usable for forming a substrate or a perforated plate of abiochemical analysis unit in the present invention include metals suchas gold, silver, copper, zinc, aluminum, titanium, tantalum, chromium,iron, nickel, cobalt, lead, tin, selenium and the like; alloys such asbrass, stainless steel, bronze and the like; silicon materials such assilicon, amorphous silicon, glass, quartz, silicon carbide, siliconnitride and the like; metal oxides such as aluminum oxide, magnesiumoxide, zirconium oxide and the like; and inorganic salts such astungsten carbide, calcium carbide, calcium sulfate, hydroxy apatite,gallium arsenide and the like. These may have either a monocrystalstructure or a polycrystal sintered structure such as amorphous, ceramicor the like.

[0105] In the present invention, a high molecular compound is preferablyused as an organic compound material capable of attenuating radiationenergy. Illustrative examples of high molecular compounds preferablyusable for forming the substrate or the perforated plate of thebiochemical analysis unit in the present invention include polyolefinssuch as polyethylene, polypropylene and the like; acrylic resins such aspolymethyl methacrylate, polybutylacrylate/polymethyl methacrylatecopolymer and the like; polyacrylonitrile; polyvinyl chloride;polyvinylidene chloride; polyvinylidene fluoride;polytetrafluoroethylene; polychlorotrifuluoroethylene; polycarbonate;polyesters such as polyethylene naphthalate, polyethylene terephthalateand the like; nylons such as nylon-6, nylon-6, 6, nylon-4, 10 and thelike; polyimide; polysulfone; polyphenylene sulfide; silicon resins suchas polydiphenyl siloxane and the like; phenol resins such as novolac andthe like; epoxy resin; polyurethane; polystyrene, butadiene-styrenecopolymer; polysaccharides such as cellulose, acetyl cellulose,nitrocellulose, starch, calcium alginate, hydroxypropyl methyl celluloseand the like; chitin; chitosan; urushi (Japanese lacquer); polyamidessuch as gelatin, collagen, keratin and the like; and copolymers of thesehigh molecular materials. These may be a composite compound, and metaloxide particles, glass fiber or the like may be added thereto asoccasion demands. Further, an organic compound material may be blendedtherewith.

[0106] Since the capability of attenuating radiation energy generallyincreases as specific gravity increases, in the case where the substrateor the perforated plate of the biochemical analysis unit is made of amaterial capable of attenuating radiation energy in accordance with thepresent invention, the substrate or the perforated plate of thebiochemical analysis unit is preferably formed of a compound material ora composite material having specific gravity of 1.0 g/cm³ or more andmore preferably formed of a compound material or a composite materialhaving specific gravity of 1.5 g/cm³ to 23 g/cm³.

[0107] In the present invention, illustrative examples of inorganiccompound material capable of attenuating light energy and preferablyusable for forming the substrate or the perforated plate of thebiochemical analysis unit in the present invention include metals suchas gold, silver, copper, zinc, aluminum, titanium, tantalum, chromium,iron, nickel, cobalt, lead, tin, selenium and the like; alloys such asbrass, stainless steel, bronze and the like; silicon materials such assilicon, amorphous silicon, glass, quartz, silicon carbide, siliconnitride and the like; metal oxides such as aluminum oxide, magnesiumoxide, zirconium oxide and the like; and inorganic salts such astungsten carbide, calcium carbide, calcium sulfate, hydroxy apatite,gallium arsenide and the like. These may have either a monocrystalstructure or a polycrystal sintered structure such as amorphous, ceramicor the like.

[0108] In the present invention, a high molecular compound is preferablyused as an organic compound material capable of attenuating lightenergy. Illustrative examples of high molecular compounds preferablyusable for forming a substrate or a perforated plate of a biochemicalanalysis unit in the present invention include polyolefins such aspolyethylene, polypropylene and the like; acrylic resins such aspolymethyl methacrylate, polybutylacrylate/polymethyl methacrylatecopolymer and the like; polyacrylonitrile; polyvinyl chloride;polyvinylidene chloride; polyvinylidene fluoride;polytetrafluoroethylene; polychlorotrifuluoroethylene; polycarbonate;polyesters such as polyethylene naphthalate, polyethylene terephthalateand the like; nylons such as nylon-6, nylon-6, 6, nylon-4, 10 and thelike; polyimide; polysulfone; polyphenylene sulfide; silicon resins suchas polydiphenyl siloxane and the like; phenol resins such as novolac andthe like; epoxy resin; polyurethane; polystyrene, butadiene-styrenecopolymer; polysaccharides such as cellulose, acetyl cellulose,nitrocellulose, starch, calcium alginate, hydroxypropyl methyl celluloseand the like; chitin; chitosan; urushi (Japanese lacquer); polyamidessuch as gelatin, collagen, keratin and the like; and copolymers of thesehigh molecular materials. These may be a composite compound, and metaloxide particles, glass fiber or the like may be added thereto asoccasion demands. Further, an organic compound material may be blendedtherewith.

[0109] Since the capability of attenuating light energy generallyincreases as scattering and/or absorption of light increases, in thecase where the substrate or the perforated plate of the biochemicalanalysis unit is made of a material capable of attenuating light energy,in the present invention, the substrate or the perforated plate of thebiochemical analysis unit preferably has absorbance of 0.3 per cm(thickness) or more and more preferably has absorbance of 1 per cm(thickness) or more. The absorbance can be determined by placing anintegrating sphere immediately behind a plate-like member having athickness of T cm, measuring an amount A of transmitted light at awavelength of probe light or emission light used for measurement by aspectrophotometer, and calculating A/T.

[0110] In the present invention, a light scattering substance or a lightabsorbing substance may be added to the substrate or the perforatedplate of the biochemical analysis unit in order to improve thecapability of attenuating light energy. Particles of a materialdifferent from a material forming the substrate or the perforated plateof the biochemical analysis unit may be preferably used as a lightscattering substance and a pigment or dye may be preferably used as alight absorbing substance.

[0111] In a preferred aspect of the present invention, the substrate ofthe biochemical analysis unit is formed of a flexible material.

[0112] According to this preferred aspect of the present invention,since the substrate of the biochemical analysis unit is formed of aflexible material, the biochemical analysis unit can be bent and bebrought into contact with a hybridization solution, thereby hybridizingspecific binding substances with a substance derived from a livingorganism. Therefore, specific binding substances and a substance derivedfrom a living organism can be hybridized with each other in a desiredmanner using a small amount of a hybridization solution.

[0113] In a preferred aspect of the present invention, the plurality ofholes are regularly formed in the substrate of the biochemical analysisunit.

[0114] In a preferred aspect of the present invention, a plurality ofholes having a substantially circular shape are formed in the substrateof the biochemical analysis unit.

[0115] In another preferred aspect of the present invention, a pluralityof holes having a substantially rectangular shape are formed in thesubstrate of the biochemical analysis unit.

[0116] In a preferred aspect of the present invention, the substrate ofthe biochemical analysis unit is formed with 10 or more holes.

[0117] In a further preferred aspect of the present invention, thesubstrate of the biochemical analysis unit is formed with 50 or moreholes.

[0118] In a further preferred aspect of the present invention, thesubstrate of the biochemical analysis unit is formed with 100 or moreholes.

[0119] In a further preferred aspect of the present invention, thesubstrate of the biochemical analysis unit is formed with 1,000 or moreholes.

[0120] In a further preferred aspect of the present invention, thesubstrate of the biochemical analysis unit is formed with 10,000 or moreholes.

[0121] In a further preferred aspect of the present invention, thesubstrate of the biochemical analysis unit is formed with 100,000 ormore holes.

[0122] In a preferred aspect of the present invention, each of theplurality of holes formed in the substrate of the biochemical analysisunit has a size of less than 5 mm².

[0123] In a further preferred aspect of the present invention, each ofthe plurality of holes formed in the substrate of the biochemicalanalysis unit has a size of less than 1 mm².

[0124] In a further preferred aspect of the present invention, each ofthe plurality of holes formed in the substrate of the biochemicalanalysis unit has a size of less than 0.5 mm².

[0125] In a further preferred aspect of the present invention, each ofthe plurality of holes formed in the substrate of the biochemicalanalysis unit has a size of less than 0.1 mm².

[0126] In a further preferred aspect of the present invention, each ofthe plurality of holes formed in the substrate of the biochemicalanalysis unit has a size of less than 0.05 mm².

[0127] In a further preferred aspect of the present invention, each ofthe plurality of holes formed in the substrate of the biochemicalanalysis unit has a size of less than 0.01 mm².

[0128] In the present invention, the density of the holes formed in thesubstrate of the biochemical analysis unit is determined depending uponthe material of the substrate, the thickness of the substrate, the kindof electron beam released from a radioactive substance, the wavelengthof fluorescence released from a fluorescent substance or the like.

[0129] In a preferred aspect of the present invention, the plurality ofholes are formed in the substrate of the biochemical analysis unit at adensity of 10 or more per cm².

[0130] In a further preferred aspect of the present invention, theplurality of holes are formed in the substrate of the biochemicalanalysis unit at a density of 50 or more per cm².

[0131] In a further preferred aspect of the present invention, theplurality of holes are formed in the substrate of the biochemicalanalysis unit at a density of 100 or more per cm².

[0132] In a further preferred aspect of the present invention, theplurality of holes are formed in the substrate of the biochemicalanalysis unit at a density of 500 or more per cm².

[0133] In a further preferred aspect of the present invention, theplurality of holes are formed in the substrate of the biochemicalanalysis unit at a density of 1,000 or more per cm².

[0134] In a further preferred aspect of the present invention, theplurality of holes are formed in the substrate of the biochemicalanalysis unit at a density of 5,000 or more per cm².

[0135] In a further preferred aspect of the present invention, theplurality of holes are formed in the substrate of the biochemicalanalysis unit at a density of 10,000 or more per cm².

[0136] In a preferred aspect of the present invention, a plurality ofthrough-holes are regularly formed in the perforated plate of thebiochemical analysis unit.

[0137] In a preferred aspect of the present invention, a plurality ofthrough-holes having a substantially circular shape are formed in theperforated plate of the biochemical analysis unit.

[0138] In another preferred aspect of the present invention, a pluralityof through-holes having a substantially rectangular shape are formed inthe perforated plate of the biochemical analysis unit.

[0139] In a preferred aspect of the present invention, the perforatedplate of the biochemical analysis unit is formed with 10 or morethrough-holes.

[0140] In a further preferred aspect of the present invention, theperforated plate of the biochemical analysis unit is formed with 50 ormore through-holes.

[0141] In a further preferred aspect of the present invention, theperforated plate of the biochemical analysis unit is formed with 100 ormore through-holes.

[0142] In a further preferred aspect of the present invention, theperforated plate of the biochemical analysis unit is formed with 1,000or more through-holes.

[0143] In a further preferred aspect of the present invention, theperforated plate of the biochemical analysis unit is formed with 10,000or more through-holes.

[0144] In a further preferred aspect of the present invention, theperforated plate of the biochemical analysis unit is formed with 100,000or more through-holes.

[0145] In a preferred aspect of the present invention, each of theplurality of through-holes formed in the perforated plate of thebiochemical analysis unit has a size of less than 5 mm².

[0146] In a further preferred aspect of the present invention, each ofthe plurality of through-holes formed in the perforated plate of thebiochemical analysis unit has a size of less than 1 mm².

[0147] In a further preferred aspect of the present invention, each ofthe plurality of through-holes formed in the perforated plate of thebiochemical analysis unit has a size of less than 0.5 mm².

[0148] In a further preferred aspect of the present invention, each ofthe plurality of through-holes formed in the perforated plate of thebiochemical analysis unit has a size of less than 0.1 mm².

[0149] In a further preferred aspect of the present invention, each ofthe plurality of through-holes formed in the perforated plate of thebiochemical analysis unit has a size of less than 0.05 mm².

[0150] In a further preferred aspect of the present invention, each ofthe plurality of through-holes formed in the perforated plate of thebiochemical analysis unit has a size of less than 0.01 mm².

[0151] In the present invention, the density of the through-holes formedin the perforated plate of the biochemical analysis unit can bearbitrarily determined depending upon the material of the perforatedplate, the thickness of the perforated plate and the kind of electronbeam released from the radioactive labeling substance or the wavelengthof fluorescence released from the fluorescent substance and the like.

[0152] In a preferred aspect of the present invention, the plurality ofthrough-holes are formed in the perforated plate of the biochemicalanalysis unit at a density of 10 or more per cm².

[0153] In a further preferred aspect of the present invention, theplurality of through-holes are formed in the perforated plate of thebiochemical analysis unit at a density of 50 or more per cm².

[0154] In a further preferred aspect of the present invention, theplurality of through-holes are formed in the perforated plate of thebiochemical analysis unit at a density of 100 or more per cm².

[0155] In a further preferred aspect of the present invention, theplurality of through-holes are formed in the perforated plate of thebiochemical analysis unit at a density of 500 or more per cm².

[0156] In a further preferred aspect of the present invention, theplurality of through-holes are formed in the perforated plate of thebiochemical analysis unit at a density of 1,000 or more per cm².

[0157] In a further preferred aspect of the present invention, theplurality of through-holes are formed in the perforated plate of thebiochemical analysis unit at a density of 5,000 or more per cm².

[0158] In a further preferred aspect of the present invention, theplurality of through-holes are formed in the perforated plate of thebiochemical analysis unit at a density of 10,000 or more per cm².

[0159] In the present invention, a porous material or a fiber materialmay be preferably used as the absorptive material for forming theabsorptive region. The absorptive region may be formed by combining aporous material and a fiber material.

[0160] In the present invention, a porous material for forming theabsorptive region may be any type of an organic material or an inorganicmaterial and may be an organic/inorganic composite material.

[0161] In the present invention, an organic porous material used forforming the absorptive region is not particularly limited but a carbonporous material such as an activated carbon or a porous material capableof forming a membrane filter is preferably used. Illustrative examplesof porous materials capable of forming a membrane filter include nylonssuch as nylon-6, nylon-6, 6, nylon-4, 10; cellulose derivatives such asnitrocellulose, acetyl cellulose, butyric-acetyl cellulose; collagen;alginic acids such as alginic acid, calcium alginate, alginicacid/poly-L-lysine polyionic complex; polyolefins such as polyethylene,polypropylene; polyvinyl chloride; polyvinylidene chloride; polyfluoridesuch as polyvinylidene fluoride, polytetrafluoride; and copolymers orcomposite materials thereof.

[0162] In the present invention, an inorganic porous material used forforming the absorptive region is not particularly limited. Illustrativeexamples of inorganic porous materials preferably usable in the presentinvention include metals such as platinum, gold, iron, silver, nickel,aluminum and the like; metal oxides such as alumina, silica, titania,zeolite and the like; metal salts such as hydroxy apatite, calciumsulfate and the like; and composite materials thereof.

[0163] In the present invention, a fiber material used for forming theabsorptive region is not particularly limited. Illustrative examples offiber materials preferably usable in the present invention includenylons such as nylon-6, nylon-6, 6, nylon-4, 10; and cellulosederivatives such as nitrocellulose, acetyl cellulose, butyric-acetylcellulose.

[0164] In the present invention, in the case where a plurality ofdot-like stimulable phosphor layer regions are formed in the support ofthe stimulable phosphor sheet, the plurality of dot-like stimulablephosphor layer regions may be formed on the surface of the support orthe plurality of dot-like stimulable phosphor layer regions may beformed in a plurality of holes formed dot-like in the support.

[0165] In the present invention, in the case where a plurality ofdot-like stimulable phosphor layer regions are formed in the support ofthe stimulable phosphor sheet, the plurality of dot-like stimulablephosphor layer regions are formed in the same pattern as that of theabsorptive regions formed in the biochemical analysis unit.

[0166] In a preferred aspect of the present invention, a plurality ofthrough-holes are formed dot-like in the support of the stimulablephosphor sheet and stimulable phosphor layer regions are formed in theplurality of through-holes.

[0167] In a further preferred aspect of the present invention,stimulable phosphor layer regions are formed by charging stimulablephosphor in the plurality of through-holes.

[0168] In another preferred aspect of the present invention, a pluralityof recesses are dot-like formed in the support of the stimulablephosphor sheet and stimulable phosphor layer regions are formed in theplurality of recesses.

[0169] In a further preferred aspect of the present invention,stimulable phosphor layer regions are formed by charging stimulablephosphor in the plurality of recesses.

[0170] In a preferred aspect of the present invention, a plurality ofdot-like stimulable phosphor layer regions are regularly formed in thestimulable phosphor sheet.

[0171] In the present invention, in the case where a plurality ofdot-like stimulable phosphor layer regions are formed in the support ofthe stimulable phosphor sheet, the material for forming the support ofthe stimulable phosphor sheet preferably has a property of attenuatingradiation energy. The material capable of attenuating radiation energyand usable for forming the support of the stimulable phosphor sheet isnot particularly limited but may be of any type of inorganic compoundmaterial or organic compound material insofar as it can attenuateradiation energy. It is preferably formed of metal material, ceramicmaterial or plastic material.

[0172] In the present invention, illustrative examples of inorganiccompound materials capable of attenuating radiation energy andpreferably usable for forming the support of the stimulable phosphorsheet in the present invention include metals such as gold, silver,copper, zinc, aluminum, titanium, tantalum, chromium, iron, nickel,cobalt, lead, tin, selenium and the like; alloys such as brass,stainless steel, bronze and the like; silicon materials such as silicon,amorphous silicon, glass, quartz, silicon carbide, silicon nitride andthe like; metal oxides such as aluminum oxide, magnesium oxide,zirconium oxide and the like; and inorganic salts such as tungstencarbide, calcium carbide, calcium sulfate, hydroxy apatite, galliumarsenide and the like. These may have either a monocrystal structure ora polycrystal sintered structure such as amorphous, ceramic or the like.

[0173] In the present invention, a high molecular compound is preferablyused as an organic compound material capable of attenuating radiationenergy. Illustrative examples of high molecular compounds and preferablyusable for forming a support of the stimulable phosphor sheet in thepresent invention include polyolefins such as polyethylene,polypropylene and the like; acrylic resins such as polymethylmethacrylate, polybutylacrylate/polymethyl methacrylate copolymer andthe like; polyacrylonitrile; polyvinyl chloride; polyvinylidenechloride; polyvinylidene fluoride; polytetrafluoroethylene;polychlorotrifuluoroethylene; polycarbonate; polyesters such aspolyethylene naphthalate, polyethylene terephthalate and the like;nylons such as nylon-6, nylon-6, 6, nylon-4, 10 and the like; polyimide;polysulfone; polyphenylene sulfide; silicon resins such as polydiphenylsiloxane and the like; phenol resins such as novolac and the like; epoxyresin; polyurethane; polystyrene, butadiene-styrene copolymer;polysaccharides such as cellulose, acetyl cellulose, nitrocellulose,starch, calcium alginate, hydroxypropyl methyl cellulose and the like;chitin; chitosan; urushi (Japanese lacquer); polyamides such as gelatin,collagen, keratin and the like; and copolymers of these high molecularmaterials. These may be a composite compound, and metal oxide particles,glass fiber or the like may be added thereto as occasion demands.Further, an organic compound material may be blended therewith.

[0174] Since the capability of attenuating radiation energy generallyincreases as specific gravity increases, the support of the stimulablephosphor sheet is preferably formed of a compound material or acomposite material having specific gravity of 1.0 g/cm³ or more and morepreferably formed of a compound material or a composite material havingspecific gravity of 1.5 g/cm³ to 23 g/cm³.

[0175] In a preferred aspect of the present invention, a materialcapable of attenuating radiation energy has property of reducing theenergy of radiation to ⅕ or less when the radiation travels in thematerial by the distance between neighboring dot-like stimulablephosphor layer regions.

[0176] In a further preferred aspect of the present invention, thesupport of the stimulable phosphor sheet is made of a material capableof reducing the energy of radiation to {fraction (1/10)} or less whenthe radiation travels in the material by the distance betweenneighboring dot-like stimulable phosphor layer regions.

[0177] In a further preferred aspect of the present invention, thesupport of the stimulable phosphor sheet is made of a material capableof reducing the energy of radiation to {fraction (1/50)} or less whenthe radiation travels in the material by the distance betweenneighboring dot-like stimulable phosphor layer regions.

[0178] In a further preferred aspect of the present invention, thesupport of the stimulable phosphor sheet is made of a material capableof reducing the energy of radiation to {fraction (1/100)} or less whenthe radiation travels in the material by the distance betweenneighboring dot-like stimulable phosphor layer regions.

[0179] In a further preferred aspect of the present invention, thesupport of the stimulable phosphor sheet is made of a material capableof reducing the energy of radiation to {fraction (1/500)} or less whenthe radiation travels in the material by the distance betweenneighboring dot-like stimulable phosphor layer regions.

[0180] In a further preferred aspect of the present invention, thesupport of the stimulable phosphor sheet is made of a material capableof reducing the energy of radiation to {fraction (1/1000)} or less whenthe radiation travels in the material by the distance betweenneighboring dot-like stimulable phosphor layer regions.

[0181] The above and other objects of the present invention can also beaccomplished by a biochemical analyzing method comprising the steps ofpreparing a stimulable phosphor sheet including a support, selectivelystoring radiation energy in a plurality of stimulable phosphor layerregions formed at least one-dimensionally and spaced-apart from eachother in the support, moving the stimulable phosphor sheet and astimulating ray relative to each other in at least a main scanningdirection, sequentially irradiating the plurality of stimulable phosphorlayer regions with the stimulating ray, thereby exciting stimulablephosphor contained in the plurality of stimulable phosphor layerregions, photoelectrically detecting stimulated emission released fromthe stimulable phosphor, thereby producing analog data, converting theanalog data to digital data and producing biochemical analysis data.

[0182] According to this aspect of the present invention, sincebiochemical analysis data are produced by selectively storing radiationenergy in a plurality of stimulable phosphor layer regions formed atleast one-dimensionally and spaced-apart from each other in the support,moving the stimulable phosphor sheet and a stimulating ray relative toeach other in at least a main scanning direction, sequentiallyirradiating the plurality of stimulable phosphor layer regions with thestimulating ray, thereby exciting stimulable phosphor contained in theplurality of stimulable phosphor layer regions, photoelectricallydetecting stimulated emission released from the stimulable phosphor,thereby producing analog data, and converting the analog data to digitaldata, it is possible to produce biochemical analysis data with highresolving power and high quantitative accuracy.

[0183] In a preferred aspect of the present invention, the plurality ofstimulable phosphor layer regions are formed two-dimensionally andspaced-apart from each other in the support and biochemical analysisdata are produced by moving the stimulable phosphor sheet and thestimulating ray relative to each other in the main scanning directionand a sub-scanning direction perpendicular to the main scanningdirection, sequentially irradiating the plurality of stimulable phosphorlayer regions with the stimulating ray, thereby exciting stimulablephosphor contained in the plurality of stimulable phosphor layerregions, photoelectrically detecting stimulated emission released fromthe stimulable phosphor, thereby producing analog data, and convertingthe analog data to digital data.

[0184] According to this preferred aspect of the present invention,since the stimulable phosphor layer regions can be formed at a highdensity, biochemical analysis data can be efficiently produced.

[0185] In a preferred aspect of the present invention, a laser beam isused as a stimulating ray and stimulable phosphor contained in theplurality of stimulable phosphor layer regions is excited by moving thestimulable phosphor sheet and the laser beam relative to each other inthe main scanning direction and a sub-scanning direction perpendicularto the main scanning direction, and sequentially irradiating theplurality of stimulable phosphor layer regions with the laser beam.

[0186] In a preferred aspect of the present invention, the stimulablephosphor sheet is moved in the main scanning direction.

[0187] In another preferred aspect of the present invention, thestimulating ray is moved in the main scanning direction.

[0188] In the present invention, the stimulable phosphor usable in thepresent invention may be of any type insofar as it can store radiationenergy or electron beam energy and can be stimulated by anelectromagnetic wave to release the radiation energy or the electronbeam energy stored therein in the form of light. More specifically,preferably employed stimulable phosphors include alkaline earth metalfluorohalide phosphors (Ba_(1−x), M²⁺ _(x))FX:yA (where M²⁺ is at leastone alkaline earth metal selected from the group consisting of Mg, Ca,Sr, Zn and Cd; X is at least one element selected from the groupconsisting of Cl, Br and I, A is at least one element selected from thegroup consisting of Eu, Tb, Ce, Tm, Dy, Pr, Ho, Nd, Yb and Er; x isequal to or greater than 0 and equal to or less than 0.6 and y is equalto or greater than 0 and equal to or less than 0.2) disclosed in U.S.Pat. No. 4,239,968, alkaline earth metal fluorohalide phosphors SrFX:Z(where X is at least one halogen selected from the group consisting ofCl, Br and I; Z is at least one Eu and Ce) disclosed in Japanese PatentApplication Laid Open No. 2-276997, europium activated complex halidephosphors BaFXxNaX′:aEu²⁺ (where each of X or X′ is at least one halogenselected from the group consisting of Cl, Br and I; x is greater than 0and equal to or less than 2; and y is greater than 0 and equal to orless than 0.2) disclosed in Japanese Patent Application Laid Open No.59-56479, cerium activated trivalent metal oxyhalide phosphors MOX:xCe(where M is at least one trivalent metal selected from the groupconsisting of Pr, Nd, Pm, Sm, Eu, Tb, Dy, Ho, Er, Tm, Yb and Bi; X is atleast one halogen selected from the group consisting of Br and I; and xis greater than 0 and less than 0.1) disclosed in Japanese PatentApplication laid Open No. 58-69281, cerium activated rare earthoxyhalide phosphors LnOX:xCe (where Ln is at least one rare earthelement selected from the group consisting of Y, La, Gd and Lu; X is atleast one halogen selected from the group consisting of Cl, Br and I;and x is greater than 0 and equal to or less than 0.1) disclosed in U.S.Pat. No. 4,539,137, and europium activated complex halide phosphorsM^(II)FXaM^(I)X′bM′^(II)X″₂cM^(III)X′″₃xA:yEu²⁺ (where M^(II) is atleast one alkaline earth metal selected from the group consisting of Ba,Sr and Ca; M^(I) is at least one alkaline metal selected from the groupconsisting of Li, Na, K, Rb and Cs; M′^(II) is at least one divalentmetal selected from the group consisting of Be and Mg; M^(III) is atleast one trivalent metal selected from the group consisting of Al, Ga,In and Ti; Ais at least one metal oxide; X is at least one halogenselected from the group consisting of Cl, Br and I; each of X′, X″ andX′″ is at least one halogen selected from the group consisting of F, Cl,Br and I; a is equal to or greater than 0 and equal to or less than 2; bis equal to or greater than 0 and equal to or less than 10⁻²; c is equalto or greater than 0 and equal to or less than 10⁻²; a+b+c is equal toor greater than 10⁻²; x is greater than 0 and equal to or less than 0.5;and y is greater than 0 and equal to or less than 0.2) disclosed in U.S.Pat. No. 4,962,047.

[0189] In a preferred aspect of the present invention, specific bindingsubstances may be spotted onto the absorptive regions of a biochemicalanalysis unit using a spotting device.

[0190] In a preferred aspect of the present invention, a spotting deviceincludes a base plate onto which a biochemical analysis unit, on whichspecific binding substances are to be spotted, is to be placed, aspotting head capable of spotting specific binding substances, andsensor means for detecting a reference position of the absorptive regionto which specific binding substances are to be spotted.

[0191] In a preferred aspect of the present invention, a spotting devicefurther includes a drive mechanism for at least one-dimensionally andintermittently moving the spotting head and the base plate relative toeach other.

[0192] According to this preferred aspect of the present invention,since a spotting device further includes a drive mechanism for at leastone-dimensionally and intermittently moving the spotting head and thebase plate relative to each other, specific binding substances can bereliably spotted onto the absorptive regions formed in a biochemicalanalysis unit in at least one-dimension by using the sensor means todetect the absorptive regions of the biochemical analysis unit placed onthe base plate for spotting with specific binding substances, therebydetermining the relative positional relationship between the spottinghead of the spotting device and the base plate on which the biochemicalanalysis unit is placed, and spotting specific binding substances fromthe spotting head, while operating the driving mechanism for at leastone dimensionally and intermittently moving the spotting head and thebase plate relative to each other.

[0193] In a further preferred aspect of the present invention, the drivemechanism is adapted for at least one-dimensionally moving the spottinghead and the base plate relative to each other at a constant pitch.

[0194] According to this preferred aspect of the present invention,since the drive mechanism is adapted for at least one-dimensionallymoving the spotting head and the base plate relative to each other at aconstant pitch, specific binding substances can be reliably spotted ontothe absorptive regions formed in a biochemical analysis unit in at leastone-dimension by using the sensor means to detect the absorptive regionsof the biochemical analysis unit placed on the base plate for spottingwith specific binding substances, thereby determining the relativepositional relationship between the spotting head of the spotting deviceand the base plate on which the biochemical analysis unit is placed, andspotting specific binding substances from the spotting head, whileoperating the driving mechanism for at least one dimensionally movingthe spotting head and the base plate relative to each other at aconstant pitch.

[0195] In a further preferred aspect of the present invention, the drivemechanism is adapted for relatively and intermittently moving thespotting head and the base in two dimensions.

[0196] According to this preferred aspect of the present invention,since the drive mechanism is adapted for relatively and intermittentlymoving the spotting head and the base in two dimensions, specificbinding substances can be reliably spotted onto the absorptive regionstwo-dimensionally formed in a biochemical analysis unit by using thesensor means to detect the absorptive regions of the biochemicalanalysis unit placed on the base plate for spotting with specificbinding substances, thereby determining the relative positionalrelationship between the spotting head of the spotting device and thebase plate on which the biochemical analysis unit is placed, andspotting specific binding substances from the spotting head, whileoperating the driving mechanism for relatively and intermittently movingthe spotting head and the base plate in two dimensions.

[0197] In a further preferred aspect of the present invention, the drivemechanism is adapted for relatively and intermittently moving thespotting head and the base at a constant pitch in two dimensions.

[0198] According to this preferred aspect of the present invention,since the drive mechanism is adapted for relatively and intermittentlymoving the spotting head and the base at a constant pitch in twodimensions, specific binding substances can be reliably spotted onto theabsorptive regions two-dimensionally formed in a biochemical analysisunit by using the sensor means to detect the absorptive regions of thebiochemical analysis unit placed on the base plate for spotting withspecific binding substances, thereby determining the relative positionalrelationship between the spotting head of the spotting device and thebase plate on which the biochemical analysis unit is placed, andspotting specific binding substances from the spotting head, whileoperating the driving mechanism for relatively and intermittently movingthe spotting head and the base plate at a constant pitch in twodimensions.

[0199] In a preferred aspect of the present invention, at least twopositioning members are formed in the base plate for positioning abiochemical analysis unit.

[0200] According to this preferred aspect of the present invention,since at least two positioning members are formed in the base plate forpositioning a biochemical analysis unit, it is possible to position thebiochemical analysis unit onto which specific binding substances are tobe spotted at a predetermined position of the base plate and set it onthe base plate.

[0201] In a further preferred aspect of the present invention, each ofthe positioning members is constituted as a pin uprightly formed on thebase plate.

[0202] According to this preferred aspect of the present invention,since each of the positioning members is constituted as a pin uprightlyformed on the base plate, it is possible to easily position thebiochemical analysis unit onto which specific binding substances are tobe spotted at a predetermined position of the base plate and set it onthe base plate by forming the biochemical analysis unit with positioningthrough-holes corresponding to the pins.

[0203] In a preferred aspect of the present invention, the spottingdevice further includes positional data calculating means forcalculating positional data of the absorptive regions of the biochemicalanalysis unit onto which specific binding substances are to be spottedbased on at least two reference positions of the biochemical analysisunit detected by the sensor means, a memory for storing the positionaldata of the absorptive regions of the biochemical analysis unit ontowhich specific binding substances are to be spotted calculated by thepositional data calculating means, and position control means forcontrolling the drive mechanism in accordance with the positional dataof the absorptive regions of the biochemical analysis unit onto whichspecific binding substances are to be spotted stored in the memory.

[0204] According to this preferred aspect of the present invention,since the spotting device further includes positional data calculatingmeans for calculating positional data of the absorptive regions of thebiochemical analysis unit onto which specific binding substances are tobe spotted based on at least two references positions of the biochemicalanalysis unit detected by the sensor means, a memory for storing thepositional data of the absorptive regions of the biochemical analysisunit onto which specific binding substances are to be spotted calculatedby the positional data calculating means, and position control means forcontrolling the drive mechanism in accordance with the positional dataof the absorptive regions of the biochemical analysis unit onto whichspecific binding substances are to be spotted stored in the memory, itis possible to automatically spot specific binding substances onto aplurality of absorptive regions spaced-apart and dot-like formed in thesubstrate.

[0205] The above and other objects and features of the present inventionwill become apparent from the following description made with referenceto the accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

[0206]FIG. 1 is a schematic perspective view showing a biochemicalanalysis unit which is a preferred embodiment of the present invention.

[0207]FIG. 2 is a schematic front view showing a spotting device.

[0208]FIG. 3 is a schematic front view showing a hybridization vessel.

[0209]FIG. 4 is a schematic perspective view showing a stimulablephosphor sheet.

[0210]FIG. 5 is a schematic cross-sectional view showing a method forexposing a number of dot-like stimulable phosphor layer regions formedon a stimulable phosphor sheet by a radioactive labeling substancecontained in absorptive regions formed in a number of through-holes.

[0211]FIG. 6 is a schematic perspective view showing one example of ascanner for reading radiation data of a radioactive labeling substancerecorded in a number of stimulable phosphor layer regions formed on astimulable phosphor sheet and fluorescence data recorded in absorptiveregions formed in a number of holes of a biochemical analysis unit andproducing biochemical analysis data.

[0212]FIG. 7 is a schematic perspective view showing details in thevicinity of a photomultiplier.

[0213]FIG. 8 is a schematic cross-sectional view taken along a line A-Ain FIG. 7.

[0214]FIG. 9 is a schematic cross-sectional view taken along a line B-Bin FIG. 7.

[0215]FIG. 10 is a schematic cross-sectional view taken along a line C-Cin FIG. 7.

[0216]FIG. 11 is a schematic cross-sectional view taken along a line D-Din FIG. 7.

[0217]FIG. 12 is a schematic plan view of a scanning mechanism of anoptical head.

[0218]FIG. 13 is a block diagram of a control system, an input systemand a drive system of a scanner shown in FIG. 6.

[0219]FIG. 14 is a schematic front view showing a data producing systemfor reading chemiluminescent data of a labeling substance, recorded inabsorptive regions formed in a number of through-holes of a biochemicalanalysis unit, which generates chemiluminescent emission when itcontacts a chemiluminescent substrate and producing a biochemicalanalysis data.

[0220]FIG. 15 is a schematic longitudinal cross sectional view showing acooled CCD camera.

[0221]FIG. 16 is a schematic vertical cross sectional view showing adark box.

[0222]FIG. 17 is a block diagram of a personal computer and peripheraldevices thereof.

[0223]FIG. 18 is a schematic vertical cross sectional view showinganother example of a dark box.

[0224]FIG. 19 is a schematic longitudinal cross sectional view showing abiochemical analysis unit which is another embodiment of the presentinvention.

[0225]FIG. 20 is a schematic cross-sectional view showing a method forexposing a number of dot-like stimulable phosphor layer regions formedon a stimulable phosphor sheet by a radioactive labeling substancecontained in absorptive regions formed on a porous substrate.

[0226]FIG. 21 is a schematic perspective view of a biochemical analysisunit which is a further preferred embodiment of the present invention.

[0227]FIG. 22 is a schematic plan view showing another example of aspotting device.

[0228]FIG. 23 is a block diagram showing a control system, an inputsystem, a drive system and a detection system of a spotting device.

[0229]FIG. 24 is a schematic partial plan view showing a biochemicalanalysis unit in which specific binding substances are spotted from aninjector located a reference position thereof.

[0230]FIG. 25 is a schematic perspective view of a biochemical analysisunit which is a further preferred embodiment of the present invention.

DESCRIPTION OF THE PREFERRED EMBODIMENT

[0231]FIG. 1 is a schematic perspective view showing a biochemicalanalysis unit which is a preferred embodiment of the present invention.

[0232] As shown in FIG. 1, a biochemical analysis unit 1 includes asubstrate 2 formed of metal such as aluminum capable of attenuatingradiation energy and light energy and having flexibility and formed witha number of substantially circular through-holes 3, and absorptivematerial 4 such as nylon-6 is charged in the through-holes 3.

[0233] Although not accurately shown in FIG. 1, in this embodiment,about 10,000 through-holes 3 having a size of about 0.01 cm² areregularly formed at a density of about 10,000 per cm² in the substrate2.

[0234]FIG. 2 is a schematic front view showing a spotting device.

[0235] When biochemical analysis is performed, as shown in FIG. 2,specific binding substances such as a plurality of cDNAs whose sequencesare known but are different from each other are spotted using a spottingdevice onto the porous material 4 charged in a number of thethrough-holes 3 of the biochemical analysis unit 1.

[0236] As shown in FIG. 2, the spotting head 5 of the spotting deviceincludes an injector 6 for ejecting a solution of specific bindingsubstances toward the biochemical analysis unit 1 and a CCD camera 7 andis constituted so that cDNAs are spotted from the injector 6 when thetip end portion of the injector 6 and the center of the through-hole 3into which a specific binding substance is to be spotted are determinedto coincide with each other as a result of viewing them using the CCDcamera, thereby ensuring that cDNAs can be accurately spotted into thethrough-hole 3 in which porous material is charged.

[0237]FIG. 3 is a schematic front view showing a hybridization vessel.

[0238] As shown in FIG. 3, a hybridization vessel 8 is formedcylindrically and accommodates a hybridization solution 9 containing asubstance derived from a living organism labeled with a labelingsubstance therein.

[0239] In the case where a specific binding substance such as cDNA is tobe labeled with a radioactive labeling substance, a hybridizationsolution 9 containing a substance derived from a living organism labeledwith a radioactive labeling substance is prepared and is accommodated inthe hybridization vessel 8.

[0240] On the other hand, in the case where a specific binding substancesuch as cDNA is to be labeled with a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrate,a hybridization solution 9 containing a substance derived from a livingorganism labeled with a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateis prepared and is accommodated in the hybridization vessel 8.

[0241] Further, in the case where a specific binding substance such ascDNA is to be labeled with a fluorescent substance such as a fluorescentdye, a hybridization solution 9 containing a substance derived from aliving organism labeled with a fluorescent substance such as afluorescent dye is prepared and is accommodated in the hybridizationvessel 8.

[0242] It is possible to prepare a hybridization solution 9 containingtwo or more substances derived from a living organism among a substancederived from a living organism labeled with a radioactive labelingsubstance, a substance derived from a living organism labeled with alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate and a substance derived from aliving organism labeled with a fluorescent substance such as afluorescent dye and accommodate it in the hybridization vessel 8. Inthis embodiment, a hybridization solution 9 containing a substancederived from a living organism labeled with a radioactive labelingsubstance, a substance derived from a living organism labeled with alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate and a substance derived from aliving organism labeled with a fluorescent substance such as afluorescent dye is prepared and accommodated in the hybridization vessel8.

[0243] When hybridization is to be performed, the biochemical analysisunit 1 containing specific binding substances such as a plurality ofcDNAs spotted into a number of through-holes 3 in which porous materialis charged is accommodated in the hybridization vessel 8. In thisembodiment, since the substrate 2 is formed of a metal havingflexibility, as shown in FIG. 3, the biochemical analysis unit 1 can bebent and accommodated in the hybridization vessel 8 along the inner wallsurface thereof.

[0244] As shown in FIG. 3, the hybridization vessel 8 is constituted soas to be rotatable about a shaft by a drive means (not shown) and sincethe biochemical analysis unit 1 is bent and accommodated in thehybridization vessel 8 along the inner wall surface thereof, even whenthe hybridization vessel 8 accommodates only a small amount ofhybridization solution 9, specific binding substances spotted in anumber of the through-holes 3 charged with porous material can beselectively hybridized with a substance derived from a living organismlabeled with a radioactive labeling substance, a substance derived froma living organism labeled with a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand a substance derived from a living organism labeled with afluorescent substance such as a fluorescent dye by rotating thehybridization vessel 8.

[0245] As a result of the hybridization, fluorescence data of afluorescent substance such as a fluorescent dye and chemiluminescencedata of a substance derived from a living organism labeled with alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate are recorded in the porousmaterial 4 charged in a number of the through-holes 3 of the biochemicalanalysis unit 1. Fluorescence data recorded in the porous material 4 areread by a scanner described later, thereby producing biochemicalanalysis data and chemiluminescence data recorded in the porous material4 are read by a data producing system described later, thereby producingbiochemical analysis data.

[0246]FIG. 4 is a schematic perspective view showing a stimulablephosphor sheet.

[0247] As shown in FIG. 4, a stimulable phosphor sheet 10 includes asupport 11 and one surface of the support 11 is formed with a number ofdot-like substantially circular stimulable phosphor layer regions 12 inthe same regular pattern as that of a number of through-holes 3 formedin the biochemical analysis unit 1.

[0248] In this embodiment, the support 11 is formed of stainless capableof attenuating radiation energy.

[0249]FIG. 5 is a schematic cross-sectional view showing a method forexposing a number of dot-like stimulable phosphor layer regions 12formed on the stimulable phosphor sheet 10 to a radioactive labelingsubstance contained in the absorptive regions 4 formed in a number ofthrough-holes 3.

[0250] As shown in FIG. 5, when the stimulable phosphor sheet 10 is tobe exposed, the stimulable phosphor sheet 10 is superposed on thebiochemical analysis unit 1 in such a manner that each of the dot-likestimulable phosphor layer regions 12 formed in the stimulable phosphorsheet 10 is located in one of the many through-holes 3 formed in thebiochemical analysis unit 1 and that the surface of each of the dot-likestimulable phosphor layer regions 12 comes into close contact with thesurface of the porous material 4 charged in one of the through-holes 3.

[0251] In this embodiment, since the substrate 2 of the biochemicalanalysis unit 1 is formed of a metal, it is hardly stretched and shrunkeven when it is subjected to liquid processing such as hybridizationand, therefore, it is possible to easily and accurately superpose thestimulable phosphor sheet 10 on the biochemical analysis unit 1 so thateach of the dot-like stimulable phosphor layer regions 12 formed in thestimulable phosphor sheet 10 is located in one of the many through-holes3 formed in the biochemical analysis unit 1 and that the surface of eachof the dot-like stimulable phosphor layer regions 12 comes into closecontact with the surface of the porous material 4 charged in one of thethrough-holes 3, thereby exposing the dot-like stimulable phosphor layerregions 12.

[0252] In this manner, the surface of each of the dot-like stimulablephosphor layer regions 12 is kept in close contact with the surface ofthe porous material 4 charged in one of the through-holes 3 for apredetermined time period, whereby a number of the dot-like stimulablephosphor layer regions 12 formed in the stimulable phosphor sheet 10 areexposed to the radioactive labeling substance contained in the porousmaterial 4.

[0253] During the exposure operation, electron beams are released fromthe radioactive labeling substance. However, since the substrate 2 isformed of a metal capable of attenuating radiation energy and lightenergy, electron beams released from the radioactive labeling substanceare prevented from being scattered in the substrate 2. Further, sinceeach of a number of dot-like stimulable phosphor layer regions 12 formedin the stimulable phosphor sheet 10 is located in one of the manythrough-holes 3 formed in the biochemical analysis unit 1, the electronbeams released from the radioactive labeling substance is prevented frombeing scattered in the dot-like stimulable phosphor layer region 12 andadvancing to the dot-like stimulable phosphor layer region 12 located inneighboring through-holes.

[0254] Moreover, since the support 11 of the stimulable phosphor sheet10 is formed of stainless capable of attenuating radiation energy inthis embodiment, the electron beams can be also prevented from beingscattered in the support 11 of the stimulable phosphor sheet 10 to enterneighboring dot-like stimulable phosphor layers region 12.

[0255] Therefore, it is possible to reliably expose a number of thedot-like stimulable phosphor layer regions 12 formed in the stimulablephosphor sheet 10 to only the radioactive labeling substance containedin the porous material 4 charged in the corresponding through-holes 3.

[0256] In this manner, radiation data of a radioactive labelingsubstance are recorded in a number of the dot-like stimulable phosphorlayer regions 12 formed in the stimulable phosphor sheet 10.

[0257]FIG. 6 is a schematic perspective view showing one example of ascanner for reading radiation data of a radioactive labeling substancerecorded in a number of the dot-like stimulable phosphor layer regions12 formed on the stimulable phosphor sheet 10 and fluorescence datarecorded in the absorptive regions 4 formed in a number of through-holes3 of the biochemical analysis unit 1 and producing biochemical analysisdata, and FIG. 7 is a schematic perspective view showing details in thevicinity of a photomultiplier.

[0258] The scanner shown in FIG. 6 is constituted so as to readradiation data of a radioactive labeling substance recorded in a numberof the dot-like stimulable phosphor layer regions 12 formed on thestimulable phosphor sheet 10 and fluorescence data recorded in theporous material 4 charged in a number of through-holes 3 formed in thebiochemical analysis unit 1 and includes a first laser stimulating raysource 21 for emitting a laser beam having a wavelength of 640 nm, asecond laser stimulating ray source 22 for emitting a laser beam havinga wavelength of 532nm and a third laser stimulating ray source 23 foremitting a laser beam having a wavelength of 473 nm. In this embodiment,the first laser stimulating ray source 21 constituted by a semiconductorlaser beam source and the second laser stimulating ray source 22 and thethird laser stimulating ray source 23 are constituted by a secondharmonic generation element.

[0259] A laser beam 24 emitted from the first laser stimulating source21 passes through a collimator lens 25, thereby being made a parallelbeam, and is reflected by a mirror 26. A first dichroic mirror 27 fortransmitting light having a wavelength of 640 nm but reflecting lighthaving a wavelength of 532 nm and a second dichroic mirror 28 fortransmitting light having a wavelength equal to and longer than 532 nmbut reflecting light having a wavelength of 473 nm are provided in theoptical path of the laser beam 24 emitted from the first laserstimulating ray source 21. The laser beam 24 emitted from the firstlaser stimulating ray source 21 and reflected by the mirror 26 passesthrough the first dichroic mirror 27 and the second dichroic mirror 28and advances to a mirror 29.

[0260] On the other hand, the laser beam 24 emitted from the secondlaser stimulating ray source 22 passes through a collimator lens 30,thereby being made a parallel beam, and is reflected by the firstdichroic mirror 27, thereby changing its direction by 90 degrees. Thelaser beam 24 then passes through the second dichroic mirror 28 andadvances to the mirror 29.

[0261] Further, the laser beam 24 emitted from the third laserstimulating ray source 23 passes through a collimator lens 31, therebybeing made a parallel beam, and is reflected by the second dichroicmirror 28, thereby changing its direction by 90 degrees. The laser beam24 then advances to the mirror 29.

[0262] The laser beam 24 advancing to the mirror 29 is reflected by themirror 29 and advances to a mirror 32 to be reflected thereby.

[0263] A perforated mirror 34 formed with a hole 33 at the centerportion thereof is provided in the optical path of the laser beam 24reflected by the mirror 32. The laser beam 4 reflected by the mirror 32passes through the hole 33 of the perforated mirror 34 and advances to aconcave mirror 38.

[0264] The laser beam 24 advancing to the concave mirror 38 is reflectedby the concave mirror 38 and enters an optical head 35.

[0265] The optical head 35 includes a mirror 36 and an aspherical lens37. The laser beam 24 entering the optical head 35 is reflected by themirror 36 and condensed by the aspherical lens 37 onto the stimulablephosphor sheet 10 or the biochemical analysis unit 1 placed on the glassplate 41 of the stage 40. In FIG. 6, the biochemical analysis unit 1 isplaced on the glass plate 41 of the stage 40 in such a manner that theside of the porous material 4 into which a specific binding substance isdropped is directed downward.

[0266] When the laser beam 24 impinges on the dot-like stimulablephosphor layer region 12 of the stimulable phosphor 10, stimulablephosphor contained in the dot-like stimulable phosphor layer region 12formed on the stimulable phosphor 10 is excited, thereby releasingstimulated emission 45. On the other hand, when the laser beam 24impinges on the biochemical analysis unit 1, a fluorescent dye or thelike contained in the porous material 4 in a number of the through-holes3 is excited, thereby releasing fluorescence 45.

[0267] The stimulated emission 45 released from the dot-like stimulablephosphor layer region 12 of the stimulable phosphor 10 or thefluorescence 45 released from the porous material 4 in a number of thethrough-holes 3 of the biochemical analysis unit 1 is condensed onto themirror 36 by the aspherical lens 37 provided in the optical head 35 andreflected by the mirror 36 on the side of the optical path of the laserbeam 24, thereby being made a parallel beam to advance to the concavemirror 38.

[0268] The stimulated emission 45 or the fluorescence 45 advancing tothe concave mirror 38 is reflected by the concave mirror 38 and advancesto the perforated mirror 34.

[0269] As shown in FIG. 7, the stimulated emission 45 or thefluorescence 45 advancing to the perforated mirror 34 is reflecteddownward by the perforated mirror 34 formed as a concave mirror andadvances to a filter unit 48, whereby light having a predeterminedwavelength is cut. The stimulated emission 45 or the fluorescence 45then impinges on a photomultiplier 50, thereby being photoelectricallydetected.

[0270] As shown in FIG. 7, the filter unit 48 is provided with fourfilter members 51 a, 51 b, 51 c and 51 d and is constituted to belaterally movable in FIG. 7 by a motor (not shown).

[0271]FIG. 8 is a schematic cross-sectional view taken along a line A-Ain FIG. 7.

[0272] As shown in FIG. 8, the filter member 51 a includes a filter 52 aand the filter 52 a is used for reading fluorescence 45 by stimulating afluorescent substance such as a fluorescent dye contained in the porousmaterial 4 in a number of through-holes 3 of the biochemical analysisunit 1 using the first laser stimulating ray source 21 and has aproperty of cutting off light having a wavelength of 640 nm buttransmitting light having a wavelength longer than 640 nm.

[0273]FIG. 9 is a schematic cross-sectional view taken along a line B-Bin FIG. 7.

[0274] As shown in FIG. 9, the filter member 51 b includes a filter 52 band the filter 52 b is used for reading fluorescence 45 by stimulating afluorescent substance such as a fluorescent dye contained in the porousmaterial 4 in a number of through-holes 3 of the biochemical analysisunit 1 using the second laser stimulating ray source 22 and has aproperty of cutting off light having a wavelength of 532 nm buttransmitting light having a wavelength longer than 532 nm.

[0275]FIG. 10 is a schematic cross-sectional view taken along a line C-Cin FIG. 7.

[0276] As shown in FIG. 10, the filter member 51 a includes a filter 52c and the filter 52 c is used for reading fluorescence 45 by stimulatinga fluorescent substance such as a fluorescent dye contained in theporous material 4 in a number of through-holes 3 of the biochemicalanalysis unit 1 using the third laser stimulating ray source 23 and hasa property of cutting off light having a wavelength of 473 nm buttransmitting light having a wavelength longer than 473 nm.

[0277]FIG. 11 is a schematic cross-sectional view taken along a line D-Din FIG. 7.

[0278] As shown in FIG. 11, the filter member 51 d includes a filter 52d and the filter 52 d is used for reading stimulated emission releasedfrom stimulable phosphor contained in the dot-like stimulable phosphorlayer regions 12 formed on the stimulable phosphor sheet 10 upon beingstimulated using the first laser stimulating ray source 1 and has aproperty of transmitting only light having a wavelength corresponding tothat of stimulated emission emitted from stimulable phosphor but cuttingoff light having a wavelength of 640 nm.

[0279] Therefore, in accordance with the kind of a stimulating raysource to be used, one of these filter members 51 a, 51 b, 51 c, 51 d isselectively positioned in front of the photomultiplier 50, therebyenabling the photomultiplier 50 to photoelectrically detect only lightto be detected.

[0280] The analog data produced by photoelectrically detecting lightwith the photomultiplier 50 are converted by an A/D converter 53 intodigital data and the digital data are fed to a data processing apparatus54.

[0281] Although not shown in FIG. 6, the optical head 35 is constitutedto be movable by a scanning mechanism in the X direction and the Ydirection in FIG. 6 so that all of the dot-like stimulable phosphorlayer regions 12 formed on the stimulable phosphor sheet 10 or the wholesurface of the biochemical analysis unit 1 can be scanned by the laserbeam 24.

[0282]FIG. 12 is a schematic plan view showing the scanning mechanism ofthe optical head 35. In FIG. 12, optical systems other than the opticalhead 35 and the paths of the laser beam 24 and stimulated emission 45 orfluorescence 45 are omitted for simplification.

[0283] As shown in FIG. 12, the scanning mechanism of the optical head35 includes a base plate 60, and a sub-scanning pulse motor 61 and apair of rails 62, 62 are fixed on the base plate 60. A movable baseplate 63 is further provided so as to be movable in the sub-scanningdirection indicated by an arrow Y in FIG. 12.

[0284] The movable base plate 63 is formed with a threaded hole (notshown) and a threaded rod 64 rotated by the sub-scanning pulse motor 61is engaged with the inside of the hole.

[0285] A main scanning pulse motor 65 is provided on the movable baseplate 63. The main scanning pulse motor 65 is adapted for driving anendless belt 66. The optical head 35 is fixed to the endless belt 66 andwhen the endless belt 66 is driven by the main scanning pulse motor 65,the optical head 35 is moved in the main scanning direction indicated byan arrow X in FIG. 12. In FIG. 12, the reference numeral 67 designates alinear encoder for detecting the position of the optical head 35 in themain scanning direction and the reference numeral 68 designates slits ofthe linear encoder 67.

[0286] Therefore, the optical head 35 is moved in the X direction andthe Y direction in FIG. 12 by driving the endless belt 66 in the mainscanning direction by the main scanning pulse motor 65 and moving themovable base plate 63 in the sub-scanning direction by the sub-scanningpulse motor 61, thereby scanning all of the dot-like stimulable phosphorlayer regions 12 formed on the stimulable phosphor sheet 10 or the wholesurface of the biochemical analysis unit 1 with the laser beam 24.

[0287]FIG. 13 is a block diagram of a control system, an input systemand a drive system of the scanner shown in FIG. 6.

[0288] As shown in FIG. 13, the control system of the scanner includes acontrol unit 70 for controlling the whole operation of the scanner andthe input system of the scanner includes a keyboard 71 which can beoperated by an operator and through which various instruction signalscan be input.

[0289] As shown in FIG. 13, the drive system of the scanner includes afilter unit motor 72 for moving the filter unit 48 provided with thefour filter members 51 a, 51 b, 51 c and 51 d.

[0290] The control unit 70 is adapted for selectively outputting a drivesignal to the first laser stimulating ray source 21, the second laserstimulating ray source 22 or the third laser stimulating ray source 23and outputting a drive signal to the filter unit motor 72.

[0291] The thus constituted scanner reads fluorescence data of afluorescent substance such as a fluorescent dye carried in the porousmaterial 4 charged in a number of through-holes 3 formed in thebiochemical analysis unit 1 and produces biochemical analysis data inthe following manner.

[0292] A biochemical analysis unit 1 is first set on the glass plate 41of the stage 40 by an operator.

[0293] The kind of fluorescent substance as a labeling substance is theninput through the keyboard 71 by the operator and an instruction signalindicating that fluorescence data are to be read is input through thekeyboard 71.

[0294] The instruction signal is input to the control unit 70 and whenthe control unit 70 receives it, it determines the laser stimulating raysource to be used in accordance with a table stored in a memory (notshown) and also determines what filter is to be positioned in theoptical path of fluorescence 45 among the filters 52 a, 52 b and 52 c.

[0295] For example, when Rhodamine (registered trademark), which can bemost efficiently stimulated by a laser beam having a wavelength of 532nm, is used as a fluorescent substance for labeling a substance derivedfrom a living organism and a signal indicating such a fact is input, thecontrol unit 70 selects the second laser stimulating ray source 22 andthe filter 52 b and outputs a drive signal to the filter unit motor 72,thereby moving the filter unit 48 so that the filter member 51 binserting the filter 52 b having a property of cutting off light havinga wavelength of 532 nm but transmitting light-having a wavelength longerthan 532 nm in the optical path of the fluorescence 45.

[0296] The control unit 70 then outputs a drive signal to the secondlaser stimulating ray source 22 to activate it, thereby causing it toemit a laser beam 24 having a wavelength of 532 nm.

[0297] The laser beam 24 emitted from the second laser stimulating raysource 22 is made a parallel beam by the collimator lens 30, advances tothe first dichroic mirror 27 and is reflected thereby.

[0298] The laser beam 24 reflected by the first dichroic mirror 27transmits through the second dichroic mirror 28 and enters the mirror29.

[0299] The laser beam 24 entering the mirror 29 is reflected by themirror 29 and further enters a mirror 32 to be reflected thereby.

[0300] The laser beam 24 reflected by the mirror 32 advances to theperforated mirror 34 and passes through the hole 33 of the perforatedmirror 34. Then, the laser beam 24 advances to the concave mirror 38.

[0301] The laser beam 24 advancing to the concave mirror 38 is reflectedthereby and enters the optical head 35.

[0302] The laser beam 24 entering the optical head 35 is reflected bythe mirror 36 and condensed by the aspherical lens 37 onto thebiochemical analysis unit 1 placed on the glass plate 41 of the stage40.

[0303] As a result, a fluorescent substance such as a fluorescent dye,for instance, Rhodamine, contained in the porous material 4 charged in anumber of through-holes 3 formed in the biochemical analysis unit 1 isstimulated by the laser beam 24 and fluorescence 45 is released fromRhodamine.

[0304] In the biochemical analysis unit 1 according to this embodiment,since the substrate 2 of the biochemical analysis unit 1 is formed of ametal having a property capable of attenuating radiation energy andlight energy, it is possible to reliably prevent fluorescence releasedfrom a fluorescent substance contained in porous material 4 charged in athrough-hole 3 from being scattered in the substrate 2 and mixed withfluorescent released from a fluorescent substance contained in porousmaterial 4 charged in through-holes 3 neighboring the through-hole 3.

[0305] The fluorescence 45 released from Rhodamine is condensed by theaspherical lens 37 provided in the optical head 35 and reflected by themirror 36 on the side of an optical path of the laser beam 24, therebybeing made a parallel beam to advance to the concave mirror 38.

[0306] The fluorescence 45 advancing to the concave mirror 38 isreflected by the concave mirror 38 and advances to the perforated mirror34.

[0307] As shown in FIG. 7, the fluorescence 45 advancing to theperforated mirror 34 is reflected downward by the perforated mirror 34formed as a concave mirror and advances to the filter 52 b of a filterunit 48.

[0308] Since the filter 52 b has a property of cutting off light havinga wavelength of 532 nm but transmitting light having a wavelength longerthan 532 nm, light having the same wavelength of 532 nm as that of thestimulating ray is cut off by the filter 52 b and only light in thewavelength of the fluorescence 45 released from Rhodamine passes throughthe filter 52 b to be photoelectrically detected by the photomultiplier50.

[0309] As described above, since the optical head 35 is moved on thebase plate 63 in the X direction in FIG. 12 by the main scanning pulsemotor 65 mounted on the base plate 63 and the base plate 63 is moved inthe Y direction in FIG. 12 by the sub-scanning pulse motor 61, the wholesurface of the biochemical analysis unit 1 is scanned by the laser beam24. Therefore, the photomultiplier 50 can read fluorescent data ofRhodamine recorded in the biochemical analysis unit 1 byphotoelectrically detecting the fluorescence 45 released from Rhodaminecontained in the porous material in a number of through-holes 3 andproduce analog data for biochemical analysis.

[0310] The analog data produced by photoelectrically detecting thestimulated emission 45 with the photomultiplier 50 are converted by theAID converter 53 into digital data and the digital data are fed to thedata processing apparatus 54.

[0311] On the other hand, when radiation data recorded in a stimulablephosphor sheet 10 by exposing the dot-like stimulable phosphor layerregions 12 to a radioactive labeling substance contained in the porousmaterial in a number of through-holes 3 formed in the biochemicalanalysis unit 1 are to be read to produce biochemical analysis data, thestimulable phosphor sheet 10 is placed on the glass plate 41 of thestage 40 in such a manner that the dot-like stimulable phosphor layerregions 12 come into contact with the glass plate 41.

[0312] An instruction signal indicating that radiation data recorded inthe dot-like stimulable phosphor layer regions 12 formed on thestimulable phosphor sheet 10 are to be read is then input through thekeyboard 71.

[0313] The instruction signal input through the keyboard 71 is input tothe control unit 70 and the control unit 70 outputs a drive signal tothe filter unit motor 72 in accordance with the instruction signal,thereby moving the filter unit 48 so as to locate the filter member 51 dprovided with the filter 52 d having a property of transmitting onlylight having a wavelength corresponding to that of stimulated emissionemitted from stimulable phosphor but cutting off light having awavelength of 640 nm in the optical path of stimulated emission 45.

[0314] The control unit 70 then outputs a drive signal to the firstlaser stimulating ray source 21 to activate it, thereby causing it toemit a laser beam 24 having a wavelength of 640 nm.

[0315] The laser beam 24 emitted from the first laser stimulating raysource 21 is made a parallel beam by the collimator lens 25 and-advancesto the mirror 26 to be reflected thereby.

[0316] The laser beam 24 reflected by the mirror 26 passes through thefirst dichroic mirror 27 and the second dichroic mirror 28 and advancesto the mirror 29.

[0317] The laser beam 24 advancing to the mirror 29 is reflected by themirror 29 and further advances to a mirror 32 to be reflected thereby.

[0318] The laser beam 24 reflected by the mirror 32 passes through thehole 33 of the perforated mirror 34 and advances to the concave mirror38.

[0319] The laser beam 24 advancing to the concave mirror 38 is reflectedthereby and enters the optical head 35.

[0320] The laser beam 24 entering the optical head 35 is reflected bythe mirror 36 and condensed by the aspherical lens 37 onto the dot-likestimulable phosphor layer region 12 of the stimulable phosphor sheet 10placed on the glass plate 41 of the stage 40.

[0321] As a result, a stimulable phosphor contained in the dot-likestimulable phosphor layer region 12 formed on the stimulable phosphorsheet 10 is stimulated by the laser beam 24 and stimulated emission 45is released from the stimulable phosphor.

[0322] The stimulated emission 45 released from the stimulable phosphorcontained in the dot-like stimulable phosphor layer region 12 iscondensed by the aspherical lens 37 provided in the optical head 35 andreflected by the mirror 36 on the side of an optical path of the laserbeam 24, thereby being made a parallel beam to advance to the concavemirror 38.

[0323] The stimulated emission 45 advancing to the concave mirror 38 isreflected by the concave mirror 38 and advances to the perforated mirror34.

[0324] As shown in FIG. 7, the stimulated emission 45 advancing to theperforated mirror 34 is reflected downward by the perforated mirror 34formed as a concave mirror and advances to the filter 52 d of a filterunit 48.

[0325] Since the filter 52 d has a property of transmitting only lighthaving a wavelength corresponding to that of stimulated emission emittedfrom stimulable phosphor but cutting off light having a wavelength of640 nm, light having a wavelength of 640 nm corresponding to that of thestimulating ray is cut off by the filter 52 d and only light having awavelength corresponding to that of stimulated emission passes throughthe filter 52 d to be photoelectrically detected by the photomultiplier50.

[0326] As described above, since the optical head 35 is moved on thebase plate 63 in the X direction in FIG. 12 by the main scanning pulsemotor 65 mounted on the base plate 63 and the base plate 63 is moved inthe Y direction in FIG. 12 by the sub-scanning pulse motor 61, all ofthe dot-like stimulable phosphor layer regions 12 formed on thestimulable phosphor sheet 10 are scanned by the laser beam 24.Therefore, the photomultiplier 50 can read radiation data of aradioactive labeling substance recorded in a number of the dot-likestimulable phosphor layer regions 12 by photoelectrically detecting thestimulated emission 45 released from stimulable phosphor contained inthe stimulable phosphor layer regions l2and produce analog data.

[0327] The analog data produced by photoelectrically detecting thestimulated emission 45 with the photomultiplier 50 are converted by theA/D converter 53 into digital data and the digital data are fed to thedata processing apparatus 54.

[0328]FIG. 14 is a schematic front view showing a data producing systemfor reading chemiluminescent data of a labeling substance recorded inabsorptive regions formed in a number of through-holes 3 formed in thebiochemical analysis unit 1, which generates chemiluminescent emissionwhen it contacts a chemiluminescent substrate and producing biochemicalanalysis data. The data producing system shown in FIG. 14 is constitutedto be able to also read fluorescence data of a fluorescent substancesuch as a fluorescent dye recorded in a porous material 4 charged in anumber of through-holes 3 formed in the biochemical analysis unit 1.

[0329] As shown in FIG. 14, the data producing system includes a cooledCCD camera 81, a dark box 82 and a personal computer 83. As shown inFIG. 14, the personal computer 83 is equipped with a CRT 84 and akeyboard 85.

[0330]FIG. 15 is a schematic longitudinal cross sectional view showingthe cooled CCD camera 81.

[0331] As shown in FIG. 15, the cooled CCD camera 81 includes a CCD 86,a heat transfer plate 87 made of metal such as aluminum, a Peltierelement 88 for cooling the CCD 86, a shutter 89 disposed in front of theCCD 86, an A/D converter 90 for converting analog data produced by theCCD 86 to digital data, a data buffer 91 for temporarily storing thedata digitized by the A/D converter 90, and a camera control circuit 92for controlling the operation of the cooled CCD camera 81. An openingformed between the dark box 82 and the cooled CCD camera 81 is closed bya glass plate 95 and the periphery of the cooled CCD camera 81 is formedwith heat dispersion fins 96 over substantially half its length fordispersing heat.

[0332] A camera lens 97 disposed in the dark box 82 is mounted on thefront surface of the glass plate 95 disposed in the cooled CCD camera81.

[0333]FIG. 16 is a schematic vertical cross sectional view showing thedark box 82.

[0334] As shown in FIG. 16, the dark box 82 is equipped with a lightemitting diode stimulating ray source 100 for emitting a stimulatingray. The light emitting diode stimulating ray source 100 is providedwith a filter 101 detachably mounted thereon and a diffusion plate 102mounted on the upper surface of the filter 101. The stimulating ray isemitted via the diffusion plate 102 toward a biochemical analysis unit(not shown) placed on the diffusion plate 102 so as to ensure that thebiochemical analysis unit can be uniformly irradiated with thestimulating ray. The filter 101 has a property of cutting lightcomponents having a wavelength not close to that of the stimulating rayand harmful to the stimulation of a fluorescent substance andtransmitting through only light components having a wavelength in thevicinity of that of the stimulating ray. A filter 102 for cutting lightcomponents having a wavelength in the vicinity of that of thestimulating ray is detachably provided on the front surface of thecamera lens 97.

[0335]FIG. 17 is a block diagram of the personal computer 83 andperipheral devices thereof.

[0336] As shown in FIG. 17, the personal computer 83 includes a CPU 110for controlling the exposure of the cooled CCD camera 81, a datatransferring means 111 for reading the data produced by the cooled CCDcamera 81 from the data buffer 91, a storing means 112 for storing data,a data processing apparatus 113 for effecting data processing on thedigital data stored in the data storing means 112, and a data displayingmeans 114 for displaying visual data on the screen of the CRT 84 basedon the digital data stored in the data storing means 112. The lightemitting diode stimulating ray source 100 is controlled by a lightsource control means 115 and an instruction signal can be input via theCPU 110 to the light source control means 115 through the keyboard 85.The CPU 110 is constituted so as to output various signals to the cameracontrolling circuit 93 of the cooled CCD camera 81.

[0337] The data producing system shown in FIGS. 14 to 17 is constitutedso as to detect chemiluminescent emission generated by the contact of alabeling substance contained in the porous material 4 charged in anumber of through-holes 3 formed in the biochemical analysis unit 1 anda chemiluminescent substrate, with the CCD 86 of the cooled CCD camera81 through a camera lens 97, thereby producing chemiluminescence data,and irradiate the biochemical analysis unit 1 with a stimulating rayemitted from the light emitting diode stimulating ray source 100 anddetect fluorescence released from a fluorescent substance such as afluorescent dye contained in the porous material 4 charged in a numberof through-holes 3 formed in the biochemical analysis unit 1 upon beingstimulated, with the CCD 86 of the cooled CCD camera 81 through a cameralens 97, thereby producing fluorescence data.

[0338] When chemiluminescence data are to be read out, the filter 102 isremoved and while the light emitting diode stimulating ray source 100 iskept off, the biochemical analysis unit 1 is placed on the diffusionplate 103, which is releasing chemiluminescent emission as a result ofcontact of a labeling substance contained in the porous material 4charged in a number of through-holes 3 formed in the biochemicalanalysis unit 1 and a chemiluminescent substrate.

[0339] The lens focus is then adjusted by an operator using the cameralens 97 and the dark box 92 is closed.

[0340] When an exposure start signal is input by the operator throughthe keyboard 85, the exposure start signal is input through the CPU 110to the camera control circuit 92 of the cooled CCD camera 81 so that theshutter 88 is opened by the camera control circuit 92, whereby theexposure of the CCD 86 is started.

[0341] Chemiluminescent emission released from the biochemical analysisunit 1 impinges on the light receiving surface of the CCD 86 of thecooled CCD camera 81 via the camera lens 97, thereby forming an image onthe light receiving surface. The CCD 86 receives light of the thusformed image and accumulates it in the form of electric charges therein.

[0342] In this embodiment, since the substrate 2 of the biochemicalanalysis unit 1 is formed of a metal capable of attenuating radiationenergy and light energy, it is possible to reliably preventchemiluminescent emission released from the labeling substance frombeing scattered in the substrate 2 and mixed with chemiluminescentemission released from a labeling substance contained in porous material4 charged in neighboring through-holes 3.

[0343] When a predetermined exposure time has passed, the CPU 110outputs an exposure completion signal to the camera control circuit 92of the cooled CCD camera 81.

[0344] When the camera controlling circuit 92 receives the exposurecompletion signal from the CPU 110, it transfers analog data accumulatedin the CCD 86 in the form of electric charge to the A/D converter 90 tocause the A/D converter 90 to digitize the data and to temporarily storethe thus digitized data in the data buffer 91.

[0345] At the same time, the CPU 110 outputs a data transfer signal tothe data transferring means 111 to cause it to read out the digital datafrom the data buffer 91 of the cooled CCD camera 81 and to input them tothe data storing means 112.

[0346] When the operator inputs a data producing signal through thekeyboard 85, the CPU 110 outputs the digital data stored in the datastoring means 112 to the data processing apparatus 113 and causes thedata processing apparatus 113 to effect data processing on the digitaldata in accordance with the operator's instructions. The CPU 110 thenoutputs a data display signal to the displaying means 115 and causes thedisplaying means 115 to display biochemical analysis data on the screenof the CRT 84 based on the thus processed digital data.

[0347] On the other hand, when fluorescence data are to be read out, thebiochemical analysis unit 1 is first placed on the diffusion plate 103.

[0348] The light emitting diode stimulating ray source 100 is thenturned on by the operator and the lens focus is adjusted using thecamera lens 97. The dark box 92 is then closed.

[0349] When the operator inputs an exposure start signal through thekeyboard 85, the light emitting diode stimulating ray source 100 isagain turned on by the light source control means 115, thereby emittinga stimulating ray toward the biochemical analysis unit 1. At the sametime, the exposure start signal is input via the CPU 110 to the cameracontrol circuit 92 of the cooled CCD camera 81 and the shutter 89 isopened by the camera control circuit 92, whereby the exposure of the CCD86 is started.

[0350] The stimulating ray emitted from the light emitting diodestimulating ray source 100 passes through the filter 101, whereby lightcomponents of wavelengths not in the vicinity of that of the stimulatingray are cut. The stimulating ray then passes through the diffusion plate103 to be made uniform light and the biochemical analysis unit 1 isirradiated with the uniform stimulating ray.

[0351] The fluorescence released from the biochemical analysis unit 1impinges on the light receiving surface of the CCD 86 of the cooled CCDcamera 81 through the filter 102 and the camera lens 97 and forms animage thereon. The CCD 86 receives light of the thus formed image andaccumulates it in the form of electric charges therein. Since lightcomponents of wavelength equal to the stimulating ray wavelength are cutby the filter 102, only fluorescence released from the fluorescentsubstance contained in the porous material 4 charged in a number of thethrough-holes 3 formed in the biochemical analysis unit 1 is received bythe CCD 86.

[0352] In this embodiment, since the substrate 2 of the biochemicalanalysis unit 1 is formed of a metal capable of attenuating radiationenergy and light energy, it is possible to reliably prevent fluorescencereleased from the fluorescent substance such as a fluorescent dye frombeing scattered in the substrate 2 and mixed with fluorescence releasedfrom a fluorescent substance contained in porous material 4 charged inneighboring through-holes 3.

[0353] When a predetermined exposure time has passed, the CPU 110outputs an exposure completion signal to the camera control circuit 92of the cooled CCD camera 81.

[0354] When the camera controlling circuit 92 receives the exposurecompletion signal from the CPU 110, it transfers analog data accumulatedin the CCD 86 in the form of electric charge to the A/D converter 90 tocause the AID converter 90 to digitize the data and to temporarily storethe thus digitized data in the data buffer 91.

[0355] At the same time, the CPU 110 outputs a data transfer signal tothe data transferring means 111 to cause it to read out the digital datafrom the data buffer 91 of the cooled CCD camera 81 and to input them tothe data storing means 112.

[0356] When the operator inputs a data producing signal through thekeyboard 85, the CPU 110 outputs the digital data stored in the datastoring means 112 to the data processing apparatus 113 and causes thedata processing apparatus 113 to effect data processing on the digitaldata in accordance with the operator's instructions. The CPU 110 thenoutputs a data display signal to the displaying means 115 and causes thedisplaying means 115 to display biochemical analysis data on the screenof the CRT 84 based on the thus processed digital data.

[0357] In this embodiment, the biochemical analysis unit 1 includes thesubstrate 2 made of a metal capable of attenuating radiation energy andlight energy and having flexibility formed with a number of thethrough-holes 3, and the porous material 4 is charged in thethrough-holes 3. Specific binding substances such as a plurality ofcDNAs whose sequences are known but are different from each other arespotted into in a number of the through-holes 3 of the biochemicalanalysis unit 1 using the spotting device and are held by the porousmaterial 4.

[0358] A hybridization solution 9 containing a substance derived from aliving organism labeled with a radioactive labeling substance, asubstance derived from a living organism labeled with a labelingsubstance which generates chemiluminescent emission when it contacts achemiluminescent substrate and a substance derived from a livingorganism labeled with a fluorescent substance such as a fluorescent dyeis prepared and the biochemical analysis unit 1 is accommodated in thehybridization vessel 8 containing the thus prepared hybridizationsolution 9, whereby specific binding substances spotted in a number ofthe through-holes 3 charged with porous material 4 are hybridized withthe substances derived from a living organism contained in thehybridization solution 9 and the specific binding substances areselectively labeled with a radioactive labeling substance, a fluorescentsubstance such as a fluorescent dye and a labeling substance whichgenerates chemiluminescent emission when it contacts a chemiluminescentsubstrate.

[0359] When the stimulable phosphor sheet 10 is to be exposed to aradioactive labeling substance, the stimulable phosphor sheet 10including the support 11 on one side of which a number of dot-likestimulable phosphor layer regions 12 are formed in the same pattern asthat of a number of through-holes 3 formed in the biochemical analysisunit 1 is superposed on the biochemical analysis unit 1 in such a mannerthat each of the dot-like stimulable phosphor layer regions 12 formed inthe stimulable phosphor sheet 10 is located in one of the manythrough-holes 3 formed in the biochemical analysis unit 1 and that thesurface of each of the dot-like stimulable phosphor layer regions 12comes into close contact with the surface of the porous material 4charged in one of the through-holes 3, thereby exposing a number of thedot-like stimulable phosphor layer regions 12.

[0360] Therefore, according to this embodiment, since the substrate 2 ofthe biochemical analysis unit 1 is formed of a metal capable ofattenuating radiation energy and light energy, when the stimulablephosphor sheet 10 is to be exposed, electron beams released from theradioactive labeling substance are prevented from being scattered in thesubstrate 2. Further, since each of a number of dot-like stimulablephosphor layer regions 12 formed in the stimulable phosphor sheet 10 islocated in one of the many through-holes 3 formed in the biochemicalanalysis unit 1, the electron beams released from the radioactivelabeling substance are prevented from being scattered in the dot-likestimulable phosphor layer region 12 and advancing to dot-like stimulablephosphor layer regions 12 located in neighboring through-holes.Accordingly, even when the through-holes 3 are formed in the substrate 2at high density, it is possible to reliably expose a number of thedot-like stimulable phosphor layer regions 12 formed in the stimulablephosphor sheet 10 to only the radioactive labeling substance containedin the porous material 4 charged in the corresponding through-holes 3.

[0361] Furthermore, according to this embodiment, since the substrate 2of the biochemical analysis unit 1 is formed of a metal capable ofattenuating radiation energy and light energy, fluorescence releasedfrom a fluorescent substance such as a fluorescent dye as a result ofbeing irradiated with a laser beam 24 or a stimulating ray emitted fromthe light emitting diode stimulating ray source 100, can be reliablyprevented from being scattered in the substrate 2 and mixed withfluorescence released from a fluorescent substance such as a fluorescentdye contained in porous material 4 charged in neighboring through-holes3. Therefore, even when the through-holes 3 are formed in the substrate2 at high density, it is possible to reliably prevent noise caused bythe scattering of fluorescence from being generated in biochemicalanalysis data produced by photoelectrically detecting fluorescence andimprove the quantitative accuracy of biochemical analysis.

[0362] Furthermore, according to this embodiment, since the substrate 2of the biochemical analysis unit 1 is formed of a metal capable ofattenuating radiation energy and light energy, chemiluminescent emissionreleased a labeling substance by the contact with a chemiluminescentsubstrate can be reliably prevented from being scattered in thesubstrate 2 and mixed with chemiluminescent emission released from alabeling substance contained in porous material 4 charged in neighboringthrough-holes 3. Therefore, even when the through-holes 3 are formed inthe substrate 2 at high density, it is possible to reliably preventnoise caused by the scattering of chemiluminescent emission from beinggenerated in biochemical analysis data produced by photoelectricallydetecting chemiluminescent emission and improve the quantitativeaccuracy of biochemical analysis.

[0363] Further, according to this embodiment, since the substrate 2 ofthe biochemical analysis unit 1 is formed of a metal having flexibility,the biochemical analysis unit 1 can be bent and accommodated in thehybridization vessel 8 so as to be aligned with the inner wall surfacethereof, whereby specific binding substances are selectively hybridizedwith substances derived from a living organism. Therefore, hybridizationcan be accomplished using a small amount of the hybridization solution9.

[0364] Furthermore, according to this embodiment, since the substrate 2of the biochemical analysis unit 1 is formed of a metal, it is hardlystretched and shrunk even when it is subjected to liquid processing suchas hybridization and, therefore, it is possible to easily and accuratelysuperpose the stimulable phosphor sheet 10 on the biochemical analysisunit 1 so that each of the dot-like stimulable phosphor layer regions 12formed in the stimulable phosphor sheet 10 is located in one of the manythrough-holes 3 formed in the biochemical analysis unit 1 and that thesurface of each of the dot-like stimulable phosphor layer regions 12comes into close contact with the surface of the porous material 4charged in one of the through-holes 3, thereby exposing the dot-likestimulable phosphor layer regions 12.

[0365]FIG. 18 is a schematic vertical cross sectional view showinganother example of a dark box.

[0366] As shown in FIG. 18, at the bottom of a dark box 82 according tothis embodiment, a vessel 131 containing a solution 130 containing achemiluminescent substrate is provided and the inner wall surface of thevessel 131 is formed with support members 132 for supporting thebiochemical analysis unit 1.

[0367] When chemiluminescence data are to be read out, it is preferablefor improving the quantitative accuracy to keep the labeling substancecontained in the porous material 4 charged in a number of thethrough-holes 3 of the biochemical analysis unit 1 and thechemiluminescent substrate constantly in contact with each other so asto cause release of chemiluminescent emission having a predeterminedintensity. Therefore, in the dark box 82 according to this embodiment,since the support members 132 enable the biochemical analysis unit 1tobe kept constantly in contact with the solution 130 containing achemiluminescent substrate accommodated in the vessel 131 provided atthe bottom of the dark box 82 and chemiluminescent emission can bedetected by the cooled CCD camera 81, it is possible to markedly improvethe quantitative accuracy of biochemical analysis.

[0368]FIG. 19 is a schematic longitudinal cross sectional view showing abiochemical analysis unit which is another embodiment of the presentinvention.

[0369] As shown in FIG. 19, a biochemical analysis unit 1 includes anabsorptive substrate 140 formed of absorptive material such as nylon-6and is formed by closely contacting perforated plates 142, 142 made of ametal capable of attenuating radiation energy and light energy andhaving flexibility and formed with a number of through-holes 141.

[0370] Although not accurately shown in FIG. 19, in this embodiment,similarly to the substrate 2 according to the previous embodiment, about10,000 through-holes 141 having a size of about 0.01 cm² are regularlyformed at a density of about 10,000 per cm² in the perforated plates142, 142 and a number of absorptive regions 144 are formed by theabsorptive substrate 140 located in the through-holes 141.

[0371] In this embodiment, when biochemical analysis is to be performed,specific binding substances such as a plurality of cDNAs whose sequencesare known but are different from each other are spotted using thespotting device shown in FIG. 2 onto a number of the absorptive regions144 formed on the absorptive substrate 140 via a number of thethrough-holes 141 formed in the perforated plates 142, 142.

[0372] When hybridization is to be performed, similarly to the previousembodiment, the biochemical analysis unit 1 including a number of theabsorptive regions 144 into which specific binding substances have beenspotted is inserted into the hybridization vessel 7, whereby thespecific binding substances are selectively hybridized with a substancederived from a living organism labeled with a radioactive labelingsubstance, a substance derived from a living organism labeled with alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate and a substance derived from aliving organism labeled with a fluorescent substance such as afluorescent dye contained in the hybridization solution 9.

[0373] As a result of the hybridization, fluorescence data of afluorescent substance such as a fluorescent dye and chemiluminescencedata of a substance derived from a living organism labeled with alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate are recorded in the absorptiveregions 144 formed on the absorptive substrate 140.

[0374] When the stimulable phosphor sheet 10 is to be exposed to aradioactive labeling substance, as shown in FIG. 4, the stimulablephosphor sheet 10 formed with a number of dot-like stimulable phosphorlayer regions 12 is superposed on the biochemical analysis unit 1. Thedot-like stimulable phosphor layer regions 12 are formed in thestimulable phosphor sheet 10 in the same regular pattern as that of anumber of the through-holes 141 formed in the perforated plate 142.

[0375]FIG. 20 is a schematic cross-sectional view showing a method forexposing a number of the dot-like stimulable phosphor layer regions 12formed on the stimulable phosphor sheet 10 to a radioactive labelingsubstance contained in a number of the absorptive regions 144 formed onthe absorptive substrate 140.

[0376] As shown in FIG. 20, when the stimulable phosphor sheet 10 is tobe exposed, the stimulable phosphor sheet 10 is superposed on thebiochemical analysis unit 1 in such a manner that each of the dot-likestimulable phosphor layer regions 12 formed in the stimulable phosphorsheet 10 is located in one of the through-holes 3 formed in one of theperforated plates 142 of the biochemical analysis unit 1 and that thesurface of each of the dot-like stimulable phosphor layer regions 12comes into close contact with the surface of the absorptive region 144.

[0377] Since specific binding substances are spotted using the spottingdevice on the absorptive regions 144 formed on the absorptive substrate140 via the perforated plate 142, the surface of each of the dot-likestimulable phosphor layer regions 12 is accurately located in closecontact with the spot-like regions formed on the surface of theabsorptive substrate 140 and selectively labeled with a radioactivelabeling substance.

[0378] In this manner, the surface of each of the dot-like stimulablephosphor layer regions 12 is kept in close contact with the surface ofthe absorptive regions 144 formed on the absorptive substrate 140 for apredetermined time period, whereby a number of the dot-like stimulablephosphor layer regions 12 formed in the stimulable phosphor sheet 10 areexposed to the radioactive labeling substance contained in a number ofthe absorptive regions 144.

[0379] During the exposure operation, electron beams are released fromthe radioactive labeling substance. However, since the perforated plate140 is formed of a metal capable of attenuating radiation energy andlight energy, electron beams released from the radioactive labelingsubstance contained in the individual absorptive regions 144 formed onthe absorptive substrate 140 are prevented from being mixed withelectron beams released from the radioactive labeling substancecontained in neighboring absorptive regions 144 formed on the absorptivesubstrate 140. Further, since each of a number of dot-like stimulablephosphor layer regions 12 formed in the stimulable phosphor sheet 10 islocated in one of the through-holes 141 formed in the perforated plate142, the electron beams released from the radioactive labeling substanceare reliably prevented from being scattered in the dot-like stimulablephosphor layer region 12 and advancing to the dot-like stimulablephosphor layer regions 12 located in neighboring through-holes 141.Therefore, it is possible to reliably expose a number of the dot-likestimulable phosphor layer regions 12 formed in the stimulable phosphorsheet 10 to only the radioactive labeling substance contained in theabsorptive regions 144 formed on the absorptive substrate 140 viacorresponding through-holes 141 of the perforated plate 142.

[0380] In this manner, radiation data of a radioactive labelingsubstance are recorded in a number of the dot-like stimulable phosphorlayer regions 12 formed in the stimulable phosphor sheet 10.

[0381] Therefore, in the case where biochemical analysis data areproduced by irradiating the dot-like stimulable phosphor layer regions12 formed in the support 11 of the stimulable phosphor sheet 10 at highdensity and exposed to a radioactive labeling substance with astimulating ray and photoelectrically detecting stimulated emissionreleased from the dot-like stimulable phosphor layer regions 12, andsubstances derived from a living organism are analyzed, it is possibleto effectively prevent noise caused by the scattering of electron beamsreleased from the radioactive labeling substance from being generated inbiochemical analysis data.

[0382] On the other hand, chemiluminescence data of a labeling substancewhich generates chemiluminescent emission when it contacts achemiluminescent substrate or fluorescence data of a fluorescentsubstance such as a fluorescent dye recorded in a number of theabsorptive regions 144 formed on the absorptive substrate 140 are readout by the data producing system shown in FIGS. 14 to 17, therebyproducing biochemical analysis data.

[0383] Since the perforate plate 142 formed with a number of thethrough-holes 141 is located on the side of the camera lens 97 withrespect to the absorptive substrate 140 so as to be in close contactwith the absorptive substrate 140, chemiluminescent emission releasedfrom a labeling substance which generates chemiluminescent emission whenit contacts a chemiluminescent substrate or fluorescence released from afluorescent substance contained in the individual absorptive regions 144can be reliably prevented from being mixed with chemiluminescentemission released from the labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateor fluorescence released from the fluorescent substance contained inneighboring absorptive regions 144 formed on the absorptive substrate140 and, therefore, it is possible to effectively prevent noise causedby the scattering of chemiluminescent emission or fluorescence frombeing generated in biochemical analysis data produced byphotoelectrically detecting chemiluminescent emission or fluorescence.

[0384] According to this embodiment, since the perforated plate 142 ismade of a metal capable of attenuating radiation energy and lightenergy, electron beams released from the radioactive labeling substancecontained in the individual absorptive regions 144 formed on theabsorptive substrate 140 can be reliably prevented from being mixed withelectron beams released from the radioactive labeling substancecontained in neighboring absorptive regions 144 formed on the absorptivesubstrate 140 and since each of a number of dot-like stimulable phosphorlayer regions 12 formed in the stimulable phosphor sheet 10 is locatedin one of the through-holes 141 formed in the biochemical analysis unit1, electron beams released from the radioactive labeling substance areprevented from being scattered in the dot-like stimulable phosphor layerregion 12 and advancing to the dot-like stimulable phosphor layerregions 12 located in neighboring through-holes 141. Accordingly, it ispossible to reliably expose a number of the dot-like stimulable phosphorlayer regions 12 formed in the stimulable phosphor sheet 10 to only theradioactive labeling substance contained in the corresponding absorptiveregions 144 formed on the absorptive substrate 140 via the correspondingthrough-holes 141 of the perforated plate 142. Therefore, in the casewhere biochemical analysis data are produced by irradiating the dot-likestimulable phosphor layer regions 12 formed in the support 11 of thestimulable phosphor sheet 10 at high density and exposed to aradioactive labeling substance with a stimulating ray andphotoelectrically detecting stimulated emission released from thedot-like stimulable phosphor layer regions 12, and substances derivedfrom a living organism are analyzed, it is possible to effectivelyprevent noise caused by the scattering of electron beams released fromthe radioactive labeling substance from being generated in biochemicalanalysis data.

[0385] On the other hand, according to this embodiment, since theperforate plate 142 formed with a number of the through-holes 141 islocated on the side of the camera lens 97 with respect to the absorptivesubstrate 140 so as to be in close contact with the absorptive substrate140, chemiluminescent emission released from a labeling substance whichgenerates chemiluminescent emission when it contacts a chemiluminescentsubstrate or fluorescence released from a fluorescent substancecontained the individual absorptive regions 144 formed on the absorptivesubstrate 140 can be reliably prevented from being mixed withchemiluminescent emission released from the labeling substance whichgenerates chemiluminescent emission when it contacts a chemiluminescentsubstrate or fluorescence released from the fluorescent substancecontained in neighboring absorptive regions 144 formed on the absorptivesubstrate 140 and, therefore, it is possible to effectively preventnoise caused by the scattering of chemiluminescent emission orfluorescence from being generated in biochemical analysis data producedby photoelectrically detecting chemiluminescent emission orfluorescence.

[0386]FIG. 21 is a schematic perspective view of a biochemical analysisunit which is a further preferred embodiment of the present invention.

[0387] A biochemical analysis unit 151 shown in FIG. 21 includes anabsorptive substrate 152 made of absorptive material such as nylon-6 anda perforated plate 154 made of a metal such as aluminum and formed witha number of substantially circular through-holes 153 regularly and at ahigh density, and the absorptive substrate 152 and the perforated plate154 are in close contact with each other.

[0388] Although not accurately shown in FIG. 21, similarly to thebiochemical analysis unit shown in FIG. 19, in this embodiment, about10,000 through-holes 153 having a size of about 0.01 mm² are regularlyformed at a density of about 5,000 per cm² in the perforated plate 154and a number of absorptive regions 155 are regularly formed by theabsorptive substrate 152 in every through-holes 153.

[0389] As shown in FIG. 21, the perforated plate 154 is formed with agripping portion 156 in this embodiment.

[0390] As shown in FIG. 21, the perforated plate 154 of the biochemicalanalysis unit 151 according to this embodiment is formed with a pair ofpositioning through-holes 157, 158 in the vicinity of one side portion.

[0391]FIG. 22 is a schematic plan view showing another example of apotting device.

[0392] As shown in FIG. 22, a spotting device according to thisembodiment is provided with a drive mechanism and the drive mechanism ofthe spotting device is mounted on a frame 161 fixed to a base plate 160on which the biochemical analysis unit 151, onto which specific bindingsubstances such as cDNA are to be spotted, is to be set.

[0393] As shown in FIG. 22, a sub-scanning pulse motor 162 and a pair ofrails 163, 163 are fixed on the frame 161 and a movable base plate 164is further provided so as to be movable along the pair of rails 163, 163in the sub-scanning direction indicated by an arrow Y in FIG. 22.

[0394] The movable base plate 164 is formed with a threaded hole (notshown) and a threaded rod 165 rotated by the sub-scanning pulse motor162 is engaged with the inside of the hole.

[0395] A main scanning pulse motor 166 is provided on the movable baseplate 164. The main scanning pulse motor 165 is adapted forintermittently driving an endless belt 167 at a predetermined pitch.

[0396] The spotting head 5 of the spotting device is fixed to theendless belt 167 and when the endless belt 167 is driven by the mainscanning pulse motor 166, the spotting head 5 is moved in the mainscanning direction indicated by an arrow X in FIG. 22.

[0397] Although not shown in FIG. 22, the spotting head 5 includes aninjector 6 for ejecting a solution of specific binding substances towardthe biochemical analysis unit 151 and a CCD camera 7.

[0398] In FIG. 22, the reference numeral 168 designates a linear encoderfor detecting the position of the spotting head 5 in the main scanningdirection and the reference numeral 169 designates slits of the linearencoder 168.

[0399] As shown in FIG. 22, two positioning pins 177, 178 are uprightlyformed on the base plate 160 of the spotting device at positionscorresponding to those of the two positioning through-holes 157, 158formed in the perforated plate 154 of the biochemical analysis unit 151.Placement of the biochemical analysis unit 151 at a substantiallyconstant position on the base plate 160 of the spotting device isensured by placing the biochemical analysis unit 151 on the base plate160 of the spotting device so that the two positioning pins 177, 178formed on the base plate 160 of the spotting device are inserted intothe corresponding positioning through-holes 157, 158.

[0400]FIG. 23 is a block diagram showing a control system, an inputsystem, a drive system and a detection system of the spotting device.

[0401] As shown in FIG. 23, the control system of the spotting deviceincludes a control unit 180 for controlling the whole operation of thespotting device and the input system of the spotting device includes akeyboard 181.

[0402] The drive system of the spotting device includes a main scanningpulse motor 166 and a sub-scanning pulse motor 162, and the detectionsystem of the spotting device includes a linear encoder 166 fordetecting the position of the spotting head 5 in the main scanningdirection, a rotary encoder 170 for detecting the amount of rotation ofthe rod 165 and a CCD camera 7.

[0403] Specific binding substances such as cDNA are spotted by the thusconstituted spotting device onto a number of absorptive regions 155formed in the biochemical analysis unit 151 according to this embodimentin the following manner.

[0404] The biochemical analysis unit 151 is first placed on the baseplate 160 of the spotting device so that the two positioning pins 177,178 formed on the base plate 160 of the spotting device enter thecorresponding positioning through-holes 157, 158.

[0405] In this embodiment, it is ensured in this manner that thebiochemical analysis unit 151 is placed at a substantially constantposition on the base plate 160 of the spotting device. However, sinceeach of the absorptive regions has a size of only about 0.01 mm² in thisembodiment, it cannot be ensured that the centers of the absorptiveregions 155 of the biochemical analysis unit 151 thus placed on the baseplate 160 are exactly aligned with the main scanning direction and thesub-scanning direction of the spotting head 5.

[0406] Therefore, the spotting device according to this embodiment isconstituted so as to detect in advance the relative positionalrelationship between the position of the biochemical analysis unit 151placed on the base plate 160 and the positions of the spotting head 5 tobe moved in main scanning direction and the sub-scanning direction, andto move the spotting head 5 by the main scanning pulse motor 166 and thesub-scanning pulse motor 162 so that the injector 6 can accurately spotspecific binding substances onto the absorptive regions 155.

[0407] When a spotting operation start signal is input by a user throughthe keyboard 181 and the spotting operation start signal is input to thecontrol unit 180, the control unit 180 outputs a drive signal to themain scanning pulse motor 166, thereby moving the spotting head 5located at a reference position in the main scanning direction indicatedby an arrow X in FIG. 22 and then outputs a drive signal to thesub-scanning pulse motor 162, thereby moving the spotting head 5 in thesub-scanning direction indicated by an arrow Y in FIG. 22.

[0408] While the spotting head 5 is being moved in the main scanningdirection indicated by an arrow X and in the main scanning directionindicated by an arrow X, the control unit 180 monitors detection signalsinput from the CCD camera 7, thereby detecting four corner portions ofthe biochemical analysis unit 151, calculates coordinate values of thefour corner portions of the biochemical analysis unit 151 in acoordinate system whose origin is the reference position of the spottinghead 5, and stores them in a memory (not shown).

[0409] When the four corner portions of the biochemical analysis unit151 are detected and the coordinate values thereof are stored in thememory, the control unit 180 calculates coordinate values of therespective absorptive regions 155 formed in the biochemical analysisunit 151 based on the coordinate values of the four corner portions ofthe biochemical analysis unit 151 in the coordinate system whose originis the reference position of the spotting head 5 and stores them in thememory (not shown).

[0410] When the coordinate values of the respective absorptive regions155 formed in the biochemical analysis unit 151 have been calculated inthe coordinate system whose origin is the reference position of thespotting head 5 and stored in the memory, the control unit 180 outputsdrive signals to the main scanning pulse motor 166 and the sub-scanningpulse motor 162, thereby returning the spotting head 5 to the originalreference position.

[0411] In the case where specific binding substances ejected from theinjector 6 of the spotting head 5 are accurately spotted at the positionthe tip end portion of the injector 6 faces, specific binding substancescan be accurately spotted onto the respective absorptive regions 155formed in the biochemical analysis unit 151 by ejecting specific bindingsubstances from the injector 6 of the spotting head 5 in theabove-described manner based on the coordinate values of the respectiveabsorptive regions 155 of the biochemical analysis unit 151 in thecoordinate system determined so that the reference position of thespotting head 5 is the origin thereof. However, in the case wherespecific binding substances ejected from the injector 6 of the spottinghead 5 are spotted at a position deviating in the X direction and/or theY direction from the position the tip end portion of the injector 6faces, even if specific binding substances are ejected from the injector6 of the spotting head 5 in the above-described manner based on thecoordinate values of the respective absorptive regions 155 of thebiochemical analysis unit 151 in the coordinate system determined sothat the reference position of the spotting head 5 is the originthereof, it is impossible to accurately spot specific binding substancesonto the respective absorptive regions 155 formed in the biochemicalanalysis unit 151.

[0412] In view of the above, in this embodiment, specific bindingsubstances are ejected from the injector 6 of the spotting head 5returned to the reference position thereof toward the surface of thebiochemical analysis unit 151, whereby the position of the thus spottedspecific binding substances is detected by the CCD camera 7, and amountsof deviation from the position the tip end portion of the injector 6faces in the X direction and the Y direction are calculated by thecontrol unit 180 based on a detection signal of the CCD camera 7 and thecalculated amounts of deviation are stored in the memory.

[0413] More specifically, as shown in FIG. 24, specific bindingsubstances are ejected from the injector 6 of the spotting head 5located at the reference position thereof toward the surface of thebiochemical analysis unit 151 and the position of the thus spottedspecific binding substances is detected by the CCD camera 7. The controlunit 180 then calculates an amount of deviation δx in the X directionand an amount of deviation δy in the Y direction from the position O thetip end portion of the injector 6 faces based on a detection signal fromthe CCD camera and stores them in the memory.

[0414] Since the amount of deviation δx in the X direction and theamount of deviation δy in the Y direction of the position of spottedspecific binding substances from the position O the tip end portion ofthe injector 6 faces are inherent in the respective injector 6 of thespotting head 5, it follows that the position of spotted specificbinding substances ejected from the injector 6 toward the surface of thebiochemical analysis unit 151 when the spotting head 5 is located at aposition other than the reference position thereof deviates from theposition O the tip end portion of the injector 6 faces by δx in the Xdirection and by δy in the Y direction.

[0415] Then, based on the coordinate values of the four corner portionsof the biochemical analysis unit 151 and the coordinate values of therespective absorptive regions 155 formed in the biochemical analysisunit 151 in the coordinate system whose origin is the reference positionof the spotting head 5 and the amount of deviation δx in the X directionand the amount of deviation δy in the Y direction of the position ofspotted specific binding substances, the control unit 180 calculatesdrive pulses to be sent to the main scanning pulse motor 166 and thesub-scanning pulse motor 162 in order to move the spotting head 5 topositions where the tip end portion of the injector 6 of the spottinghead 5 faces the respective absorptive regions 155 and stores drivingpulse data in the memory.

[0416] In this embodiment, a number of absorptive regions 155 of thebiochemical analysis unit 151 are formed one in every through-hole 153regularly formed in the perforated plate 154. Therefore, the drivepulses to be sent to the main scanning pulse motor 166 and thesub-scanning pulse motor 162 in order to move the spotting head 5 to aposition where the tip end portion of the injector 6 of the spottingdevice faces the third absorptive region 155 to which specific bindingsubstances are to be spotted and from there to each successive positionwhere the tip end portion of the injector 6 of the spotting device facesan absorptive region 155 to which specific binding substances are to bespotted are equal to the drive pulses to be sent to the main scanningpulse motor 166 and the sub-scanning pulse motor 162 in order to movethe spotting head 5 from the position where the tip end portion of theinjector 6 of the spotting device faces the first absorptive region 155to which specific binding substances are to be spotted to the positionwhere the tip end portion of the injector 6 of the spotting device facesthe second absorptive region 155 to which specific binding substancesare to be spotted. Accordingly, it is sufficient to calculate drivepulses to be sent to the main scanning pulse motor 166 and thesub-scanning pulse motor 162 in order to move the spotting head 5 fromthe reference position of the spotting head 5 to the position where thetip end portion of the injector 6 of the spotting device faces the firstabsorptive region 155 to which specific binding substances are to bespotted, calculate drive pulses to be sent to the main scanning pulsemotor 166 and the sub-scanning pulse motor 162 in order to move thespotting head 5 from the position where the tip end portion of theinjector 6 of the spotting device faces the first absorptive region 155to which specific binding substances are to be spotted to the positionwhere the tip end portion of the injector 6 of the spotting device facesthe second absorptive region 155 to which specific binding substancesare to be spotted, and store the calculated drive pulse data in thememory.

[0417] When drive pulses to be sent to the main scanning pulse motor 166and the sub-scanning pulse motor 162 in order to move the spotting head5 to the position where the tip end portion of the injector 6 of thespotting device faces the respective absorptive regions 155 have beencalculated and drive pulse data have been stored in the memory, thecontrol unit 180 sends predetermined drive pulses to the main scanningpulse motor 166 and the sub-scanning pulse motor 162 based on the drivepulse data stored in the memory, thereby intermittently moving thespotting head 5. When the spotting head 5 has reached the positionswhere it faces the respective absorptive regions 155 formed in thebiochemical analysis unit 151, the control unit 180 outputs drive stopsignals to the main scanning pulse motor 166 and the sub-scanning pulsemotor 162, thereby stopping the spotting head 5 and outputs a spotsignal to the injector 6 of the spotting head 5, thereby causing it tospot specific binding substances.

[0418] In the case where the spotting head 5 is to be moved to theposition where the tip end portion of the injector 6 of the spottinghead 5 faces the second or a subsequent absorptive region 155 to whichspecific binding substances are to be spotted, the spotting head 5 ismoved at predetermined pitches in the main scanning direction indicatedby the arrow X and in the sub-scanning direction indicated by the arrowY.

[0419] The spotting head 5 is intermittently moved by the main scanningpulse motor 166 and the sub-scanning pulse motor 162 in this manner andspecific binding substances are successively spotted onto the absorptiveregions 155 formed in the biochemical analysis unit 151.

[0420] According to this embodiment, the position of the biochemicalanalysis unit 151 with respect to the spotting head 5 is detected inadvance by the CCD camera 7, the coordinate values of the respectiveabsorptive regions 155 are calculated by the control unit 180 using thereference position of the spotting head 5 as the origin of thecoordinate system, and the calculated coordinate values are stored inthe memory. Specific binding substances are ejected toward the surfaceof the biochemical analysis unit 151 from the injector 6 of the spottinghead 5 located at the reference position thereof and the position wherethe specific binding substances are spotted is detected by the CCDcamera 7, whereby the amount of deviation δx in the X direction and theamount of deviation δy in the Y direction of the position of the spottedspecific binding substances from the position O where the tip endportion of the injector 6 faces are calculated by the control unit 180and stored in the memory. The control unit 180 calculates, based onthese data, drive pulses to be sent to the main scanning pulse motor 166and the sub-scanning pulse motor 162 in order to move the spotting head5 to the position where the tip end portion of the injector 6 of thespotting device faces the respective absorptive regions 155 and storesthe drive pulse data in the memory. When specific binding substances areto be spotted, the control unit 180 sends predetermined drive pulses tothe main scanning pulse motor 166 and the sub-scanning pulse motor 162based on the drive pulse data stored in the memory. When the spottinghead 5 has reached the positions where it faces the respectiveabsorptive regions 155 formed in the biochemical analysis unit 151, thecontrol unit 180 outputs drive stop signals to the main scanning pulsemotor 166 and the sub-scanning pulse motor 162, thereby stopping thespotting head 5 and outputs a spot signal to the injector 6 of thespotting head 5, thereby causing it to specific binding substances.Therefore, even when the biochemical analysis unit 151 is not accuratelyset on the base plate 160 so as to have a predetermined positionalrelationship with the spotting device, specific binding substances suchas cDNA can be reliably spotted in the respective absorptive regions 155formed in the biochemical analysis unit 151.

[0421] Further, according to this embodiment, since the biochemicalanalysis unit 151 includes the perforated plate 154 made of aluminum andthe perforated plate 154 is formed with the gripping portion 156, thebiochemical analysis unit 151 can be very easily handled when specificbinding substances are spotted, during hybridization or during exposureoperation.

[0422] Furthermore, according to this embodiment, the biochemicalanalysis unit 151 and the stimulable phosphor sheet 10 can be desirablypositioned for exposure utilizing the two positioning through-holes 157,158 formed in the vicinity of one side portion of the perforated plate154.

[0423]FIG. 25 is a schematic perspective view of a biochemical analysisunit which is a further preferred embodiment of the present invention.

[0424] Similarly to the biochemical analysis unit 1 shown in FIG. 1, abiochemical analysis unit 191 includes a substrate 192 made of aluminumand formed with a number of substantially circular through-holes 193regularly and at a high density, and a number of absorptive regions 194are formed by charging absorptive material such as nylon-6 in everythrough-hole 193.

[0425] The biochemical analysis unit 191 further includes a frame member196 including a pair of plate-like members 195, 195 and adapted forholding the peripheral portion of the substrate 192 therebetween andcarrying the substrate 192. The plate-like members 195, 195 are formedof rigid material.

[0426] Similarly to the embodiment shown in FIG. 21, as shown in FIG.25, the frame member 196 is formed with two positioning through-hole197, 198.

[0427] According to this embodiment, since the substrate 192 of thebiochemical analysis unit 191 is held between the frame member 196formed of rigid material, the biochemical analysis unit 191 can be veryeasily handled when specific binding substances are spotted, duringhybridization or during exposure operation.

[0428] Furthermore, according to this embodiment, since the frame member196 of the biochemical analysis unit 191 is formed with the twopositioning through-holes 197, 198, specific binding substances can beaccurately spotted onto a number of the absorptive regions 194 utilizingthe spotting device shown in FIG. 22.

[0429] Moreover, according to this embodiment, since the substrate 192of the biochemical analysis unit 191 is held between the frame member196 formed of rigid material, the biochemical analysis unit 191 and thestimulable phosphor sheet 10 can be desirably positioned for exposureutilizing the frame member 196 formed of rigid material.

[0430] The present invention has thus been shown and described withreference to specific embodiments. However, it should be noted that thepresent invention is in no way limited to the details of the describedarrangements but changes and modifications may be made without departingfrom the scope of the appended claims.

[0431] For example, in the above-described embodiments, as specificbinding substances, cDNAs each of which has a known base sequence and isdifferent from the others are used. However, specific binding substancesusable in the present invention are not limited to cDNAs but allspecific binding substances capable of specifically binding with asubstance derived from a living organism such as a hormone, tumormarker, enzyme, antibody, antigen, abzyme, other protein, a nuclearacid, cDNA, DNA, RNA or the like and whose sequence, base length,composition and the like are known, can be employed in the presentinvention as a specific binding substance.

[0432] Further, in the above-described embodiments, although thesubstrate 2 or the perforated plate 142 is made of a metal, it issufficient to make the substrate 2 or the perforated plate 142 of amaterial capable of attenuating radiation energy and light energy.Therefore, the invention is not limited to forming the substrate 2 orthe perforated plate 142 of a metal and the substrate 2 and theperforated plate 142 may instead be formed of a ceramic material or aplastic material.

[0433] Furthermore, in the above-described embodiments, although thesubstrate 2 or the perforated plate 142 has flexibility, it is notabsolutely necessary to form the substrate 2 or the perforated plate 142so as to be flexible.

[0434] Moreover, in the above-described embodiments, the substrate 2 orthe perforated plate 142 of the biochemical analysis unit 1 is made of amaterial capable of attenuating radiation energy and light energy.However, in the case where biochemical analysis is performed only bydetecting radiation data recorded in the dot-like stimulable phosphorlayer regions 12 of the stimulable phosphor sheet 10, the substrate 2 orthe perforated plate 142 may be made of a material capable oftransmitting light but attenuating radiation energy. On the other hand,in the case where biochemical analysis is performed only by detectingchemiluminescence data or fluorescence data, the substrate 2 or theperforated plate 142 may be made of a material capable of transmittingradiation but attenuating light energy. Therefore, it is not absolutelynecessary to form the substrate 2 or the perforated plate 142 of amaterial capable of attenuating radiation energy and light energy.

[0435] Further, a porous material is charged in a number of thethrough-holes 3 formed in the substrate 2 to form the absorptive regions4 in the embodiment shown in FIGS. 1 to 18. However, it is possible toform a number of recesses in the substrate 2, instead of thethrough-holes 3, and to charge or embed a porous material to form theabsorptive regions 4.

[0436] Furthermore, in the above-described embodiments, although about10,000 of the through-holes 3 or through-holes 143 having a size ofabout 0.01 cm² are regularly formed in the substrate 2 or the perforatedplate 142 at a density of about 10,000/cm², the number or size of thethrough-holes 3 or through-holes 143 may be arbitrarily selected inaccordance with the purposes. Preferably, 10 or more of thethrough-holes 3 or through-holes 143 having a size of 5 cm² or less areformed in the substrate 2 or the perforated plate 142 at a density of10/cm² or less.

[0437] Moreover, in the above-described embodiments, although about10,000 of the through-holes 3 or through-holes 143 having a size ofabout 0.01 cm² are regularly formed in the substrate 2 or the perforatedplate 142 at a density of about 10,000/cm², it is not absolutelynecessary to regularly form the through-holes 3 or through-holes 143 inthe substrate 2 or the perforated plate 142.

[0438] Further, in the above-described embodiments, a hybridizationsolution 9 containing a substance derived from a living organism labeledwith a radioactive labeling substance, a substance derived from a livingorganism labeled with a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand a substance derived from a living organism labeled with afluorescent substance such as a fluorescent dye is prepared andhybridized with specific binding substances spotted in the absorptiveregion 4. However, it is not absolutely necessary for substances derivedfrom a living organism to be labeled with a radioactive labelingsubstance, a fluorescent substance and a labeling substance whichgenerates chemiluminescent emission when it contacts a chemiluminescentsubstrate and it is sufficient for substances derived from a livingorganism to be labeled with at least one kind of a labeling substanceselected from a group consisting of a radioactive labeling substance, afluorescent substance and a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrate.

[0439] Furthermore, in the above-described embodiments, specific bindingsubstances are hybridized with substances derived from a living organismlabeled with a radioactive labeling substance, a fluorescent substanceand a labeling substance which generates chemiluminescent emission whenit contacts a chemiluminescent substrate. However, it is not absolutelynecessary to hybridize substances derived from a living organism withspecific binding substances and substances derived from a livingorganism may be specifically bound with specific binding substances bymeans of antigen-antibody reaction, receptor-ligand reaction or the likeinstead of hybridization.

[0440] Moreover, in the above-described embodiments, a number of thedot-like stimulable phosphor layer regions 12 are formed on one surfaceof the support 11 of the stimulable phosphor sheet 10 in the samepattern as that of a number of the through-holes 3 formed in thebiochemical analysis unit 1 of the same pattern as that of a number ofthe through-holes 141 formed in the perforated plate 142. However, it isnot absolutely necessary to form the dot-like stimulable phosphor layerregions 12 and a stimulable phosphor layer may be uniformly formed onone surface of the support 11 of the stimulable phosphor sheet 10.

[0441] Further, the dot-like stimulable phosphor layer regions 12 areexposed to a radioactive labeling substance by superposing thebiochemical analysis unit 1 and the stimulable phosphor sheet 10 so thatthe absorption regions 4 formed in the through-holes 4 of thebiochemical analysis unit 1 and the dot-like stimulable phosphor layerregions 12 of the stimulable phosphor sheet 10 are in close contact witheach other in the embodiment shown in FIGS. 1 to 18 and the dot-likestimulable phosphor layer regions 12 are exposed to a radioactivelabeling substance by superposing the biochemical analysis unit 1 andthe stimulable phosphor sheet 10 so that the absorption regions 144formed on the absorptive substrate 140 of the biochemical analysis unit1 and the dot-like stimulable phosphor layer regions 12 of thestimulable phosphor sheet 10 are in close contact with each other in theembodiment shown in FIGS. 19 and 20. However, it is sufficient for thedot-like stimulable phosphor layer regions 12 to be exposed to aradioactive labeling substance by superposing the biochemical analysisunit 1 and the stimulable phosphor sheet 10 so that the dot-likestimulable phosphor layer regions 12 of the stimulable phosphor sheet 10face the absorption regions 4 formed in the through-holes 4 of thebiochemical analysis unit 1 or the absorptive substrate 140 of thebiochemical analysis unit 1 and it is not absolutely necessary to exposethe dot-like stimulable phosphor layer regions 12 to a radioactivelabeling substance by keeping the dot-like stimulable phosphor layerregions 12 of the stimulable phosphor sheet 10 in close contact with theabsorption regions 4 formed in the through-holes 4 of the biochemicalanalysis unit 1 or the absorptive regions 144 formed on the absorptivesubstrate 140 of the biochemical analysis unit 1.

[0442] Moreover, in the above-described embodiments, although a numberof the dot-like stimulable phosphor layer regions 12 of the stimulablephosphor sheet 10 are formed on the surface of the support 11, it is notabsolutely necessary to form a number of the dot-like stimulablephosphor layer regions 12 on the surface of the support 11. A number ofdot-like stimulable phosphor layer regions 12 may be formed by forming anumber of through-holes in the support 11 and charging or embeddingstimulable phosphor into a number of the through-holes or forming anumber of recesses in the support 11 and charging or embeddingstimulable phosphor into a number of the recesses.

[0443] Furthermore, in the above-described embodiments, although anumber of the dot-like stimulable phosphor layer regions 12 of thestimulable phosphor sheet 10 are formed so that the surface thereof islocated above the surface of the support 11, a number of the dot-likestimulable phosphor layer regions 12 may be formed so that the surfacethereof is flush with the surface of the support 11 or that the surfacethereof is located below the surface of the support 11.

[0444] Moreover, in the above-described embodiments, although thesupport 11 of the stimulable phosphor sheet 10 is made of stainless, itis sufficient for the support 11 to be made of a material capable ofattenuating radiation energy and light energy and the support 11 can beformed of either inorganic compound material or organic compoundmaterial and is preferably formed of metal material, ceramic material orplastic material Illustrative examples of inorganic compound materialsinclude metals such as gold, silver, copper, zinc, aluminum, titanium,tantalum, chromium, steel, nickel cobalt, lead, tin, selenium and thelike; alloys such as brass, stainless, bronze and the like; siliconmaterials such as silicon, amorphous silicon, glass, quartz, siliconcarbide, silicon nitride and the like; metal oxides such as aluminumoxide, magnesium oxide, zirconium oxide and the like; and inorganicsalts such as tungsten carbide, calcium carbide, calcium sulfate,hydroxy apatite, gallium arsenide and the like. High molecular compoundsare preferably used as organic compound material and illustrativeexamples thereof include polyolefins such as polyethylene, polypropyleneand the like; acrylic resins such as polymethyl methacrylate,polybutylacrylate/polymethyl methacrylate copolymer and the like;polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride;polyvinylidene fluoride; polytetrafluoroethylene;polychlorotrifuluoroethylene; polycarbonate; polyesters such aspolyethylene naphthalate, polyethylene terephthalate and the like;nylons such as nylon-6, nylon-6, 6, nylon-4, 10 and the like; polyimide;polysulfone; polyphenylene sulfide; silicon resins such as polydiphenylsiloxane and the like; phenol resins such as novolac and the like; epoxyresin; polyurethane; polystyrene, butadiene-styrene copolymer;polysaccharides such as cellulose, acetyl cellulose, nitrocellulose,starch, calcium alginate, hydroxypropyl methyl cellulose and the like;chitin; chitosan; urushi (Japanese lacquer); polyamides such as gelatin,collagen, keratin and the like; and copolymers of these high molecularmaterials

[0445] Further, the biochemical analysis unit 1 is constituted in theembodiment shown in FIGS. 19 and 20 by bringing the perforated plates142, 142 formed with a number of the through-holes 141 into closecontact with the both sides of the absorptive substrate 140 formed of anabsorptive material such as nylon-6. However, it is not absolutelynecessary to constitute the biochemical analysis unit 1 by abutting theperforated plates 142, 142 against both sides of the absorptivesubstrate 140 and the biochemical analysis unit 1 may be constituted byabutting the perforated plate 142 formed with a number of thethrough-holes 141 against only one surface of the absorptive substrate140.

[0446] Furthermore, in the above-described embodiments, biochemicalanalysis data are produced by reading radiation data of a radioactivelabeling substance recorded in a number of the dot-like stimulablephosphor layer regions 12 formed in the stimulable phosphor sheet 10 andfluorescence data of a fluorescent substance such as a fluorescent dyerecorded in the absorptive regions 4 formed in the through-holes 3 ofthe biochemical analysis unit 1 using the scanner shown in FIGS. 6 to13. However, it is not absolutely necessary to produce biochemicalanalysis data by reading radiation data of a radioactive labelingsubstance and fluorescence data of a fluorescent substance using asingle scanner and biochemical analysis data may be produced by readingradiation data of a radioactive labeling substance and fluorescence dataof a fluorescent substance using separate scanners.

[0447] Moreover, in the above-described embodiments, biochemicalanalysis data are produced by reading radiation data of a radioactivelabeling substance recorded in a number of the dot-like stimulablephosphor layer regions 12 formed in the stimulable phosphor sheet 10 andfluorescence data of a fluorescent substance such as a fluorescent dyerecorded in the absorptive regions 4 formed in the through-holes 3 ofthe biochemical analysis unit 1 using the scanner shown in FIGS. 6 to13. However, it is not absolutely necessary to read radiation data of aradioactive labeling substance using the scanner shown in FIGS. 6 to 13and any scanner constituted so as to scan and stimulate a number thedot-like stimulable phosphor layer regions 12 with a laser beam 24 maybe used for reading radiation data of a radioactive labeling substance.

[0448] Further, although the scanner shown in FIGS. 6 to 13 includes thefirst laser stimulating ray source 1, the second laser stimulating raysource 2 and the third laser stimulating ray source 3, it is notabsolutely necessary for the scanner to include three laser stimulatingray sources.

[0449] Furthermore, in the above-described embodiments, biochemicalanalysis data are produced by reading chemiluminescence data of alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate recorded in the absorptive regions4 formed in the through-holes 3 of the biochemical analysis unit 1 usingthe data producing system which can also read fluorescence data.However, it is not absolutely necessary to produce biochemical analysisdata by reading chemiluminescence data using the data producing systemwhich can also read fluorescence data and in the case where onlychemiluminescence data of a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substraterecorded in the absorptive regions 4 formed in the through-holes 3 ofthe biochemical analysis unit 1 are read, the light emitting diodestimulating ray source 100, the filter 101, the filter 102 and thediffusion plate 102 can be omitted from the data producing system.

[0450] Moreover, in the above-described embodiments, all of the dot-likestimulable phosphor layer regions 12 formed in the stimulable phosphorsheet 10 or the entire surface of the biochemical analysis unit 1 isscanned with a laser beam 24 to excite stimulable phosphor or afluorescent substance such as a fluorescent dye by moving the opticalhead 35 using a scanning mechanism in the X direction and the Ydirection in FIG. 12. However, all of the dot-like stimulable phosphorlayer regions 12 formed in the stimulable phosphor sheet 10 or theentire surface of the biochemical analysis unit 1 can be scanned with alaser beam 24 to excite stimulable phosphor or a fluorescent substancesuch as a fluorescent dye by moving the stage 40 in the X direction andthe Y direction in FIG. 12, while holding the stage 40 stationary.Further, the optical head 35 may be moved in one of the X direction andthe Y direction in FIG. 12, while the stage 40 is moved in the otherdirection.

[0451] Furthermore, although the perforated mirror 34 formed with thehole 33 is used in the scanner shown in FIGS. 6 to 13, the mirror can beformed with a coating capable of transmitting the laser beam 24 insteadof the hole 33.

[0452] Moreover, the photomultiplier 50 is employed as a light detectorto photoelectrically detect fluorescent light or stimulated emission inthe scanner shown in FIGS. 6 to 13. However, it is sufficient for thelight detector used in the present invention to be able tophotoelectrically detect fluorescent light or stimulated emission and itis possible to employ a light detector such as a line CCD or atwo-dimensional CCD instead of the photomultiplier 50.

[0453] Further, in the above-described embodiments, specific bindingsubstances such as cDNAs are spotted using the spotting device includingan injector 6 and a CCD camera 7 so that when the tip end portion of theinjector 6 and the center of the through-hole 3 or the through-hole 141into which a specific binding substance is to be spotted are determinedto coincide with each other as a result of viewing them using the CCDcamera 7, the specific binding substance such as cDNA is spotted fromthe injector 6. However, specific binding substances such as cDNAs canbe spotted by detecting the positional relationship between thethrough-holes 3 or the through-holes 141 formed in the biochemicalanalysis unit 1 and the tip end portion of the injector 6 in advance andtwo-dimensionally moving the biochemical analysis unit 1 or the tip endportion of the injector 6 so that the tip end portion of the injector 6coincides with each of the through-holes 3 or the through-holes 141.

[0454] Furthermore, although the spotting head 5 of the spotting deviceincludes the injector 6 for injecting a solution of specific bindingsubstances toward the biochemical analysis unit 1, 151 and the CCDcamera 7 in the above described embodiments, the spotting head 5 mayinclude, instead of the injector 6, a spotting pin for spotting specificbinding substances onto the biochemical analysis unit 1, 151.

[0455] Moreover, although the spotting head 5 of the spotting deviceincludes the CCD camera 7, it is not absolutely necessary for thespotting head 5 to include the CCD camera 7 and other solid-stateimaging devices such as a CID (charge injection device), a PDA(photodiode array), a MOS type imaging device and the like may be used.

[0456] Further, in the embodiment shown in FIGS. 21 to 24, although fourcorner portions of the biochemical analysis unit 151 are detected andthe coordinate values thereof are calculated using the referenceposition of the spotting head 5 as the origin of the coordinate system,it is sufficient to determine the relative positional relationshipbetween the biochemical analysis unit 151 and the spotting head 5 of thespotting device and it is not absolutely necessary to detect four cornerportions of the biochemical analysis unit 151 and calculate thecoordinate values thereof. It is possible to detect diagonally oppositecorner portions of the biochemical analysis unit 151, calculate thecoordinate values thereof using the reference position of the spottinghead 5 as the origin of the coordinate system, calculate drive pulses tobe sent to the main scanning pulse motor 166 and the sub-scanning pulsemotor 162, and move the spotting head 5.

[0457] Furthermore, in the embodiment shown in FIGS. 21 to 24, settingof the biochemical analysis unit 151 at a substantially constantposition on the base plate 160 is ensured by placing the biochemicalanalysis unit 151 on the base plate 160 so that two positioning pins177, 178 formed on the base plate 160 of the spotting device areinserted into two positioning through-holes 157, 158 of the biochemicalanalysis unit 151. Alternatively, three or more positioning pins may beformed on the base plate 160 and corresponding through-holes be formedin the biochemical analysis unit 151. Further, exact positioning of thebiochemical analysis unit 151 on the base plate 160 of the spottingdevice may be ensured, not by providing the two positioning pins 157,158, but instead by forming, for instance, a pair of guides having sideportions perpendicular to each other on the surface of the base plate160 of the spotting device and abutting side surfaces adjacent to thecorner portion of the biochemical analysis unit 151 against each of theguide.

[0458] Moreover, in the embodiment shown in FIGS. 21 to 24, the spottinghead 5 is moved in the main scanning direction and the sub-scanningdirection by moving the base plate 164 along the pair of rails 163, 163in the sub-scanning direction indicated by the arrow Y in FIG. 22 by thesub-scanning pulse motor 162 fixed on the frame 161 and intermittentlydriving the endless belt 167 at a predetermined pitch by the mainscanning pulse motor 166 provided on the movable base plate 164, therebymoving the spotting head 5 fixed on the endless belt 167 in the mainscanning direction indicated by the arrow X in FIG. 22. However, themechanism for driving the spotting head 5 is not limited to thisarrangement but the spotting head 5 may be moved in the main scanningdirection and the sub-scanning direction using any of variousappropriate mechanisms.

[0459] Further, in the embodiment shown in FIGS. 21 to 24, although thebiochemical analysis unit 151 is held stationary and the spotting head 5is moved in the main scanning direction and the sub-scanning directionwith respect to the biochemical analysis unit 151 placed on the baseplate 160, it is possible to hold the spotting head 5 stationary andmove the base plate 160 on which the biochemical analysis unit 151 isplaced in the main scanning direction and the sub-scanning direction.Moreover, it is also possible to move the spotting head 5 in the mainscanning direction or the sub-scanning direction and move the base plate160 on which the biochemical analysis unit 151 is placed in thesub-scanning direction or the main scanning direction.

[0460] Furthermore, in the embodiment shown in FIGS. 21 to 24, since anumber of the absorptive regions 155 are regularly formed in thebiochemical analysis unit 151, the spotting head 5 is moved at constantpitches without using the CCD camera after the coordinate values of anumber of the absorptive regions 155 are determined using the CCD camera7 in the coordinate system in which the reference position of thespotting head 5 is used as the origin thereof However, for instance, inthe case where a number of the absorptive regions 155 are not regularlyformed in the biochemical analysis unit 151, it is possible to spotspecific binding substances by confirming the position to which specificbinding substances are to be spotted using the CCD camera 7 while thespotting head 5 is being moved.

[0461] Moreover, in the above described embodiments, the scanner isprovided with the first laser stimulating ray source 21 for emitting alaser beam having a wavelength of 640 nm, the second laser stimulatingray source 22 for emitting a laser beam having a wavelength of 532 nmand the third laser stimulating ray source 23 for emitting a laser beamhaving a wavelength of 473 nm. However, it is not absolutely necessaryto use a laser stimulating ray source as a stimulating ray source and alight emitting diode stimulating ray source may be used as a stimulatingray source instead of any of the laser stimulating ray sources. Further,a halogen ramp may be used as any of the stimulating ray source providedthat light components of a wavelength that does not contribute tostimulation are cut by a spectral filter.

[0462] According to the present invention, it is possible to provide abiochemical analysis unit which can prevent noise caused by thescattering of electron beams released from a radioactive labelingsubstance from being generated in biochemical analysis data even in thecase of forming spots of specific binding substances on the surface of acarrier at high density, which can specifically bind with a substancederived from a living organism and whose sequence, base length,composition and the like are known, specifically binding the spot-likespecific binding substances with a substance derived from a livingorganism labeled with a radioactive substance to selectively label thespot-like specific binding substances with a radioactive substance,thereby obtaining a biochemical analysis unit, superposing the thusobtained biochemical analysis unit and a stimulable phosphor layertogether, exposing the stimulable phosphor layer to a radioactivelabeling substance, irradiating the stimulable phosphor layer with astimulating ray to excite the stimulable phosphor, photoelectricallydetecting the stimulated emission released from the stimulable phosphorlayer to produce biochemical analysis data, and analyzing the substancederived from a living organism.

[0463] Further, according to the present invention, it is possible toprovide a biochemical analysis unit which can prevent noise caused bythe scattering of chemiluminescent emission and/or fluorescence releasedfrom a labeling substance which generates chemiluminescent emission whenit contacts a chemiluminescent substrate and/or a fluorescent substancefrom being generated in biochemical analysis data even in the case offorming spots of specific binding substances on the surface of a carrierat high density, which can specifically bind with a substance derivedfrom a living organism and whose sequence, base length, composition andthe like are known, specifically binding the spot-like specific bindingsubstances with a substance derived from a living organism labeled with,in addition to a radioactive labeling substance or instead of aradioactive labeling substance, a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand/or a fluorescent substance to selectively label the spot-likespecific binding substances therewith, thereby obtaining a biochemicalanalysis unit, photoelectrically detecting chemiluminescent emissionand/or fluorescence released from the biochemical analysis unit toproduce biochemical analysis data, and analyzing the substance derivedfrom a living organism.

[0464] Furthermore, according to the present invention, it is possibleto provide a biochemical analyzing method which can effect quantitativebiochemical analysis with high accuracy by producing biochemicalanalysis data based on a biochemical analysis unit obtained by formingspots of a specific binding substance on the surface of a carrier athigh density, which can specifically bind with a substance derived froma living organism and whose sequence, base length, composition and thelike are known, specifically binding the spot-like specific bindingsubstances with a substance derived from a living organism labeled witha radioactive labeling substance, a labeling substance which generateschemiluminescent emission when it contacts a chemiluminescent substrateand/or a fluorescent substance, thereby selectively labeling thespot-like specific binding substances therewith.

1. A biochemical analysis unit comprising a substrate made of a materialcapable of attenuating radiation energy and/or light energy and formedwith a plurality of holes, and a plurality of absorptive regions formedby forming an absorptive region in every hole.
 2. A biochemical analysisunit comprising a substrate made of a material capable of attenuatingradiation energy and/or light energy and formed with a plurality ofholes, and a plurality of absorptive regions formed by forming anabsorptive region in every hole, the plurality of absorptive regionsbeing selectively labeled with at least one kind of labeling substanceselected from a group consisting of a radioactive labeling substance, alabeling substance which generates chemiluminescent emission when itcontacts a chemiluminescent substrate and a fluorescent substance byspotting specific binding substances whose sequence, base length,composition and the like are known therein and specifically binding asubstance derived from a living organism and labeled with at least onekind of said labeling substance with the specific binding substances. 3.A biochemical analysis unit in accordance with claim 2 wherein thesubstance derived from a living organism is specifically bound withspecific binding substances by a reaction selected from a groupconsisting of hybridization, antigen-antibody reaction andreceptor-ligand reaction.
 4. A biochemical analysis unit in accordancewith claim 1 wherein the plurality of absorptive regions are formed bycharging an absorptive material in the plurality of holes formed in thesubstrate.
 5. A biochemical analysis unit in accordance with claim 2wherein the plurality of absorptive regions are formed by charging anabsorptive material in the plurality of holes formed in the substrate.6. A biochemical analysis unit in accordance with claim 1 wherein eachof the plurality of holes is formed as a through-hole.
 7. A biochemicalanalysis unit in accordance with claim 2 wherein each of the pluralityof holes is formed as a through-hole.
 8. A biochemical analysis unit inaccordance with claim 1 wherein each of the plurality of holes is formedas a recess.
 9. A biochemical analysis unit in accordance with claim 2wherein each of the plurality of holes is formed as a recess.
 10. Abiochemical analysis unit in accordance with claim 1 wherein thesubstrate is formed of a flexible material.
 11. A biochemical analysisunit in accordance with claim 2 wherein the substrate is formed of aflexible material.
 12. A biochemical analysis unit in accordance withclaim 1 wherein the substrate is formed with a gripping portion by whichthe substrate can be gripped.
 13. A biochemical analysis unit inaccordance with claim 2 wherein the substrate is formed with a grippingportion by which the substrate can be gripped.
 14. A biochemicalanalysis unit comprising an absorptive substrate formed of an absorptivematerial and a perforated plate formed with a plurality of through-holesand made of a material capable of attenuating radiation energy and lightenergy, the perforated plate being closely contacted with at least onesurface of the absorptive substrate to form a plurality of absorptiveregions of the absorptive substrate in the plurality of through-holesformed in the perforated plate.
 15. A biochemical analysis unit inaccordance with claim 14 wherein perforated plates are in close contactwith the both surfaces of the absorptive substrate.
 16. A biochemicalanalysis unit in accordance with claim 14 wherein the perforated plateis formed with a gripping portion by which the perforated plate can begripped.
 17. A biochemical analysis unit in accordance with claim 14wherein the plurality of absorptive regions are selectively labeled withat least one kind of labeling substances selected from a groupconsisting of a radioactive labeling substance, a labeling substancecapable of generating chemiluminescent emission when it contacts achemiluminescent substrate and/or a fluorescent substance by spottingspecific binding substances whose sequence, base length, composition andthe like are known therein and hybridizing a substance derived from aliving organism and labeled with at least one kind of labeling substancewith the specific binding substances.
 18. A biochemical analysis unit inaccordance with claim 1 which is formed with 10 or more holes.
 19. Abiochemical analysis unit in accordance with claim 2 which is formedwith 10 or more holes.
 20. A biochemical analysis unit in accordancewith claim 14 which is formed with 10 or more holes.
 21. A biochemicalanalysis unit in accordance with claim 18 which is formed with 1,000 ormore holes.
 22. A biochemical analysis unit in accordance with claim 19which is formed with 1,000 or more holes.
 23. A biochemical analysisunit in accordance with claim 20 which is formed with 1,000 or moreholes.
 24. A biochemical analysis unit in accordance with claim 21 which10,000 or more holes.
 25. A biochemical analysis unit in accordance withclaim 22 which 10,000 or more holes.
 26. A biochemical analysis unit inaccordance with claim 23 which 10,000 or more holes.
 27. A biochemicalanalysis unit in accordance with claim 1 wherein each of the pluralityof holes has a size of less than 5 mm².
 28. A biochemical analysis unitin accordance with claim 2 wherein each of the plurality of holes has asize of less than 5 mm².
 29. A biochemical analysis unit in accordancewith claim 14 wherein each of the plurality of holes has a size of lessthan 5 mm².
 30. A biochemical analysis unit in accordance with claim 27wherein each of the plurality of holes has a size of less than 1 mm².31. A biochemical analysis unit in accordance with claim 28 wherein eachof the plurality of holes has a size of less than 1 mm².
 32. Abiochemical analysis unit in accordance with claim 29 wherein each ofthe plurality of holes has a size of less than 1 mm².
 33. A biochemicalanalysis unit in accordance with claim 30 wherein each of the pluralityof holes has a size of less than 0.1 mm².
 34. A biochemical analysisunit in accordance with claim 31 wherein each of the plurality of holeshas a size of less than 0.1 mm².
 35. A biochemical analysis unit inaccordance with claim 32 wherein each of the plurality of holes has asize of less than 0.1 mm².
 36. A biochemical analysis unit in accordancewith claim 1 wherein the plurality of holes are formed at a density of10 or more per cm².
 37. A biochemical analysis unit in accordance withclaim 2 wherein the plurality of holes are formed at a density of 10 ormore per cm².
 38. A biochemical analysis unit in accordance with claim14 wherein the plurality of holes are formed at a density of 10 or moreper cm².
 39. A biochemical analysis unit in accordance with claim 36wherein the plurality of holes are formed at a density of 1,000 or moreper cm².
 40. A biochemical analysis unit in accordance with claim 37wherein the plurality of holes are formed at a density of 1,000 or moreper cm².
 41. A biochemical analysis unit in accordance with claim 38wherein the plurality of holes are formed at a density of 1,000 or moreper cm².
 42. A biochemical analysis unit in accordance with claim 39wherein the plurality of holes are formed at a density of 10,000 or moreper cm².
 43. A biochemical analysis unit in accordance with claim 40wherein the plurality of holes are formed at a density of 10,000 or moreper cm².
 44. A biochemical analysis unit in accordance with claim 41wherein the plurality of holes are formed at a density of 10,000 or moreper cm².
 45. A biochemical analysis unit in accordance with claim 1wherein the material capable of attenuating radiation energy and/orlight energy has a property of reducing the energy of radiation and/orlight to ⅕ or less when the radiation and/or light travels in thematerial by a distance equal to that between neighboring absorptiveregions.
 46. A biochemical analysis unit in accordance with claim 2wherein the material capable of attenuating radiation energy and/orlight energy has a property of reducing the energy of radiation and/orlight to ⅕ or less when the radiation and/or light travels in thematerial by a distance equal to that between neighboring absorptiveregions.
 47. A biochemical analysis unit in accordance with claim 14wherein the material capable of attenuating radiation energy and/orlight energy has a property of reducing the energy of radiation and/orlight to ⅕ or less when the radiation and/or light travels in thematerial by a distance equal to that between neighboring absorptiveregions.
 48. A biochemical analysis unit in accordance with 45 whereinthe material capable of attenuating radiation energy and/or light energyhas a property of reducing the energy of radiation and/or light to{fraction (1/10)} or less when the radiation and/or light travels in thematerial by a distance equal to that between neighboring absorptiveregions.
 49. A biochemical analysis unit in accordance with 46 whereinthe material capable of attenuating radiation energy and/or light energyhas a property of reducing the energy of radiation and/or light to{fraction (1/10)} or less when the radiation and/or light travels in thematerial by a distance equal to that between neighboring absorptiveregions.
 50. A biochemical analysis unit in accordance with 47 whereinthe material capable of attenuating radiation energy and/or light energyhas a property of reducing the energy of radiation and/or light to{fraction (1/10)} or less when the radiation and/or light travels in thematerial by a distance equal to that between neighboring absorptiveregions.
 51. A biochemical analysis unit in accordance with 48 whereinthe material capable of attenuating radiation energy and/or light energyhas a property of reducing the energy of radiation and/or light to{fraction (1/100)} or less when the radiation and/or light travels inthe material by a distance equal to that between neighboring absorptivephosphor layer regions.
 52. A biochemical analysis unit in accordancewith 49 wherein the material capable of attenuating radiation energyand/or light energy has a property of reducing the energy of radiationand/or light to {fraction (1/100)} or less when the radiation and/orlight travels in the material by a distance equal to that betweenneighboring absorptive phosphor layer regions.
 53. A biochemicalanalysis unit in accordance with 50 wherein the material capable ofattenuating radiation energy and/or light energy has a property ofreducing the energy of radiation and/or light to {fraction (1/100)} orless when the radiation and/or light travels in the material by adistance equal to that between neighboring absorptive phosphor layerregions.
 54. A biochemical analysis unit in accordance with claim 45wherein the substrate is formed of a material selected from a groupconsisting of metal material, ceramic material and plastic material. 55.A biochemical analysis unit in accordance with claim 46 wherein thesubstrate is formed of a material selected from a group consisting ofmetal material, ceramic material and plastic material.
 56. A biochemicalanalysis unit in accordance with claim 47 wherein the substrate isformed of a material selected from a group consisting of metal material,ceramic material and plastic material.
 57. A biochemical analysis unitin accordance with claim 1 wherein the absorptive region is formed of aporous material.
 58. A biochemical analysis unit in accordance withclaim 2 wherein the absorptive region is formed of a porous material.59. A biochemical analysis unit in accordance with claim 14 wherein theabsorptive substrate is formed of a porous material.
 60. A biochemicalanalysis unit in accordance with claim 57 wherein the porous materialincludes a carbon material or a material capable of forming a membranefilter.
 61. A biochemical analysis unit in accordance with claim 58wherein the porous material includes a carbon material or a materialcapable of forming a membrane filter.
 62. A biochemical analysis unit inaccordance with claim 59 wherein the porous material includes a carbonmaterial or a material capable of forming a membrane filter.
 63. Abiochemical analysis unit in accordance with claim 1 wherein theabsorptive region is formed of a fiber material.
 64. A biochemicalanalysis unit in accordance with claim 2 wherein the absorptive regionis formed of a fiber material.
 65. A biochemical analysis unit inaccordance with claim 14 wherein the absorptive substrate is formed of afiber material.
 66. A biochemical analyzing method comprising the stepsof preparing a biochemical analysis unit by spotting specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, in a plurality of absorptive regions, each of which isformed in a plurality of holes formed in a substrate made of a materialcapable of attenuating radiation energy and specifically binding asubstance derived from a living organism and labeled with a radioactivelabeling substance with the specific binding substances, superposing thebiochemical analysis unit on a stimulable phosphor sheet in which astimulable phosphor layer is formed so that the stimulable phosphorlayer faces the plurality of absorptive regions, thereby exposing thestimulable phosphor layer to the radioactive labeling substancecontained in the plurality of absorptive regions, irradiating thestimulable phosphor layer exposed to the radioactive labeling substancewith a stimulating ray, thereby exciting stimulable phosphor containedin the stimulable phosphor layer, photoelectrically detecting stimulatedemission released from the stimulable phosphor contained in thestimulable phosphor layer, thereby producing biochemical analysis data,and effecting biochemical analysis based on the biochemical analysisdata.
 67. A biochemical analyzing method in accordance with claim 66wherein a plurality of dot-like stimulable phosphor layer regions areformed spaced-apart from each other in the stimulable phosphor sheet inthe same pattern as that of the plurality of holes formed in thesubstrate of the biochemical analysis unit and the biochemical analysisunit and the stimulable phosphor sheet are superposed on each other sothat each of the plurality of dot-like stimulable phosphor layer regionsfaces one of the plurality of absorptive regions in the plurality ofholes formed in the substrate of the biochemical analysis unit, therebyexposing the plurality of dot-like stimulable phosphor layer regions ofthe stimulable phosphor sheet to the radioactive labeling substancecontained in the plurality of absorptive regions.
 68. A biochemicalanalyzing method comprising the steps of preparing a biochemicalanalysis unit comprising an absorptive substrate formed of an absorptivematerial and a perforated plate made of a material capable ofattenuating radiation energy and light energy and formed with aplurality of through-holes, the perforated plate being closely contactedwith at least one surface of the absorptive substrate to form aplurality of absorptive regions of the absorptive substrate in theplurality of through-holes formed in the perforated plate, the pluralityof absorptive regions being selectively labeled with a radioactivelabeling substance by spotting specific binding substances, which canspecifically bind with a substance derived from a living organism andwhose sequence, base length, composition and the like are known, in theplurality of absorptive regions and specifically binding a substancederived from a living organism and labeled with a radioactive labelingsubstance, superposing the biochemical analysis unit and a stimulablephosphor sheet in which a stimulable phosphor layer is formed via theperforated plate so that the stimulable phosphor layer faces theplurality of absorptive regions, thereby exposing the stimulablephosphor layer to the radioactive labeling substance contained in theplurality of absorptive regions, irradiating the stimulable phosphorlayer exposed to the radioactive labeling substance with a stimulatingray to excite stimulable phosphor contained in the stimulable phosphorlayer, photoelectrically detecting stimulated emission released from thestimulable phosphor contained in the stimulable phosphor layer toproduce biochemical analysis data, and effecting biochemical analysisbased on the biochemical analysis data.
 69. A biochemical analyzingmethod in accordance with claim 68 wherein a plurality of dot-likestimulable phosphor layer regions are formed spaced-apart in thestimulable phosphor sheet in the same pattern as that of the pluralityof through-holes formed in the perforated plate, and the biochemicalanalysis unit and the stimulable phosphor sheet are superposed on eachother so that each of the plurality of dot-like stimulable phosphorlayer regions faces one of the plurality of absorptive regions via oneof the through-holes formed in the perforated plate, thereby exposingthe plurality of dot-like stimulable phosphor layer regions to aradioactive labeling substance contained in the plurality of absorptiveregions.
 70. A biochemical analyzing method comprising the steps ofpreparing a biochemical analysis unit by spotting specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, in a plurality of absorptive regions formed in aplurality of holes formed in a substrate made of a material capable ofattenuating light energy and specifically binding a substance derivedfrom a living organism and labeled with a fluorescent substance with thespecific binding substances, thereby selectively labeling a plurality ofabsorptive regions, irradiating the biochemical analysis unit with astimulating ray, thereby exciting the fluorescent substance,photoelectrically detecting fluorescence released from the fluorescentsubstance, thereby producing biochemical analysis data, and effectingbiochemical analysis based on the biochemical analysis data.
 71. Abiochemical analyzing method comprising the steps of preparing abiochemical analysis unit by spotting specific binding substances, whichcan specifically bind with a substance derived from a living organismand whose sequence, base length, composition and the like are known, ina plurality of absorptive regions formed in a plurality of holes formedin a substrate made of a material capable of attenuating light energyand specifically binding a substance derived from a living organism andlabeled with a labeling substance capable of generating chemiluminescentemission when it contacts a chemiluminescent substrate with the specificbinding substances, thereby selectively labeling the plurality ofabsorptive regions, bringing the biochemical analysis unit into closecontact with a chemiluminescent substrate, photoelectrically detectingchemiluminescent emission released from the labeling substance, therebyproducing biochemical analysis data, and effecting biochemical analysisbased on the biochemical analysis data.
 72. A biochemical analyzingmethod comprising the steps of preparing a biochemical analysis unit byspotting specific binding substances, which can specifically bind with asubstance derived from a living organism and whose sequence, baselength, composition and the like are known, in a plurality of absorptiveregions formed in a plurality of holes formed in a substrate made of amaterial capable of attenuating light energy and specifically binding asubstance derived from a living organism and labeled with a fluorescentsubstance and a labeling substance capable of generatingchemiluminescent emission when it contacts a chemiluminescent substratewith the specific binding substances, thereby selectively labeling theplurality of absorptive regions, irradiating the biochemical analysisunit with a stimulating ray to excite the fluorescent substance, andphotoelectrically detecting fluorescence released from the fluorescentsubstance, thereby producing biochemical analysis data, while bringingthe biochemical analysis unit into close contact with a chemiluminescentsubstrate, photoelectrically detecting chemiluminescent emissionreleased from the labeling substance, thereby producing biochemicalanalysis data, and effecting biochemical analysis based on thebiochemical analysis data.
 73. A biochemical analyzing method comprisingthe steps of bringing an absorptive substrate made of an absorptivematerial and formed with a plurality of absorptive regions by spottingthereon specific binding substances, which can specifically bind with asubstance derived from a living organism and whose sequence, baselength, composition and the like are known, the plurality of theabsorptive regions being selectively labeled by specifically binding asubstance derived from a living organism and labeled with a fluorescentsubstance with the specific binding substances contained in theplurality of absorptive regions, into close contact with a perforatedplate made of a material capable of attenuating light energy and formedwith a plurality of through-holes at positions corresponding to theplurality of absorptive regions formed in the absorptive substrate,irradiating the plurality of absorptive regions formed in the absorptivesubstrate through the plurality of through-holes formed in theperforated plate to stimulate the fluorescent substance,photoelectrically detecting fluorescence released from the fluorescentsubstance, thereby producing biochemical analysis data, and effectingbiochemical analysis based on the biochemical analysis data.
 74. Abiochemical analyzing method comprising the steps of bringing anabsorptive substrate made of an absorptive material and formed with aplurality of absorptive regions by spotting thereon specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, the plurality of the absorptive regions beingselectively labeled by specifically binding a substance derived from aliving organism and labeled with a labeling substance capable ofgenerating chemiluminescent emission when it contacts a chemiluminescentsubstrate with the specific binding substances contained in theplurality of absorptive regions, into close contact with a perforatedplate made of a material capable of attenuating light energy and formedwith a plurality of through-holes at positions corresponding to theplurality of absorptive regions formed in the absorptive substrate,bringing a chemiluminescent substrate into close contact with theplurality of absorptive regions formed in the absorptive substratethrough the plurality of through-holes formed in the perforated plate,photoelectrically detecting chemiluminescent emission released from thelabeling substance, thereby producing biochemical analysis data, andeffecting biochemical analysis based on the biochemical analysis data.75. A biochemical analyzing method comprising the steps of bringing anabsorptive substrate made of an absorptive material and formed with aplurality of absorptive regions by spotting thereon specific bindingsubstances, which can specifically bind with a substance derived from aliving organism and whose sequence, base length, composition and thelike are known, the plurality of the absorptive regions beingselectively labeled by specifically binding a substance derived from aliving organism and labeled with a fluorescent substance and a labelingsubstance capable of generating chemiluminescent emission when itcontacts a chemiluminescent substrate with the specific bindingsubstances contained in the plurality of absorptive regions, into closecontact with a perforated plate made of a material capable ofattenuating light energy and formed with a plurality of through-holes atpositions corresponding to the plurality of absorptive regions formed inthe absorptive substrate, irradiating the plurality of absorptiveregions formed in the absorptive substrate through the plurality ofthrough-holes formed in the perforated plate to stimulate thefluorescent substance, and photoelectrically detecting fluorescencereleased from the fluorescent substance, thereby producing biochemicalanalysis data, while bringing a chemiluminescent substrate into closecontact with the plurality of absorptive regions formed in theabsorptive substrate through the plurality of through-holes formed inthe perforated plate, and photoelectrically detecting chemiluminescentemission released from the labeling substance, thereby producingbiochemical analysis data, and effecting biochemical analysis based onthe biochemical analysis data.
 76. A biochemical analyzing methodcomprising the steps of preparing a stimulable phosphor sheet includinga support, selectively storing radiation energy in a plurality ofstimulable phosphor layer regions formed at least one-dimensionally andspaced-apart from each other in the support, moving the stimulablephosphor sheet and a stimulating ray relative to each other in at leasta main scanning direction, sequentially irradiating the plurality ofstimulable phosphor layer regions with the stimulating ray, therebyexciting stimulable phosphor contained in the plurality of stimulablephosphor layer regions, photoelectrically detecting stimulated emissionreleased from the stimulable phosphor, thereby producing analog data,converting the analog data to digital data and producing biochemicalanalysis data.